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Team:NEAU-Harbin/protocols.html
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<h3 style="padding-left:20px">1.PCR amplification</h3> | <h3 style="padding-left:20px">1.PCR amplification</h3> | ||
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<p style="padding-left:20px">① PCR amplification of amilCP (BBa_K592009)<br> | <p style="padding-left:20px">① PCR amplification of amilCP (BBa_K592009)<br> | ||
Primers: <br> | Primers: <br> | ||
Revision as of 02:19, 16 October 2014
PROTOCOLS
1.PCR amplification
① PCR amplification of amilCP (BBa_K592009)
Primers:
Sense: GGGCCCATGAGTGTGATCGCTAAACAAAT
Antisense: GGTACCAAGCTTAGGCGACCACAGGTTTG
Reaction system:
PCR condition:
② PCR amplification of cjBlue (BBa_K592011)
Primers:
Sense: GAATTCGGTAGCGGCTCCGGAAGCGGTTCCGGCAGCatggcttccaaaataagcg
Antisense: GGTACCAAGCTTAGGCGACCACAGGTTTG
Reaction system:
PCR condition
③ PCR amplification of GlaA3
Primers:
Sense: AGATCT ACAATCAATCCATTTCGCTATAG
Antisense: TCTAGA CATAAGGCGGGTTCACATC
Reaction system:
