Team:NU Kazakhstan/Modeling

From 2014.igem.org

(Difference between revisions)
Line 26: Line 26:
     border: 0px solid #8B4513;  
     border: 0px solid #8B4513;  
     display: inline-block;
     display: inline-block;
-
     padding: 13px 21px;  
+
     padding: 10px 14px;  
     text-decoration: none;  
     text-decoration: none;  
     color: #000;  
     color: #000;  
Line 48: Line 48:
</div>
</div>
-
<div style="position:absolute; top:425px; width:940px">
+
<div style="position:absolute; top:460px; width:940px">
<table border="0" align="center" cellpadding="0" cellspacing="0">
<table border="0" align="center" cellpadding="0" cellspacing="0">
   <tr>
   <tr>
Line 67: Line 67:
    
    
     <td class="c1"><a href="https://2014.igem.org/Team:NU_Kazakhstan/Safety">Safety</a></td>
     <td class="c1"><a href="https://2014.igem.org/Team:NU_Kazakhstan/Safety">Safety</a></td>
 +
 
 +
    <td class="c1"><a href="https://2014.igem.org/Team:NU_Kazakhstan/Human practices">Human practices</a></td>
      
      
     <td class="c1"><a href="https://2014.igem.org/Team:NU_Kazakhstan/Interlab Study">Interlab Study</a></td>
     <td class="c1"><a href="https://2014.igem.org/Team:NU_Kazakhstan/Interlab Study">Interlab Study</a></td>
       <td class="c1"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"  
       <td class="c1"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"  
-
width="50px"></a> </td>
+
width="40px"></a> </td>
</tr>
</tr>
   </table>
   </table>
Line 82: Line 84:
         <td valign="top" class="body_txt"><h1></h1>
         <td valign="top" class="body_txt"><h1></h1>
<script type="text/javascript"></script>
<script type="text/javascript"></script>
 +
           <br>
           <br>
<br>
<br>

Revision as of 02:09, 15 October 2014






Nanobodies

Plasmid design

  • RFP – engineered mutant of red fluorescent protein from Discosoma striata (coral)
  • HlyA- C-terminal signal sequence of alpha-hemolysin

    • The construct was synthesized by GenScript company in pUC57 vector

    We inserted the construct into the pSB1C3 plasmid into the standard restriction sites of EcoRI and PstI

    Introducing permanent competence into E. coli

    Making construct

    The gene for Gp16 ATP-ase protein was ordered from GenScript company.Then, it was combined with the constitutive Anderson promoter + INP, and the constructed part was cloned into standard pSB1C3 plasmid with Circular polymerase extension cloning (CPEC).

    References

    Fraile, S., Muñoz, A., De Lorenzo, V., & Fernández, L. A. (2004). Secretion of proteins with dimerization capacity by the haemolysin type I transport system of Escherichia coli. Molecular microbiology, 53(4), 1109-1121

    Schwartz C, De Donatis GM, Fang H, Guo P. (2013). The ATPase of the phi29 DNA packaging motor is a member of the hexameric AAA+ superfamily. Virology. 443: 20–27.

    Quan J, Tian J. (2009) Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways. PLoS ONE 4(7): e6441. doi:10.1371/journal.pone.0006441

    Retrieved from "http://2014.igem.org/Team:NU_Kazakhstan/Modeling"