Team:Groningen/Template/MODULE/project/bandage/visionproblem

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Methods
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The problem, and our vision
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test 1
 
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<b>The problem - customer needs</b>
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test 2
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* Hospitals need to replace bandages several times a day, our bandage could inprove this to 3-4 days.
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* 3rd world countries need cheap and easy antibiotics - wound treatments,without increasing the risk at antibiotic resistance.
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* Burn wound centers are in enthousiastic about our bandage, because infections could be detected sooner.
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* A novel way is needed to fight antibiotic resistant strains<sup>4</sup>.
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<b>What would the ideal product be?</b>
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test 3
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*A product usable for hospitals and customers. Able to activate in 30 minutes and easy to neutralize.
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The problem - customer needs
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<b>What would it look like?</b>
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*A completely transparent bandage, this way the consumer of nurse can decide whether the wound needs attention or not.
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<div class="item">*. What would the ideal product be <div class="item">-A product useable for hospitals and customers. To be able to activate in 30 minutes and easy to neutralize</div></div>
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<div class="item">*. NWZ (MRSA obtained at the UMCG hospital)</div>
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<div class="item">*. CECT240 (<i>Staphylococcus aureus</i> subsp. <i>aureus</i>, Rosenbach 1884)</div>
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Used strains B. subtilus:
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<div class="item">1. ATCC6633 LB</div>
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<div class="item">2. ATCC6633 SMM (Preparation of SMM (Spizizen, 1958) with trace elements (Harwood and Archibald, 1990)</div>
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<div class="item">3. 168 LB</div>
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<b>What would it do?</b>
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01-10-2014
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*Cool down the wound, increase wound healing and avoid infection by <i>P. aeruginosa</i> and <i>S. aureus</i> and if infection already happened, the bandage will fight the infection.
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- Of all the S. aureus strains two different blood agar plates were made. The first set was named CAL1, NWZ1, CECT1, which meant that per plate 200 μl of sheep blood was added to 10 ml Colorado Blood agar. These were poured in different plates and afterwards 120 μl of each S. aureus strain was streaked out on top of it.
 
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- The second set was named CAL2, NWZ2, CECT2. In these plates 200 μl of sheep blood was added to 10 ml Colorado Blood agar per plate. These were poured on top of 120 μl of each S. aureus strain on each plate separately. This was mixed thoroughly so that the agar was mixed with the strain.
 
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- All the plates were dried under the fumehold.
 
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- On these six plates 6 μl of the three different sets of <i>B. subtilis</i> were spotted and put at 37 ºC for 24h.
 
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<b>Who is your external costumer?</b>
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02-10-2014
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* For now we aim on hospital usage. In this way the disposal and usage of the bandage can be controlled. We would like to bring it to the public as well but then we need to meet a lot more requirements.
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- All the diameters of the halo's were measured and the plates were discarded. (See results below).
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Latest revision as of 03:13, 18 October 2014

The problem, and our vision
 
The problem - customer needs
 

  • Hospitals need to replace bandages several times a day, our bandage could inprove this to 3-4 days.
  • 3rd world countries need cheap and easy antibiotics - wound treatments,without increasing the risk at antibiotic resistance.
  • Burn wound centers are in enthousiastic about our bandage, because infections could be detected sooner.
  • A novel way is needed to fight antibiotic resistant strains4.

What would the ideal product be?
 

  • A product usable for hospitals and customers. Able to activate in 30 minutes and easy to neutralize.

What would it look like?
 

  • A completely transparent bandage, this way the consumer of nurse can decide whether the wound needs attention or not.

What would it do?
 

  • Cool down the wound, increase wound healing and avoid infection by P. aeruginosa and S. aureus and if infection already happened, the bandage will fight the infection.

Who is your external costumer?
 

  • For now we aim on hospital usage. In this way the disposal and usage of the bandage can be controlled. We would like to bring it to the public as well but then we need to meet a lot more requirements.

Diffusion
 
 
Diffusion
 
Fighting the bacteria works! A great halo is shown.