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Team:UGA-Georgia/Protocols
From 2014.igem.org
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<tr><td colspan="3"> <h3>PCR</h3></td></tr> | <tr><td colspan="3"> <h3>PCR</h3></td></tr> | ||
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| + | <li>Amplified mCherry gene with negative control group and positive control group</li> | ||
| + | <li>Reaction Mixture (a master mix to be divided into 3 tubes; a. Mq water 10.2 uL X 4 = 40.8 uL, b. 2x Phusion polymerase HF buffer mix 12.5 uL x 4 = 50 uL, c. Gene 0.5 uL x 4 = 2 uL, d. Forward primer 0.9 uL X 4= 3.6 uL, e. Reverse primer 0.9 uL x 4 = 3.6 uL </li> | ||
| + | <li>Final Reaction Mixture = 96.4 uL with 24.1 uL in each tube</li> | ||
| + | <li>Annealing temperature = 65 Celsius </li> | ||
| + | <li>Run products on a gel for verification (2.5 uL of each)</li> | ||
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| + | </ol> | ||
</p> | </p> | ||
</td> | </td> | ||
Revision as of 00:21, 13 October 2014
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