Team:Evry/Notebook/Sensing/07-29-2014

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<img src="https://static.igem.org/mediawiki/2014/9/96/Pouet2.jpg" alt="pouet" /> <br>
<img src="https://static.igem.org/mediawiki/2014/9/96/Pouet2.jpg" alt="pouet" /> <br>
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1. Serial dilutions of compound was made from 10(-2) mol/L to 10(-8) mol/L <br>
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1. Serial dilutions of compound were made <br>
<li>Lead <br>
<li>Lead <br>

Latest revision as of 10:32, 8 September 2014

Picture

Survivability in molecules which are going to be sensed:
Ranges of concentrations of molecules that we want to sense were made as described in following tables:
pouet
1. Serial dilutions of compound were made

  • Lead
    text to print if image not found
  • Cadmium
    text to print if image not found
  • Nitrite
    text to print if image not found

    2. 300µL of E.coli BL21 was added in each eppendorf tube.
    We had 2 control tubes:
    *A negative control: 2,7mL of MB medium + 300µL of compound
    *A positive control: 2,7mL of MB medium + 300µL of E.coli BL21

    3. Incubation of tubes at 30°C overnight, 200rpm

    Jul 29

    • Retrieved from "http://2014.igem.org/Team:Evry/Notebook/Sensing/07-29-2014"