Revision as of 17:12, 2 October 2014

TEC-CEM | Project

ITESM-CEM | Medical Bioremediation

Project 3014

_{Project Description}

_{Project Details}

_{Materials & Methods}

_{The Experiments}

_Results

_{Data analysis}

_{Human Practices}

_Conclusions

The Experiments

If you choose to create a model during your project, please write about it here. Modeling is not an essential part of iGEM, but we encourage any and all teams to model some aspect of their project. See previous "Best Model" awards for more information.

Experiment One

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vivamus fringilla porta nisi sed dictum. Aliquam at rutrum nisl. Ut eros quam, condimentum sed pretium et, euismod vitae tellus. Curabitur eu blandit massa. Vestibulum at euismod purus. Sed quis pretium ligula. Cras non tristique nulla. Nunc finibus risus non purus malesuada viverra. Cras bibendum augue eu lorem faucibus, nec auctor mi iaculis. Vivamus sagittis, mi non gravida accumsan, odio dui pellentesque leo, a varius dui mauris eu diam. Maecenas laoreet tellus sed porta efficitur.

Experiment Two

Etiam tempus mi pulvinar purus iaculis bibendum. Vivamus vel risus eu enim volutpat finibus. Nullam bibendum est sit amet arcu lobortis, id laoreet ex vestibulum. Curabitur fringilla eleifend lacus, nec ornare nibh imperdiet sed. Maecenas vel velit consectetur, tempus libero quis, consectetur ex. Nulla porttitor pharetra velit. Curabitur tristique, dolor ut sodales euismod, diam diam ultricies arcu, at tincidunt tellus neque in felis. Etiam ut tempor ligula. Sed at dui sapien.

Experiment Three

Proin aliquam nibh id elementum pellentesque. Suspendisse mollis est ut felis sagittis mollis. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Etiam accumsan ex ante, quis lobortis erat fermentum ac. Sed et egestas libero. Donec id diam vitae leo consequat interdum. Ut in sem in quam pretium finibus vitae non lectus.

Experiment Four

Curabitur sed erat ante. Maecenas viverra odio sit amet ultrices facilisis. Nullam lobortis mi quam, sit amet placerat erat eleifend eget. Mauris commodo sem tortor, quis blandit orci laoreet vel. In hac habitasse platea dictumst. Curabitur molestie eu dolor ac bibendum. Duis fermentum tellus et dui dapibus, sed feugiat dolor sollicitudin. Nulla malesuada, tortor quis blandit dictum, odio risus rutrum sapien, varius pharetra dui tellus sed lectus. Sed eget magna at justo tempus hendrerit. Nulla in odio nec libero maximus finibus.

@@ Line 22: / Line 22: @@
 <table border="0" align="center" cellpadding="0" cellspacing="0" id="Table_01" style="background:transparent;">
    <tr>
-     <td colspan="3" valign="bottom"><h3>ITESM-CEM | Enzy7-K me</h3></td>
+     <td colspan="3" valign="bottom"><h3>ITESM-CEM | Medical Bioremediation</h3></td>
      <td colspan="2" rowspan="2" align="right">
@@ Line 48: / Line 48: @@
    </tr>
    <tr>
-     <td colspan="3" rowspan="3" align="left" valign="top"><ul>
+     <td colspan="3" rowspan="2" align="left" valign="top"><ul>
-       <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Project" style="color: #FFF;">Project Description</a></sub>
+       <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Project">Project Description</a></sub>
        <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Project/Details">Project Details</a></sub>
        <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Project/Materials">Materials & Methods</a></sub>
-       <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Project/Experiments">The Experiments</a></sub>
+       <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Project/Experiments" style="color: #FFF;">The Experiments</a></sub>
        <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Project/Results">Results</a></sub>
        <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Project/Data">Data analysis</a></sub>
        <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Project/HP">Human Practices</a></sub>
        <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Project/Conclusions">Conclusions</a></sub>
+    </ul></td>
-    </ul></td>
      <td><img src="images/spacer.gif" width="1" height="1" alt=""></td>
    </tr>
@@ Line 65: / Line 65: @@
      <td><img src="images/spacer.gif" width="1" height="3" alt=""></td>
    </tr>
- <td colspan="3" align="left" valign="top"><ul>
+  <tr>
+    <td colspan="3" align="left" valign="top"><ul>
        <sub2></sub2><sub2></sub2><sub2></sub2><sub2></sub2><sub2></sub2><sub2></sub2>
        <sub2></sub2><sub2></sub2><sub2></sub2><sub2></sub2><sub2></sub2><sub2></sub2>
@@ Line 72: / Line 73: @@
        <sub2><a href="#One" style="color: #FFF;">Courses</a></sub2>
        <sub2><a href="#Two" style="color: #FFF;">Talks</a></sub2>
-       <sub2><a href="#Three" style="color: #FFF;">GLP's Manual</a></sub2>
+       <sub2><a href="#Three" style="color: #FFF;">DNA Extraction</a></sub2>
-       <sub2><a href="#Four" style="color: #FFF;">Registry Manual</a></sub2>
+       <sub2><a href="#Four" style="color: #FFF;">Manuals</a></sub2>
-      <sub2><a href="#Five" style="color: #FFF;">How To Create Your Team Manual</a></sub2>
-      <sub2><a href="#Five" style="color: #FFF;">DNA extraction</a></sub2>
      </ul></td>
-  <tr>
      <td><img src="images/spacer.gif" width="1" height="28" alt=""></td>
    </tr>
@@ Line 85: / Line 84: @@
 <table width="100%" border="0" id="ContenidoSecciones">
 <div style="background-color: #f3f3e2; style="width:95%">
+<!--INICIO CONTENIDO-->
 <br>
+<h2>The Experiments</h2>
+      <p>If you choose to create a model during your project, please write about it here. Modeling is not an essential part of iGEM, but we encourage any and all teams to model some aspect of their project. See previous "Best Model" awards for more information.<br><br></p>
-<!--INICIO CONTENIDO-->
+<a name="One"><h2>Experiment One</h2></a>
+      <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vivamus fringilla porta nisi sed dictum. Aliquam at rutrum nisl. Ut eros quam, condimentum sed pretium et, euismod vitae tellus. Curabitur eu blandit massa. Vestibulum at euismod purus. Sed quis pretium ligula. Cras non tristique nulla. Nunc finibus risus non purus malesuada viverra. Cras bibendum augue eu lorem faucibus, nec auctor mi iaculis. Vivamus sagittis, mi non gravida accumsan, odio dui pellentesque leo, a varius dui mauris eu diam. Maecenas laoreet tellus sed porta efficitur.<br><br></p>
-<h2>Project Description</h2>
+<a name="Two"><h2>Experiment Two</h2></a>
+      <p>Etiam tempus mi pulvinar purus iaculis bibendum. Vivamus vel risus eu enim volutpat finibus. Nullam bibendum est sit amet arcu lobortis, id laoreet ex vestibulum. Curabitur fringilla eleifend lacus, nec ornare nibh imperdiet sed. Maecenas vel velit consectetur, tempus libero quis, consectetur ex. Nulla porttitor pharetra velit. Curabitur tristique, dolor ut sodales euismod, diam diam ultricies arcu, at tincidunt tellus neque in felis. Etiam ut tempor ligula. Sed at dui sapien.<br><br></p>
-<img src="https://static.igem.org/mediawiki/2014/b/b6/Imagen.jpg" align="left" width="250" height="250" hspace="10" BORDER=10>
+<a name="Three"><h2>Experiment Three</h2></a>
+      <p>Proin aliquam nibh id elementum pellentesque. Suspendisse mollis est ut felis sagittis mollis. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Etiam accumsan ex ante, quis lobortis erat fermentum ac. Sed et egestas libero. Donec id diam vitae leo consequat interdum. Ut in sem in quam pretium finibus vitae non lectus.<br><br></p>
-<p> Cardiovascular disease has been identified as one of the leading causes of death worldwide, and particularly in North America. Cardiovascular disease is strongly associated with atherosclerotic plaque: the accumulation of oxidized lipids and foam cells over the inner layer of arteries; atherosclerotic plaque does not only affect the behaviour of factors as blood pressure, but is also a major cause of strokes and cardiovascular events. <br><br>
+<a name="Four"><h2>Experiment Four</h2></a>
-Formerly, atherosclerosis was strongly associated with the daily intake of cholesterol in products derived from an animal source; however, it has recently been shown that it is not cholesterol itself, but its oxidized derivatives which cause the development of atherosclerotic plaque. When cholesterol travels through the bloodstream, in the form of lipoproteins, it is susceptible to a variety of chemical reactions, among which oxidation caused by reactive oxygen species (ROS) is quite common. When cholesterol is oxidized, it can no longer undergo a normal metabolic control: it starts accumulating and the cells responsible for its degradation, the macrophages, are unable to metabolize it. This is the ultimate cause of Atherosclerosis. <br><br>
+      <p>Curabitur sed erat ante. Maecenas viverra odio sit amet ultrices facilisis. Nullam lobortis mi quam, sit amet placerat erat eleifend eget. Mauris commodo sem tortor, quis blandit orci laoreet vel. In hac habitasse platea dictumst. Curabitur molestie eu dolor ac bibendum. Duis fermentum tellus et dui dapibus, sed feugiat dolor sollicitudin. Nulla malesuada, tortor quis blandit dictum, odio risus rutrum sapien, varius pharetra dui tellus sed lectus. Sed eget magna at justo tempus hendrerit. Nulla in odio nec libero maximus finibus.<br><br></p>
-A variety of oxidized cholesterol species exists, it is the purpose of this project to enhance the macrophage-mediated degradation of a particular molecule, namely 7-ketocholesterol. In order to perform this task, the genes encoding three microbial enzymes will be transformed into human macrophages. The enzymes were firstly isolated from two genres of bacteria which inhabit the soil, where they are exposed to death animal rests; this kind of organisms are then naturally able to metabolize the compounds accumulated in the bodies, as oxidized cholesterol. First, we are testing the functionality of the three recombinant enzymes in E. coli and the degradation rates showed on 7-ketocholesterol, after that we will be able to transform mammalian cells. <br><br>
-By using this approach, it is expected that the ability of macrophages to degrade 7-ketocholesterol will be increased at a genomic and metabolic level. Once suitable results are obtained, a new approach will be taken so that a therapy can be developed.
-</p>
-<br>
-<h2>References </h2>
-<p>
-. Benoit C, Drouot S, Barrail-Tran A, Taburet AM. Drug-drug interactions between HMG-CoA reductase inhibitors (statins) and antiviral protease inhibitors. Clin Pharmacokinet. 2013 May 24; 52: 815-831. <br><br>
-. Biasucci LM, Biasillo G, Stefanelli A. Inflammatory markers, cholestreol and sta<br>tins: pathophysiological role and clinical importance. Cliln Chem Lab Med. 2010 Sep 27; 48(12): 1685-1691. <br>
-. Lyons MA, Brown AJ. Molecules in focus: 7-ketocholesterol. Int J Biochem Cell Biol. 1999; 31: 369-375.<br><br>
-. Mathieu JM, Mohn WW, Eltis LD, LeBlanc JC, Stewart GR, Dresen C, et al. 7-Ketocholesterol catabolism by Rhodococcus jostti RHA1. Appl Environ Microbiol. 2009 Oct 26; 76(1): 352-355. <br><br>
-. Mathieu JM, Schloendron J, Rittmann BE, Álvarez PJJ. Microbial degradation of 7-ketocholesterol. Biodegradation. 2008; 19: 807-813. <br><br>
-. Mathieu JM. Strategies for the mitigation of oxysterol-induced cytotoxicity. Texas: Rice University; 2011. <br>
-. Pérez Guerra Y. Oxidación de las LDL (lipoproteínas de baja densidad) y su relación con la patogénesis de la aterosclerosis. Revista CENIC de ciencias biológicas. 2007; 38(1): 3-11. <br><br>
-. Tubbs RS, Blouir MC, Romeo AK, Mortazavi MM, Choen-Gadol AA. Spinal cord ischemia and atherosclerosis: a review of literature. Br J Neurosurg. 2011 Dec 25; 25(6): 666-670.
- </p>
+<br><br><br>
-<br><br>
 <!--FIN CONTENIDO-->