Team:UCL/experiments

From 2014.igem.org

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<a href="/Team:UCL/protocols"><span class="label label-warning">DNA extraction</span></a></div>
<a href="/Team:UCL/protocols"><span class="label label-warning">DNA extraction</span></a></div>
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<p>Our literature search identified a number of bacterial species that have been proven to degrade azo dye compounds including <i>B. subtilis</i> and <i>P. aeruginosa</i>.  We were able to obtain a <i>B. subtilis</i> strain for use in our project from ?.  We extracted the genomic DNA from this strain using a Promega Wizard Genomic DNA extraction kit so that we could subsequently amplify the azo-reducatase gene (AzoR1) and create our first azo-reductase BioBrick.  After completing the genomic DNA extracton we ran a gel to show that we had successfully extracted the <i>B. subtilis</i> genomic DNA.
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<p>Our literature search identified a number of bacterial species that have been proven to degrade azo dye compounds including <i>B. subtilis</i> and <i>P. aeruginosa</i>.  We were able to obtain a <i>B. subtilis</i> strain for use in our project from ?.  We extracted the genomic DNA from this strain using a Promega Wizard Genomic DNA extraction kit so that we could subsequently amplify the azo-reducatase gene (AzoR1) and create our first azo-reductase BioBrick.  After completing the genomic DNA extracton we ran a gel to show that we had successfully extracted the <i>B. subtilis</i> genomic DNA.</p>
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<h4>Transforming <i>E. coli</i> with Azo-reductase plasmids</h4>
<h4>Transforming <i>E. coli</i> with Azo-reductase plasmids</h4>

Revision as of 16:57, 6 September 2014

Goodbye Azo Dye : iGEM 2014 - University College London

 

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