Team:Austin Texas/kit
From 2014.igem.org
(→Using The Kit) |
(→Using The Kit) |
||
Line 140: | Line 140: | ||
However, the above scenarios assume that the synthetase/tRNA pair function efficiently and with high fidelity. | However, the above scenarios assume that the synthetase/tRNA pair function efficiently and with high fidelity. | ||
*Actual synthetase/tRNA pairs sometimes have '''low fidelity''', leading to the incorporation of an incorrect amino acid. Such a pair would not give results that mimic Figure 2C, expression of only RFP. Rather, in the absence of ncAA, such a pair would give results similar to Figure 2D, full expression of RFP and sfGFP. | *Actual synthetase/tRNA pairs sometimes have '''low fidelity''', leading to the incorporation of an incorrect amino acid. Such a pair would not give results that mimic Figure 2C, expression of only RFP. Rather, in the absence of ncAA, such a pair would give results similar to Figure 2D, full expression of RFP and sfGFP. | ||
+ | |||
*Actual synthetase/tRNA pairs can also sometimes have '''low efficiency'''. As these are artificially selected pairs, sometimes they do not function as well as natural synthetase/tRNA pairs. In such a case, the amount of sfGFP translated in Figure 2D might be significantly lower than expected. | *Actual synthetase/tRNA pairs can also sometimes have '''low efficiency'''. As these are artificially selected pairs, sometimes they do not function as well as natural synthetase/tRNA pairs. In such a case, the amount of sfGFP translated in Figure 2D might be significantly lower than expected. | ||
Revision as of 19:55, 14 October 2014
|