Team:UCL/experiments

From 2014.igem.org

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<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></div>
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></div>
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<p>After successfully transforming the <i>B. subtilis</i> and <i>P. aeruginosa</i> azo-reductase plasmids into competent <i>E. coli</i> NEB5lpha cells we then performed a diagnostic digest and gel electrophoresis experiment to ascertain that these plasmids contained the gene we expected.  Each plasmid was digested using two restriction enzymes chosen to digest DNA as specific points on the plasmids and create fragments of known length which we could then confirm using gel electrophoresis.</p>
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<p>After successfully transforming these plasmids into competent <i>E. coli</i> NEB5alpha cells we then performed a diagnostic digest and gel electrophoresis experiment to ascertain that these plasmids contained the gene we expected.  Each plasmid was digested using two restriction enzymes chosen to digest DNA as specific points on the plasmids and create fragments of known length which we could then confirm using gel electrophoresis.</p>
<h4>Creation of azo-reductase BioBrick parts from plasmids</h4>
<h4>Creation of azo-reductase BioBrick parts from plasmids</h4>

Revision as of 17:34, 6 September 2014

Goodbye Azo Dye : iGEM 2014 - University College London

 

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