Team:UCL/experiments
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<div class="byline"><i class="icon-user"></i> Adam Denyer <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013, 8:21 PM">October 15, 2013</abbr> | <div class="byline"><i class="icon-user"></i> Adam Denyer <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013, 8:21 PM">October 15, 2013</abbr> | ||
<strong> Protocols </strong> | <strong> Protocols </strong> | ||
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<a href="/Team:UCL/protocols"><span class="label label-warning">PCR</span></a> | <a href="/Team:UCL/protocols"><span class="label label-warning">PCR</span></a> | ||
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a> | <a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a> | ||
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<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a></div> | <a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a></div> | ||
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- | <p> | + | <p>We were gratefully provided with a number of plasmids containing azo-reductase genes from a group of researchers at the University of Lisbon, Portugal. As the DNA concentration of the plasmids we were sent was insufficient to perform PCR amplification on we transformed each of these plasmids into our <i>E. coli</i> NEB5lpha competent cells. After growing the cells overnight we then mini-prepped each of them to obtain plasmids at sufficient concentrations for future experimental work.</p> |
<h4>Experiment 3: Diagnostic digest of azo-reductase plasmids</h4> | <h4>Experiment 3: Diagnostic digest of azo-reductase plasmids</h4> |
Revision as of 15:40, 6 September 2014