Team:TCU Taiwan/Project

From 2014.igem.org

(Difference between revisions)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
{{CSS/Main}}
-
{{CSS/Main}}
+
<!--html>
-
 
+
-
<html>
+
<head>
<head>
<meta name="google-site-verification" content="pY8JgIYK6zMi9LoymHQcUKbj-xnD-rcPBrYI-xllU9o" />
<meta name="google-site-verification" content="pY8JgIYK6zMi9LoymHQcUKbj-xnD-rcPBrYI-xllU9o" />
</head>
</head>
 +
<body style="background:#ffffff">
</html>
</html>
 +
<html>
-
<html>
 
<!--Analytics-->
<!--Analytics-->
<script>
<script>
Line 37: Line 36:
   #jamboreemenu  li:hover li a:hover {background: #A66B38; width:150px;}
   #jamboreemenu  li:hover li a:hover {background: #A66B38; width:150px;}
   #drop-nav li ul li {border-top: 10px; }
   #drop-nav li ul li {border-top: 10px; }
-
   #gotop {
+
    
 +
#gotop {
     display: none;
     display: none;
     position: fixed;
     position: fixed;
Line 48: Line 48:
     cursor: pointer;
     cursor: pointer;
}
}
 +
</style>
-
fieldset {
+
 
-
border:0;
+
<!--End Analytics-->
-
padding:10px;
+
<!-- this section changes the default wiki template to a white full width background -->
-
margin-bottom:10px;
+
 
-
background:#EEE;
+
<style type="text/css">
-
}
+
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
-
legend {
+
 
-
padding:5px 10px;
+
#top-section { /*-- styling for default menu bar (edit, page, history, etc.) --*/
-
background-color:#0092C7;
+
-
color:#FFF;
+
border: 0 none;
-
}
+
height: 14px;
 +
z-index: 100;
 +
top: 0;
 +
position: fixed;
 +
width: 975px;
 +
left: 50%;
 +
margin-left: -487px;
 +
}
 +
 
 +
#globalWrapper, #content { /*-- changes default wiki settings --*/
 +
width: 100%;
 +
height: 100%;
 +
border: 0px;
 +
background-color: transparent;
 +
margin: 0px;
 +
padding: 0px;
 +
}
 +
 
 +
html, body, .wrapper { /*-- changes default wiki settings --*/
 +
width: 100%;
 +
height: 100%;
 +
background-color: transparent;
 +
}
</style>
</style>
<script type="text/javascript">
<script type="text/javascript">
Line 80: Line 103:
<script type="text/javascript"><!--  
<script type="text/javascript"><!--  
Image0 = new Image(100,50); Image0.src = "https://static.igem.org/mediawiki/2014/a/aa/TCU-Contact-bot1.png";
Image0 = new Image(100,50); Image0.src = "https://static.igem.org/mediawiki/2014/a/aa/TCU-Contact-bot1.png";
-
Image1 = new Image(100,50); Image1.src = "https://static.igem.org/mediawiki/2014/1/10/TCU_TEACH.jpg";
 
-
Image2 = new Image(100,50); Image2.src = "https://static.igem.org/mediawiki/2014/f/f8/2013_TCUIGEM.png";
 
-
Image3 = new Image(100,50); Image3.src = "https://static.igem.org/mediawiki/2014/4/47/TCU_Moe_logo.png";
 
-
Image4 = new Image(100,50); Image4.src = "https://static.igem.org/mediawiki/2014/b/b1/TCU_SUP_LOGO.png";
 
-
Image5 = new Image(100,50); Image5.src = "https://static.igem.org/mediawiki/2014/a/ae/TZUCHI_SUP.jpg";
 
// --></script>
// --></script>
-
<!--End Analytics-->
+
<!-- here ends the section that changes the default wiki template to a white full width background -->
-
<body style="background:#ffffff">
+
-
<table width="100%" align="center" style="background:#ffffff" border="0">
+
-
<tr>  
+
-
<!--navigation menu -->
 
-
<td align="center" colspan="3">
 
 +
<!-- beginning of your page -->
<table width="100%" border="0" style="background-color:#F2DE79" height="150px">
<table width="100%" border="0" style="background-color:#F2DE79" height="150px">
   <tr>
   <tr>
     <td width="0%">&nbsp;</td>
     <td width="0%">&nbsp;</td>
-
     <td width="128" height="128" align="left" valign="middle"><img src="https://static.igem.org/mediawiki/2014/5/51/TCU-Attributions-logo.png" width="128" height="128"></td>
+
     <td width="128" height="128" align="left" valign="middle"><img src="https://static.igem.org/mediawiki/2014/f/f4/TCU-Project-logo.png" width="128" height="128"></td>
-
     <td width="68%" align="left" valign="middle"><h6><font face="Trebuchet MS" size="7" color="#484644">Attributions</font></h6></td>
+
     <td width="68%" align="left" valign="middle"><h6><font face="Trebuchet MS" size="7" color="#484644">Project</font></h6></td>
     <td width="19%" align="center"><img src="https://static.igem.org/mediawiki/2014/c/c2/TCU-Logo-up.png" width="90%"></td>
     <td width="19%" align="center"><img src="https://static.igem.org/mediawiki/2014/c/c2/TCU-Logo-up.png" width="90%"></td>
     <td width="0%">&nbsp;</td>
     <td width="0%">&nbsp;</td>
   </tr>
   </tr>
</table>
</table>
-
<!--end navigation menu -->
 
-
 
<table id="jamboreemenu" width="100%" cellspacing="0" align="center">
<table id="jamboreemenu" width="100%" cellspacing="0" align="center">
Line 136: Line 148:
<td align="center">
<td align="center">
<ul id="drop-nav">
<ul id="drop-nav">
-
   <li><a href="#" style="cursor:default"><font size="5">Project</font></a>
+
   <li><a href="#" style="cursor:default"><font size="5" style="color:#DBAC52">Project</font></a>
     <ul>
     <ul>
-
       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Project"><font size="3">Project</font></a></li>
+
       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Project" style="color:#DBAC52"><font size="3">Project</font></a></li>
       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Parts"><font size="3">Parts</font></a></li>
       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Parts"><font size="3">Parts</font></a></li>
       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics"><font size="3">Human Pratics</font></a></li>
       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics"><font size="3">Human Pratics</font></a></li>
Line 157: Line 169:
<td align="center">
<td align="center">
<ul id="drop-nav">
<ul id="drop-nav">
-
   <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Safety" ><font size="5">Safety</font></a></li>
+
   <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Safety"><font size="5">Safety</font></a></li>
</ul>
</ul>
</td>
</td>
Line 170: Line 182:
<td align="center">
<td align="center">
<ul id="drop-nav">
<ul id="drop-nav">
-
<li><a href="https://2014.igem.org/Team:TCU_Taiwan/Attributions" style="color:#DBAC52"><font size="5">Attributions</font></a></li>
+
<li><a href="https://2014.igem.org/Team:TCU_Taiwan/Attributions"><font size="5">Attributions</font></a></li>
</ul>
</ul>
</td>
</td>
Line 180: Line 192:
</td>
</td>
</tbody></table>
</tbody></table>
 +
<div id="contentcontainer">
 +
<!-- this table contains the navigation bar --><!--navigation menu -->
 +
<td align="center" colspan="3">
-
<tr><td colspan="3" height="1px" background="#F3E4C2"><img src="https://static.igem.org/mediawiki/2014/c/c1/TCU-Line.png" width="100%">
+
</td>  
 +
</tr>
</tbody></table>
</tbody></table>
 +
<tr><td colspan="3" height="1px" background="#F3E4C2"><img src="https://static.igem.org/mediawiki/2014/c/c1/TCU-Line.png" width="100%"></tr> </td></tr>
 +
<!--end navigation menu --><!-- end of navigation bar -->
-
<!--main content -->
 
-
<table width="70%" align="center" style="background:#ffffff">
 
 +
<!-- Page content begins -->
-
<!--welcome box -->
+
<table width="975px" align="center">
 +
<tr>
 +
  <td height="50px">&nbsp;</td>
 +
  <td>&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td style="background-color:#FFF2B5" height="20px"><font face="Trebuchet MS" size="7" color="#A66B38">Introduction</font></td>
 +
  <td>&nbsp;</td>
 +
</tr>
 +
<tr>
-
<!-- end welcome box -->
+
<td width="50%" align="center"><img src="https://static.igem.org/mediawiki/2014/c/c2/TCU-Logo-up.png" width="80%"></td>
-
+
<td width="50%">
 +
<table width="50%" align="center">
 +
<tr ><td coslpan="3" height="35px"><img src="https://static.igem.org/mediawiki/2014/c/cc/TCU-Anti.png " width="30%"/></td></tr>
 +
<tr>
 +
<td style="border:0px solid black;" align="center" width="33%" height ="35px"
 +
onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B> 
 +
<a href="#abstract"
 +
style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Abstract</font></a> </td>
 +
</tr>
 +
<tr>
 +
<td style="border:0px solid black;" align="center"  width="33%" height ="35px"
 +
onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B> 
 +
<a href="#topic1"
 +
style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Topic 1</font></a> </td>
 +
</tr>
<tr>
<tr>
-
  <td colspan="3"  height="30px"></td>
+
<td style="border:0px solid black;" align="centerwidth="33%" height ="35px"  
 +
onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B> 
 +
<a href="#topic2"
 +
style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Topic 2</font></a> </td>
</tr>
</tr>
-
<tr> <td colspan="3"  height="15px">
+
 
-
<table width="70%" border="0" cellspacing="0" cellpadding="0" align="center">
+
-
  <tr>
+
-
    <td align="justify"><font size="5" face="Verdana" color="#D92332">We would like to thank all the people who came to our progress presentations and gave useful feedback.</font></td>
+
-
  </tr>
+
-
</table></td></tr>
+
<tr>
<tr>
-
  <td colspan="3"  height="15px">
+
<td style="border:0px solid black;" align="center" width="33%" height ="35px"  
-
  <table width="100%" border="0" cellspacing="0" cellpadding="0" align="center">
+
onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B>
-
        <tr>
+
<a href="#topic3"
-
          <td height="30px">&nbsp;</td>
+
style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Topic 3</font></a> </td>
-
        </tr>
+
</tr>
-
        <tr>
+
-
          <td>
+
-
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
+
-
            <tr>
+
-
              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Experiment</font></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td height="20px" colspan="2">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td colspan="2"><font size="3" face="Verdana" color="#0092C7">Bobo, Regina, Peiyi, Eating</font>
+
-
</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td colspan="2" height="20px">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td colspan="2"><font face="Trebuchet MS" size="5" color="#90B849">Contributions</font></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td colspan="2" height="10px">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td width="50%" valign="top"><font size="3" face="Verdana" color="#333"><li>Transformation ( Cas9 in  pSB1C3 into DH5α)<br>
+
-
                <li>Extract plasmid with pSB1C3  from DH5α.<br>
+
-
                <li>Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011.<br>
+
-
                <li>Use Enzyme digestion (<em>PstI</em> and <em>EcoRI</em>) to confirm whether pSB1C3 and pBluescript is successfully  extracted.<br>
+
-
                <li>Purification of RFP sequence from pSB1C3 Prepare Cm. plate、LB. plate.<br>
+
-
                <li>Ligation of RFP and pBluescript SK(-).<br>
+
-
                <li>Transformation clone RFP/pBluescript SK(-) to DH5α.<br>
+
-
                <li>Extract plasmid clone RFP/pBluescript SK(-).</li></font></td>
+
-
              <td rowspan="3">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td>
+
-
              <fieldset>
+
-
<legend><font face="Trebuchet MS" size="4" color="#fff">Experiment Design</font></legend>
+
-
                <p><font size="3" face="Verdana" color="#90B849">Ted and                  Bobo </font><font size="3" face="Verdana" color="#90B849"><br><br>
+
-
                </font><font size="3" face="Verdana" color="#333">
+
-
Design gRNA for AmpR.
+
-
                </font></p>
+
-
</fieldset>
+
-
</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td><fieldset>
+
-
<legend><font face="Trebuchet MS" size="4" color="#fff">Biobricks Design</font></legend>
+
-
                <p><font size="3" face="Verdana" color="#90B849">Ted</font><font size="3" face="Verdana" color="#90B849"><br><br>
+
-
                </font><font size="3" face="Verdana" color="#333">
+
-
Design the gRNA for different resistance gene.
+
-
                </font></p>
+
-
</fieldset></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td height="30px">&nbsp;</td>
+
-
              <td>&nbsp;</td>
+
-
            </tr>
+
-
          </table></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td> <table width="100%" border="0" cellspacing="0" cellpadding="0">
+
-
            <tr>
+
-
              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Modeling</font></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td height="10px" colspan="2">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td colspan="2"><font size="3" face="Verdana" color="#0092C7">House, Bobo, Chao-Di Chang(National Chiao Tung University)</font></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td height="10px" colspan="2">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td colspan="2"><font face="Trebuchet MS" size="5" color="#90B849">Contributions</font></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td colspan="2" height="10px">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td width="50%" valign="top"><font size="3" face="Verdana" color="#333"><li>Infectional efficiency ratio of  M13KO7.<br>
+
-
                <li> Release titer.(Releasing titer of phagemid which is E.coli infected by helper phage)<br>
+
-
                </font></td>
+
-
              <td rowspan="3">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td>
+
-
           
+
-
</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td height="30px">&nbsp;</td>
+
-
              <td>&nbsp;</td>
+
-
            </tr>
+
-
          </table></td>
+
-
        </tr>
+
-
    </table>
+
<tr>
-
    <table width="100%" border="0" cellspacing="0" cellpadding="0">
+
<td style="border:0px solid black;" align="center" width="33%" height ="35px"  
-
            <tr>
+
onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B>
-
              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Human Practice</font></td>
+
<a href="#references"
-
            </tr>
+
style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">References</font></a> </td>
-
            <tr>
+
</tr>
-
              <td height="20px" colspan="2">&nbsp;</td>
+
<tr>
-
            </tr>
+
  <td align="right" height ="35px"><img src="https://static.igem.org/mediawiki/2014/b/b8/TCU-Phage.png" width="30%"/></td>
-
            <tr>
+
</tr>
-
              <td colspan="2"><font size="3" face="Verdana" color="#0092C7">Eating</font>
+
-
</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td colspan="2" height="20px">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td colspan="2"><font face="Trebuchet MS" size="5" color="#90B849">Contributions</font></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td colspan="2" height="10px">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td width="50%" valign="top"><font size="3" face="Verdana" color="#333"><li>Held the 2014 Synthetic Biology information meeting of local senior high school and Tzu Chi University as a chief convener.<br>
+
-
                <li>Contact with National Chiao Tung University for cooperation.<br></font></td>
+
-
              <td rowspan="3">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td>
+
-
             
+
-
</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td height="30px">&nbsp;</td>
+
-
              <td>&nbsp;</td>
+
-
            </tr>
+
-
          </table>
+
-
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
+
-
            <tr>
+
-
              <td bgcolor="#FFF2B5" height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Wiki</font></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td height="20px" colspan="2">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td colspan="2"><font size="3" face="Verdana" color="#0092C7">House</font>
+
-
</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td colspan="2" height="20px">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td colspan="2"><font face="Trebuchet MS" size="5" color="#90B849">Contributions</font></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td colspan="2" height="10px">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td width="50%" valign="top"><font size="3" face="Verdana" color="#333"><li>Construct the wiki page.<br>
+
-
                <li>Coding,coding and coding the wiki page.<br> <li>Study all about Synthetic Biology from wetlab to drylab.</font></td>
+
-
              <td rowspan="3">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td>
+
-
             
+
-
</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td height="30px">&nbsp;</td>
+
-
              <td>&nbsp;</td>
+
-
            </tr>
+
-
          </table>
+
-
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
+
-
            <tr>
+
-
              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">And last but not least</font></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td height="20px" colspan="2">
+
-
              <table width="90%" border="0" cellspacing="0" cellpadding="0" align="center">
+
-
                <tr>
+
-
                  <td width="48%">
+
-
                  <fieldset>
+
-
<legend><font face="Trebuchet MS" size="4" color="#fff">Art Design</font></legend>
+
-
                <p><font size="3" face="Verdana" color="#90B849">Peiyi, Regina, Eating, House</font><font size="3" face="Verdana" color="#90B849"><br><br>
+
-
                </font><font size="3" face="Verdana" color="#333">Design of poster and PPT.
+
-
<br>
+
-
Design of team uniform.
+
-
 
+
-
                </font></p>
+
-
</fieldset>
+
-
                </td>
+
-
                  <td width="10px">&nbsp;</td>
+
-
                  <td>
+
-
                    <fieldset>
+
-
                      <legend><font face="Trebuchet MS" size="4" color="#fff">Photo & Video</font></legend>
+
-
                      <p><font size="3" face="Verdana" color="#90B849">Sharon, Eating</font><font size="3" face="Verdana" color="#90B849"><br><br>
+
-
                        </font><font size="3" face="Verdana" color="#333"> Take photo and video.
+
-
  <br>Arrange all photos.
+
-
                         
+
-
                        </font></p>
+
-
                      </fieldset>
+
-
                  </td>
+
-
                  </tr>
+
-
                <tr>
+
-
                  <td><fieldset>
+
-
                      <legend><font face="Trebuchet MS" size="4" color="#fff">Administeration</font></legend>
+
-
                      <p><font size="3" face="Verdana" color="#90B849">Eating</font><font size="3" face="Verdana" color="#90B849"><br><br>
+
-
                        </font><font size="3" face="Verdana" color="#333">Apply for the allowances.<br>Arrange all files.</font></p>
+
-
                      </fieldset></td>
+
-
                  <td>&nbsp;</td>
+
-
                  <td></td>
+
-
                </tr>
+
-
              </table></td>
+
-
            </tr>
+
-
            <tr>
+
</table>
-
              <td width="50%" colspan="2"></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td>
+
-
             
+
</td>
</td>
-
            </tr>
 
-
            <tr>
 
-
              <td></td>
 
-
            </tr>
 
-
            <tr>
 
-
              <td height="30px">&nbsp;</td>
 
-
              <td>&nbsp;</td>
 
-
            </tr>
 
-
          </table>
 
-
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
 
-
            <tr>
 
-
              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Video support</font></td>
 
-
            </tr>
 
-
            <tr>
 
-
              <td height="20px" colspan="2">&nbsp;</td>
 
-
            </tr>
 
-
            <tr>
 
-
              <td colspan="2"><font size="3" face="Verdana" color="#0092C7">Wang, Yiru and Chen, Jiawei</font>
 
-
</td>
 
-
            </tr>
 
-
            <tr>
 
-
              <td colspan="2" height="20px">&nbsp;</td>
 
-
            </tr>
 
-
            <tr>
 
-
              <td colspan="2"><font face="Trebuchet MS" size="3" color="#90B849">we should be obliged to them for their assistance in this matter.</font></td>
 
-
            </tr>
 
-
            <tr>
 
-
              <td colspan="2" height="10px">&nbsp;</td>
 
-
            </tr>
 
-
            <tr>
 
-
              <td width="50%" align="center"><p><img src="https://static.igem.org/mediawiki/2014/6/66/TCU_Yiru.jpg" width="50%"></p>
 
-
              <p><font size="3" face="Verdana" color="#333">Wang, Yiru</font></p></td>
 
-
              <td rowspan="3" align="center"><p><img src="https://static.igem.org/mediawiki/2014/7/75/TCU_Jiawei.jpg" width="50%"></p>
 
-
              <p><font size="3" face="Verdana" color="#333">Chen, Jiawei</font></p></td>
 
-
            </tr>
 
-
            <tr>
 
-
              <td>
 
-
             
 
-
</td>
 
-
            </tr>
 
-
            <tr>
 
-
              <td></td>
 
-
            </tr>
 
-
            <tr>
 
-
              <td height="30px">&nbsp;</td>
 
-
              <td>&nbsp;</td>
 
-
            </tr>
 
-
          </table>
 
-
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
 
-
            <tr>
 
-
              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Special Thanks</font></td>
 
-
            </tr>
 
-
            <tr>
 
-
              <td height="20px" colspan="2">&nbsp;</td>
 
-
            </tr>
 
-
            <tr>
 
-
              <td colspan="2"><font size="3" face="Verdana" color="#0092C7">Our partner : <a style="text-decoration: none; color:#0092C7" href="https://2014.igem.org/Team:NCTU_Formosa" target="_blank">NCTU_Formosa</a></font>
 
-
</td>
 
-
            </tr>
 
-
            <tr>
+
</tr>
-
              <td colspan="2" height="10px">&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td width="50%" valign="top"><font size="3" face="Verdana" color="#333">
+
-
                <li>We are extremely appreciative of NCTU_Formosa that helped and support us.<br>
+
-
                <li>Modeling courtesy of <a href="https://2014.igem.org/Team:NCTU_Formosa/team#CHAO-DI_CHANG" target="_blank">Chao-Di Chang.</a><img src="https://static.igem.org/mediawiki/2014/7/7f/TCU_HP_NJTU.jpg" width="70%"><br></font></td>
+
-
              <td rowspan="3" align="center"><a href="https://2014.igem.org/Team:NCTU_Formosa" target="_blank" title="NCTU_Formosa Wiki"><img src="https://static.igem.org/mediawiki/2014/0/09/FINAL.gif" width="50%"></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td>
+
-
             
+
-
</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td height="30px">&nbsp;</td>
+
-
              <td>&nbsp;</td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Sponsors</font></td>
+
-
            </tr>
+
-
            <tr>
+
-
              <td height="10px" colspan="2">&nbsp;</td>
+
-
            </tr>
+
-
          </table>
+
-
         
+
-
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
+
-
  <tr>
+
-
    <td align="center">
+
-
   
+
-
    <!--3-->
+
-
    <a href="http://english.moe.gov.tw/" title="Ministry of Education,Republic of China" target="_blank"><img src="https://static.igem.org/mediawiki/2014/8/83/TCU_Moe_logo_BLACK.png" height="100" onMouseMove="this.src=Image3.src" onMouseOut="this.src='https://static.igem.org/mediawiki/2014/8/83/TCU_Moe_logo_BLACK.png'"></a>&nbsp;&nbsp;&nbsp;
+
-
   
+
-
    <!--1-->
+
-
    <a href="http://tec.tcu.edu.tw/100/" title="Center of Teaching Excellence,TCU" target="_blank"><img src="https://static.igem.org/mediawiki/2014/2/26/TCU_TEACH_BLACK.png" height="100" onMouseMove="this.src=Image1.src" onMouseOut="this.src='https://static.igem.org/mediawiki/2014/2/26/TCU_TEACH_BLACK.png'"></a>&nbsp;&nbsp;
+
-
   
+
-
    <!--5-->
+
-
    <a href="http://www.tzuchi.org/" title="Buddhist Compassion Relief Tzu Chi Foundation" target="_blank"><img src="https://static.igem.org/mediawiki/2014/f/ff/TZUCHI_SUP_BLACK.png" height="90" onMouseMove="this.src=Image5.src" onMouseOut="this.src='https://static.igem.org/mediawiki/2014/f/ff/TZUCHI_SUP_BLACK.png'"></a>&nbsp;&nbsp;
+
-
   
+
-
    <!--4-->
+
-
    <a href="http://eng.tcu.edu.tw/" title="Tzu Chi University" target="_blank"><img src="https://static.igem.org/mediawiki/2014/4/43/TCU_SUP_LOGO_BLACK.png" height="90" onMouseMove="this.src=Image4.src" onMouseOut="this.src='https://static.igem.org/mediawiki/2014/4/43/TCU_SUP_LOGO_BLACK.png'"></a>&nbsp;
+
-
 
+
-
    <!--2-->
+
-
    <a href="https://2013.igem.org/Team:TzuChiU_Formosa" title="2013 TzuChiU_Formosa" target="_blank"><img src="https://static.igem.org/mediawiki/2014/7/75/2013_TCUIGEM_BLACK.png" height="130" onMouseMove="this.src=Image2.src" onMouseOut="this.src='https://static.igem.org/mediawiki/2014/7/75/2013_TCUIGEM_BLACK.png'"></a>
+
-
   
+
-
   
+
-
   
+
-
   
+
-
   
+
-
  </td>
+
-
  </tr>
+
</table>
</table>
-
<table width="100%" border="0" cellspacing="0" cellpadding="0">
 
-
            <tr>
 
-
              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">General support</font></td>
 
-
            </tr>
 
-
            <tr>
 
-
              <td height="20px" colspan="2">&nbsp;</td>
 
-
            </tr>
 
-
            <tr>
+
 
-
              <td colspan="2" height="10px">
+
 
-
              <table width="600" height="90" border="0" cellspacing="0" cellpadding="0" align="left"style="background-color:#01395C">
+
<!-- Abstract Section-->
-
   <tr>
+
<table width="975px" align="center">
-
    <td><a href="http://www.microbiology.tcu.edu.tw/english/home.html" title="Master program in Microbiology and Immunology" target="_blank"><img src="http://www.microbiology.tcu.edu.tw/images/logo/logo_v2.png" width="600" height="90"></a>
+
<tr ><td coslpan="3" height="35px"></td></tr>
-
    </td>
+
 
-
  </tr>
+
<tr><td colspan="3" style="background-color:#FFF2B5" height="20px"> <font face="Trebuchet MS" size="7" color="#A66B38" id="abstract">Abstract</font> </td> </tr>
-
</table>
+
<tr>
 +
   <td valign="top" height="10px">&nbsp;</td>
 +
  <td ></td>
 +
  <td valign="top">&nbsp;</td>
 +
<tr>
 +
<td width="42%" valign="top">  
 +
<font size="3" face="Verdana" color="#333"><p>To access this, we use a special <a href="http://en.wikipedia.org/wiki/Phagemid" target="_blank">phagemid</a>  for phage display. The phagemid contains a additional phage replication origin.  When helper phage (a phage whose own ori is knock-out) infection take place,  phagemid can be packaged as genome for progeny. Since we put an <a href="http://en.wikipedia.org/wiki/Antibiotic_resistance" target="_blank">antibiotic</a> resistance gene targeting <a href="http://en.wikipedia.org/wiki/CRISPR" target="_blank">CRISPR</a> system on our phagemid, this phage display can  spread our CRISPR among bacterium nearby and remove their resistance  efficiently.</p></font>
</td>
</td>
-
            </tr>
+
 
-
            <tr>
+
<td width="5%" > </td>
-
              <td colspan="2" height="15px">&nbsp;</td>
+
 
-
            </tr>
+
 
-
            <tr>
+
<td width="42%" valign="top">  
-
              <td colspan="2" height="10px">
+
<font size="3" face="Verdana" color="#333"><p>Trogene Horse is a new concept combining  phage therapy with CRISPR, and this combination can solve both safety  concern(phage therapy) and efficiency problem(CRISPR). We believe our project  can deal with bacterial resistance problem on agriculture, and hope it can  access on medicine one day.</p></font></td>
-
              <table width="600" height="90" border="0" cellspacing="0" cellpadding="0" align="left"style="background-color:#000000">
+
 
-
  <tr>
+
 
-
    <td><a href="http://www.mi.tcu.edu.tw/english_web/introduction.htm" title="Department of Medical Informatics" target="_blank"><img src="http://www.mitest.tcu.edu.tw/images/logo/logo_v3.png" width="550" height="100"></a>
+
<tr ><td coslpan="2" height="35px"></td></tr>
-
    </td>
+
 
-
  </tr>
+
</tr>
</table>
</table>
-
</td>
 
-
            </tr>
 
-
              <td colspan="2" height="15px">&nbsp;</td>
 
-
            </tr>
 
-
          <tr>
 
-
              <td colspan="2" height="10px">
 
-
              <table width="600" height="90" border="0" cellspacing="0" cellpadding="0"  align="left">
 
-
  <tr>
 
-
    <td><a href="http://www.dmbhg.tcu.edu.tw/dmbhg.html" title="Department of Molecular Biology and Human Genetics" target="_blank"><img src="http://www.dmbhg.tcu.edu.tw/image/dLogo.jpg" width="100%"></a></td>
 
-
  </tr>
 
-
</table>
 
-
</td>
 
-
            </tr>
 
-
            <tr>
 
-
              <td width="50%" height="30px">&nbsp;</td>
 
-
              <td>&nbsp;</td>
 
-
            </tr>
 
-
          </table>
 
-
    </td>
 
-
</tr>
 
-
<tr> <td colspan="3"  height="5px"> </td></tr>
 
-
<!--Attributions content -->
+
<!-- End of abstract section -->
-
<tr><td > <h3> iGEM Team attributions page</h3></td>
+
 
-
<td ></td >
+
 
-
<td > <h3> </h3></td>
+
 
-
</tr>
+
<!-- 3 columns content
 +
this area is divided intro 3 columns, you can add more information about your team, project, city or mention your sponsors! -->
 +
<table width="975px" align="center">
 +
 
 +
<tr> <td> <h3 id="topic1">Topic 1</h3> </td> </tr>
<tr>
<tr>
-
<td width="45%" valign="top" colspan="3">  
+
<!-- first column -->
 +
<td width="30%">
 +
<p>&nbsp;</p>
 +
</td>
 +
 
 +
<td width="5%"></td>
-
<p>
 
-
Each team must clearly attribute work done by the student team members on this page.  The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster.
 
-
</p>
 
 +
<!-- second column -->
 +
<td width="30%">
 +
<p>&nbsp;</p>
</td>
</td>
 +
<td width="5%"></td>
 +
<!-- third column -->
 +
<td width="30%">
 +
<img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="300px">
 +
</td>
-
<tr>
+
</tr>
-
<td > <h3> Why do we have this requirement?</h3></td>
+
</table>
-
<td></td>
+
<!-- end of 3 column content -->
-
<td > <h3>Attribution Template </h3></td>  
+
 
-
  </tr>
+
 
 +
<!-- Topic 2 -->
 +
<table width="975px" align="center">
 +
 
 +
<tr> <td> <h3 id="topic2">Topic 2</h3> </td> </tr>
<tr>
<tr>
 +
<!-- first column -->
 +
<td width="30%">
 +
<img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="300px">
 +
</td>
-
<td width="45%" valign="top">
+
<td width="5%"></td>
-
<p>
 
-
We have this requirement to help the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, just be sure to report the work your team did and the work that was done by others.
 
-
</p>
 
-
<p>
+
<!-- second column -->
-
For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is something that is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.  
+
<td width="30%">
-
</p>
+
<img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="300px">
 +
</td>
 +
<td width="5%"></td>
-
<p>
 
-
A great example of complete attribution comes from the <a href="https://2011.igem.org/Team:Imperial_College_London/Team">Imperial College London 2011 team</a> (scroll down to the bottom of their team page to see attributions).
 
-
</p>
 
-
</td>
+
<!-- third column -->
-
<td></td>
+
<td width="30%">
-
<td width="45%"  valign="top">  
+
<p>&nbsp;</p>
-
<p>
+
</td>
-
Here are some of the fields we recommend you have on this page. If there are other areas not listed below, but applicable to your team/project, please feel free to also list them on your attributions page. Please feel free to remove any areas not applicable to your project.
+
</tr>
-
</p>
+
-
<ol>
 
-
<li>General Support</li>
 
-
<li>Project support and advice</li>
+
</table>
 +
<!-- end of 3 column content -->
-
<li>Fundraising help and advice</li>
 
-
<li>Lab support</li>
 
-
<li>Difficult technique support</li>
+
<!-- 2 columns content
 +
this area is divided intro 3 columns, you can add more information about your team, project, city or mention your sponsors! -->
 +
<table width="975px" align="center">
-
<li>Project advisor support</li>
+
<tr> <td> <h3 id="topic3">Topic 3</h3>  </td> </tr>
-
<li>Wiki support</li>
+
<tr>
 +
<!-- first column -->
-
<li>Presentation coaching</li>
+
<td width="65%">
 +
<p>&nbsp;</p>
 +
</td>
-
<li>Policy & Practices support</li>
 
-
<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team.</li>
+
<td width="5%"></td>
-
</ol>
+
 
 +
<!-- second column -->
 +
<td width="30%">
 +
<img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="200px">
</td>
</td>
</tr>
</tr>
 +
 +
</table>
</table>
 +
<!-- end of 2 column content -->
 +
 +
<!-- references -->
 +
 +
<table width="975px" align="center">
 +
<tr ><td height="35px"></td></tr>
 +
 +
<tr><td> <h3 id="references">References</h3>
 +
<ul>
 +
<li></li>
 +
</ul>
 +
</td>
 +
</tr>
 +
 +
</tr></table>
<table width="100%" border="0" cellspacing="0" cellpadding="0">
<table width="100%" border="0" cellspacing="0" cellpadding="0">
Line 725: Line 453:
   </tr>
   </tr>
</table>
</table>
 +
<!==end of references -->
<div id="gotop">^</div>
<div id="gotop">^</div>
</html>
</html>

Revision as of 13:06, 14 October 2014


<script>

 (function(i,s,o,g,r,a,m){i['GoogleAnalyticsObject']=r;i[r]=i[r]||function(){
 (i[r].q=i[r].q||[]).push(arguments)},i[r].l=1*new Date();a=s.createElement(o),
 m=s.getElementsByTagName(o)[0];a.async=1;a.src=g;m.parentNode.insertBefore(a,m)
 })(window,document,'script','//www.google-analytics.com/analytics.js','ga');
 ga('create', 'UA-48805552-2', 'igem.org');
 ga('send', 'pageview');

</script>

<style type="text/css">

 #jamboreemenu ul {list-style: none;padding: 0px;margin: 0px;}
 #jamboreemenu ul li {display: block;position: relative;float: left; #A66B38; width:150px;}
   #jamboreemenu li ul {display: none;}
   #jamboreemenu ul li a {display: block;background: #A66B38;  width:150px; padding: 10px 5px 10px 10px;text-decoration: none;
          white-space: nowrap;color: #fff;}
 #jamboreemenu li a:hover {background: #F2DE79;  width:150px;}
   #jamboreemenu li:hover ul {display: block; position: absolute;}
  #jamboreemenu li:hover li {float: none;}
 #jamboreemenu  li:hover a {background: #F2DE79; width:150px;}
 #jamboreemenu  li:hover li a:hover {background: #A66B38; width:150px;}
 #drop-nav li ul li {border-top: 10px; }
 
#gotop {
   display: none;
   position: fixed;
   right: 20px;
   bottom: 20px;    
   padding: 10px 15px;    
   font-size: 20px;
   background: #777;
   color: white;
   cursor: pointer;

} </style>


<style type="text/css">

  1. contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
  1. top-section { /*-- styling for default menu bar (edit, page, history, etc.) --*/

border: 0 none; height: 14px; z-index: 100; top: 0; position: fixed; width: 975px; left: 50%; margin-left: -487px; }

  1. globalWrapper, #content { /*-- changes default wiki settings --*/

width: 100%; height: 100%; border: 0px; background-color: transparent; margin: 0px; padding: 0px; }

html, body, .wrapper { /*-- changes default wiki settings --*/ width: 100%; height: 100%; background-color: transparent; } </style> <script type="text/javascript">

$(function(){

   $("#gotop").click(function(){
       jQuery("html,body").animate({
           scrollTop:0
       },1000);
   });
   $(window).scroll(function() {
       if ( $(this).scrollTop() > 300){
           $('#gotop').fadeIn("fast");
       } else {
           $('#gotop').stop().fadeOut("fast");
       }
   });

}); </script> <script type="text/javascript"></script>


  <img src="TCU-Project-logo.png" width="128" height="128">

Contents

Project
<img src="TCU-Logo-up.png" width="90%">  
<tbody>
<tbody> </tbody>

<td align="center" colspan="3">

</td> </tr> </tbody></table> <tr><td colspan="3" height="1px" background="#F3E4C2"><img src="TCU-Line.png" width="100%"></tr> </td></tr>




   
Introduction  
<img src="TCU-Logo-up.png" width="80%">
<img src="TCU-Anti.png " width="30%"/>

<a href="#abstract"

style="color:#0B6D78; text-decoration: none;">Abstract</a>

<a href="#topic1"

style="color:#0B6D78; text-decoration: none;">Topic 1</a>

<a href="#topic2"

style="color:#0B6D78; text-decoration: none;">Topic 2</a>

<a href="#topic3"

style="color:#0B6D78; text-decoration: none;">Topic 3</a>

<a href="#references"

style="color:#0B6D78; text-decoration: none;">References</a>
<img src="TCU-Phage.png" width="30%"/>




</tr>

Abstract
   

To access this, we use a special <a href="http://en.wikipedia.org/wiki/Phagemid" target="_blank">phagemid</a> for phage display. The phagemid contains a additional phage replication origin. When helper phage (a phage whose own ori is knock-out) infection take place, phagemid can be packaged as genome for progeny. Since we put an <a href="http://en.wikipedia.org/wiki/Antibiotic_resistance" target="_blank">antibiotic</a> resistance gene targeting <a href="http://en.wikipedia.org/wiki/CRISPR" target="_blank">CRISPR</a> system on our phagemid, this phage display can spread our CRISPR among bacterium nearby and remove their resistance efficiently.

Trogene Horse is a new concept combining phage therapy with CRISPR, and this combination can solve both safety concern(phage therapy) and efficiency problem(CRISPR). We believe our project can deal with bacterial resistance problem on agriculture, and hope it can access on medicine one day.




Topic 1

 

 

<img src="2014placeholderimage.jpg" width="300px" height="300px">




Topic 2

<img src="2014placeholderimage.jpg" width="300px" height="300px">

<img src="2014placeholderimage.jpg" width="300px" height="300px">

 



Topic 3

 

<img src="2014placeholderimage.jpg" width="300px" height="200px">


</tr>

References

<img src="TCU-Line.png" width="100%">
^
   <a href="https://2014.igem.org/Team:TCU_Taiwan/Contact" title="Contact Us"><img src="TCU-Contact-bot.png" width="80" onMouseMove="this.src=Image0.src;" onMouseOut="this.src='https://static.igem.org/mediawiki/2014/8/84/TCU-Contact-bot.png'"></a>
   

<a href="mailto: tcutaiwan@gmail.com" target="_blank" title="Gmail"><img src="TCU%E3%84%A6Gmail.png" width="30"></a>  <a href="https://www.facebook.com/TCUiGEM?ref=ts&fref=ts" target="_blank" title="Facebook"><img src="TCU-Facebook.png" width="30"></a> <a href="https://www.youtube.com/channel/UCr40ZmaFNJGx7tUufCXK_OQ" target="_blank" title="YouTube"><img src="TCU_Youtube.png" width="30"></a> <a href="http://tcuigem.wordpress.com/" target="_blank" title="Wordpress"><img src="TCU-Wordpress.png" width="30"></a>
Team Members Project Parts Human Pratics Modeling Safety Notebook Attributions
<a href="https://2014.igem.org/Team:TCU_Taiwan/Team" title="Team Members"><img src="TCU-Team-logo.png" width="100"onMouseOver="this.width=this.width*1.1;Members.color='#333'" onMouseOut="this.width=this.width/1.1;Members.color='#F2DE79'"></a> <a href="https://2014.igem.org/Team:TCU_Taiwan/Project" title="Project"><img src="TCU-Project-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Project.color='#333'" onMouseOut="this.width=this.width/1.1;Project.color='#F2DE79'"></a> <a href="https://2014.igem.org/Team:TCU_Taiwan/Parts" title="Parts"><img src="TCU-Part-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Parts.color='#333'" onMouseOut="this.width=this.width/1.1;Parts.color='#F2DE79'"></a> <a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics" title="Human Pratics"><img src="TCU-Human-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Pratics.color='#333'" onMouseOut="this.width=this.width/1.1;Pratics.color='#F2DE79'"></a> <a href="https://2014.igem.org/Team:TCU_Taiwan/Modeling" title="Modeling"><img src="TCU-Modeling-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Modeling.color='#333'" onMouseOut="this.width=this.width/1.1;Modeling.color='#F2DE79'"></a> <a href="https://2014.igem.org/Team:TCU_Taiwan/Safety" title="Safety"><img src="TCU-Safety-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Safety.color='#333'" onMouseOut="this.width=this.width/1.1;Safety.color='#F2DE79'"></a> <a href="https://2014.igem.org/Team:TCU_Taiwan/Notebook" title="Notebook"><img src="TCU-Notebook-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Notebook.color='#333'" onMouseOut="this.width=this.width/1.1;Notebook.color='#F2DE79'"></a> <a href="https://2014.igem.org/Team:TCU_Taiwan/Attributions" title="Attributions"><img src="TCU-Attributions-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Attributions.color='#333'" onMouseOut="this.width=this.width/1.1;Attributions.color='#F2DE79'"></a>

Lost the way? Use it to help you if you're lost.

<a href="https://2014.igem.org/Team:TCU_Taiwan" title="Home"><img src="TCU-Logo-d.png" width="100"></a>

<img src="http://i.creativecommons.org/l/by/3.0/88x31.png" width="100">

<!==end of references -->

^
</html>