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- | __NOTOC__
| + | #REDIRECT [[Team:Aachen/Notebook/Wetlab]] |
- | {{CSS/Main}}
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- | {{Team:Aachen/Stylesheet}}
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- | {{Team:Aachen/Header}}
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- | <span id="partners"></span>
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- | | + | |
- | =Our work in the lab=
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- | <html><ul class="team-grid">
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- | <!-- Overview -->
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- | | + | |
- | <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/April" style="color:black">
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- | <div class="team-item team-info" >
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- | <br/><br/>
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- | Summary of April
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- | <br/><br/>
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- | <!-- click for more information -->
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- | </div>
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- | <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/2/25/Aachen_14-10-10_April_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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- | </li>
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- | <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/May" style="color:black">
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- | <div class="team-item team-info" >
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- | <br/><br/>
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- | <b> May </b>
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- | <br/><br/>
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- | First work on BioBricks
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- | <br/><br/>
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- | <!-- click for more information -->
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- | </div>
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- | <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/ca/Aachen_team_member_Florian_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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- | </li>
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- | <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/June" style="color:black">
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- | <div class="team-item team-info" >
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- | <br/><br/>
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- | <b> June </b>
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- | <br/><br/>
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- | First creation of own BioBricks
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- | <br/><br/>
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- | <!-- click for more information -->
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- | </div>
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- | <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/88/Aachen_team_member_Arne_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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- | </li>
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- | | + | |
- | <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/July" style="color:black">
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- | <div class="team-item team-info" >
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- | <br/><br/>
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- | <b> July </b>
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- | <br/><br/>
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- | Improving our chip technology
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- | <br/><br/>
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- | <!-- click for more information -->
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- | </div>
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- | <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/48/Aachen_team_member_Nina_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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- | </li>
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- | | + | |
- | <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/August" style="color:black">
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- | <div class="team-item team-info" >
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- | <br/><br/>
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- | <b> August </b>
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- | <br/><br/>
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- | Adding Galectin-3 to our repertoire
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- | <br/><br/>
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- | <!-- click for more information -->
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- | </div>
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- | <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_team_member_Vera_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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- | </li>
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- | <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/September" style="color:black">
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- | <div class="team-item team-info" >
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- | <br/><br/>
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- | <b> September </b>
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- | <br/><br/>
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- | Getting into hot phase
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- | <br/><br/>
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- | <!-- click for more information -->
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- | </div>
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- | <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/7/77/Aachen_team_member_Philipp_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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- | </li>
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- | | + | |
- | <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/October" style="color:black">
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- | <div class="team-item team-info" >
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- | <br/><br/>
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- | <b> October </b>
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- | <br/><br/>
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- | Finalizing our project
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- | <br/><br/>
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- | <!-- click for more information -->
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- | </div>
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- | <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_team_member_Rene_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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- | </li>
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- | </ul></html>
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- | {{Team:Aachen/BlockSeparator}}
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- | | + | |
- | = April =
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- | == 1st ==
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- | '''Organization'''
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- | where to get:
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- | * key cards and keys for the lab
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- | * cooled centifuge
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- | * space in -80 °C
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- | * bottles for 70 % ethanol
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- | * ... (problems of a first year team ^^)
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- | | + | |
- | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/April Read more about April here...] | + | |
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- | {{Team:Aachen/BlockSeparator}}
| + | |
- | | + | |
- | = May =
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- | == 1st ==
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- | * gel with M - full -full - REACh1 SOE3.2 - REACH2 SOE3.2 - M
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- | *: → 120 V, 30 min
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- | *: → cut out the bands
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- | | + | |
- | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/May Read more about May here...] | + | |
- | | + | |
- | {{Team:Aachen/BlockSeparator}}
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- | | + | |
- | = June =
| + | |
- | == 3rd ==
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- | * transformation of 34 BioBricks
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- | | + | |
- | == 4th ==
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- | | + | |
- | * preparation of consumables:
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- | ** fresh 50 % glycerol
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- | ** new LB plates with cam and with kanamycin (kan)
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- | ** 60 glas tubes
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- | ** 2 L LB<sup>-</sup>
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- | ** 100 mL steril glas beads for plating
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- | | + | |
- | * master plates (6 clones per BioBrick) were made
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- | | + | |
- | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/June Read more about June here...]
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- | | + | |
- | {{Team:Aachen/BlockSeparator}}
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- | | + | |
- | = July =
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- | == 1st ==
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- | * transformation efficiency kit is broken
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- | → run an agarose gel with the kit plasmids to check for nuclease activity/ bands
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- | * inventory of the -80°C freezer was done
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- | | + | |
- | == 2nd ==
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- | * overnight cultures (LB) of K731520 and K1319042 for chips were inoculated
| + | |
- | | + | |
- | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/July Read more about July here...]
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- | | + | |
- | {{Team:Aachen/BlockSeparator}}
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- | | + | |
- | = August =
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- | == 1st ==
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- | * made electrocompetent ''E.coli'' rosetta cells.
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- | * prepared cultures of K1319042 and K131026
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- | | + | |
- | == 2nd ==
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- | * tested the OD measurement device and compared it to the spectrophotometer and the plate reader.
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- | * tested K131026 and K1319042 for fluorescence in the plate reader
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- | * did a heat shock transformation of I746909 into NEB TOP 10 cells
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- | * did an electroshock transformation of pET17-Gal3 into ''E.coli'' rosetta
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- | | + | |
- | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/August Read more about August here...]
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- | | + | |
- | {{Team:Aachen/BlockSeparator}}
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- | | + | |
- | = September =
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- | == 1st ==
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- | * 5 ml cultures of K1319003 and K1319004
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- | * plasmid prep
| + | |
- | {| class="wikitable"
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- | |-
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- | ! Plasmid !! DNA [ng/µl]
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- | |-
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- | | J23101.K516032 pSB1K3|| 23.5
| + | |
- | |-
| + | |
- | | J23115.K516032 pSB1K3|| 20.5
| + | |
- | |-
| + | |
- | | J04450 pSB1A3|| 57.5
| + | |
- | |-
| + | |
- | | J04450 pSB1K1|| 63.5
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- | |}
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- | * over night cultures of K131026 in DH5α and NEB
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- | | + | |
- | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/September Read more about September here...]
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- | {{Team:Aachen/BlockSeparator}}
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- | | + | |
- | = October =
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- | == 1st ==
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- | * Preparations for sensor-chip production on the following day (2014-10-02) was done accoringly to the [https://2014.igem.org/Team:Aachen/Notebook/Protocols#Agar_Chips| sensor chip manufacturing] protocol:
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- | ** At 18:30 we prepared over-night cultures from K1319042, B0015 and K131026 by inoculating 250 ml Erlenmeyer flasks each containing 50 ml LB medium . The flasks were incubated for ~12 hours at 37 °C on a shaker.
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- | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/October Read more about October here...]
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- | {{Team:Aachen/Footer}}
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