Team:Hannover/Protocols/Detection/Confocal

From 2014.igem.org

(Difference between revisions)
(Created page with "{{Template:Team:Hannover/igem.css}} {{Template:Team:Hannover/main.css}} {{Template:Team:Hannover/main.tmpl}} {{Template:Team:Hannover/newmenu.tmpl}} <html> <body> <div id='main_c...")
Line 8: Line 8:
<h1><a href="https://2014.igem.org/Team:Hannover/Protocols" target="_blank">Protocols</a> / How To: Confocal Microscopy</h1>
<h1><a href="https://2014.igem.org/Team:Hannover/Protocols" target="_blank">Protocols</a> / How To: Confocal Microscopy</h1>
-
<p><h4>Protocol:</h4></p>
+
<table colspan="2"><tr><td><h4>Material:</h4></td>
-
<p class="text">After induction of heterologous T4MBP expression, bacterial cell cultures were centrifuged for 15 min at 4500 x g and 4 °C. While the supernatant was decanted, the precipitated bacteria were dissolved in 20 ml isosmotic buffer. According to the harm of heavy metals, all medium supernatants were stored and had to be picked up by professional disposal service. Starting with the dissolving the precipitate, the washing is repeated four more times. Due to the heavy metals, the supernatants obtained here had to be stored also. As the last step, precipitated bacteria were dissolved in 1 ml H20 and dried for 2 d at 60 °C. For latter MS, the reaction tube´s dry-weight before and after loading the aqueous bacteria solution had to be determined.</p>
+
<tr><td>Prepared MS Samples</td><td></td></tr>
 +
<tr><td>Confocal Microscope</td><td>(here:Eclipse TE2000-E, Nikon, Japan)</td></tr></table>
 +
<h2>Settings</h2><p class="text"><ol><li></ol></p>
 +
<br><br>
 +
<h2>Protocol</h2>
 +
<p class="text">The exponential megaprimer PCR (EMP)</p>
-
</div>
 
-
<div id='side_content'>
 
-
<div class='annotation' ref='a1'>
 
-
Humor is always individual. For a presentation entertaining as multiple humors as possible meet with several team members and do a brainstorming session.</div>
 
-
</div>
 
<p id="Fusszeile"><br><br><br><br><img src="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141010Fusszeile2.jpg" width="980px" style="float:right" /></p>
<p id="Fusszeile"><br><br><br><br><img src="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141010Fusszeile2.jpg" width="980px" style="float:right" /></p>
</body>
</body>
</html>
</html>

Revision as of 15:21, 15 October 2014

Protocols / How To: Confocal Microscopy

Material:
Prepared MS Samples
Confocal Microscope(here:Eclipse TE2000-E, Nikon, Japan)

Settings



Protocol

The exponential megaprimer PCR (EMP)





Retrieved from "http://2014.igem.org/Team:Hannover/Protocols/Detection/Confocal"