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Team:Groningen/Template/MODULE/Notebook/toolbox/week10
From 2014.igem.org
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For each transformation, two seperate cultures were grown in LB with 10 μg/ml | For each transformation, two seperate cultures were grown in LB with 10 μg/ml | ||
chloramphenicol. A miniprep was done on the cultures and the insert size | chloramphenicol. A miniprep was done on the cultures and the insert size | ||
| - | was checked with colony PCR | + | was checked with colony PCR, using <a href="http://parts.igem.org/Part:BBa_G00100">VF2</a> and <a href="http://parts.igem.org/Part:BBa_G00101">VR</a> as |
| - | primers | + | primers. In addition, a restriction analysis was done using EcoRI and PstI. |
Cultures that showed a correct insert size were sent for sequencing. | Cultures that showed a correct insert size were sent for sequencing. | ||
</div> | </div> | ||
Latest revision as of 01:21, 18 October 2014
14 September - 21 September
Because of the constant low concentrations of the plasmid pSB1C3 with the
new BioBricks, it was decided to transform the isolated pSB1C3 with the
new BioBricks (NisA, NisC, NisRK, PNisI and sfGFP(Bs)) again in
Escherichia coli DH5α.
The pSB1C3 plasmids containing the new BioBricks were again isolated.
For each transformation, two seperate cultures were grown in LB with 10 μg/ml
chloramphenicol. A miniprep was done on the cultures and the insert size
was checked with colony PCR, using VF2 and VR as
primers. In addition, a restriction analysis was done using EcoRI and PstI.
Cultures that showed a correct insert size were sent for sequencing.
