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-	<h1>Mutation</h1>	+	<h1>Mutation·LABNOTE</h1>
		+	<p><b>May:</b> Amplify biobricks. </p>
		+	<p><b>June:</b> Clone the WT DNAQ ,recA,umuC,umuD from <em>E.coli</em>. </p>
		+	<p><b>July:</b> Perform the point-mutation and truncation of the four genes.</p>
		+	<p><b>August:</b> Add prefix and suffix to the genes;</p>
		+	<p>&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbspAdd RBS to the genes.</p>
		+	<p><b>September:</b> Construct the polycistron plasmid </p>
		+	<p>&nbsp&nbsp&nbsp&nbsp(DNAQ926+umuD’;umuC+recA730;umuD’+umuC+recA730;DNAQ926+umuD’+umuC+recA730;);
		+	<p>&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbspAdd promoter to construct the expression vector;
		+	<p>&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbspTest the mutation rate.</p>
		+	<p><b>October:</b> adapt the testing method and test the mutation rates.</p>
		+	<p>&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbspPrepare the biobrick for submission. </p>
		+
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Latest revision as of 22:30, 17 October 2014

Mutation·LABNOTE

May: Amplify biobricks.

June: Clone the WT DNAQ ,recA,umuC,umuD from E.coli.

July: Perform the point-mutation and truncation of the four genes.

August: Add prefix and suffix to the genes;

&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbspAdd RBS to the genes.

September: Construct the polycistron plasmid

&nbsp&nbsp&nbsp&nbsp(DNAQ926+umuD’;umuC+recA730;umuD’+umuC+recA730;DNAQ926+umuD’+umuC+recA730;); <p>&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbspAdd promoter to construct the expression vector; <p>&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbspTest the mutation rate.

October: adapt the testing method and test the mutation rates.

&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbspPrepare the biobrick for submission.