Team:Groningen/Template/MODULE/Notebook/toolbox/week8

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Colony PCR on more transformants containing pSB1C3 with NisB and NisRK
Colony PCR on more transformants containing pSB1C3 with NisB and NisRK
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was repeated, using VF2 (BBa_G00100) and VR (BBa_G00101). This time, a  
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was repeated, using <a href="http://parts.igem.org/Part:BBa_G00100">VF2</a> and
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<a href="http://parts.igem.org/Part:BBa_G00101">VR</a>. This time, a  
correct insert size wasbfound for a NisRK transformant.<br>
correct insert size wasbfound for a NisRK transformant.<br>
Because there was still no correct insert size found for a NisB  
Because there was still no correct insert size found for a NisB  
transformant, the remaining NisB colonies were tested for a correct insert size.
transformant, the remaining NisB colonies were tested for a correct insert size.
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No positive transformants were found.
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There were no positive transformants found.
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Latest revision as of 01:21, 18 October 2014

1 September - 7 September
 
Colony PCR on more transformants containing pSB1C3 with NisB and NisRK was repeated, using VF2 and VR. This time, a correct insert size wasbfound for a NisRK transformant.
Because there was still no correct insert size found for a NisB transformant, the remaining NisB colonies were tested for a correct insert size. There were no positive transformants found.