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Team:Groningen/Template/MODULE/Notebook/toolbox/week9
From 2014.igem.org
(Difference between revisions)
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The PCR was split into two seperate reactions because of the large | The PCR was split into two seperate reactions because of the large | ||
differences between the size of the different fragments. Fragments | differences between the size of the different fragments. Fragments | ||
| - | were sorted on <750 kb and >750 kb. | + | were sorted on <750 kb and >750 kb. The experiment was not continued |
because of time limits. | because of time limits. | ||
</div> | </div> | ||
Revision as of 20:27, 17 October 2014
8 - 14 September
A PCR was done on all the genes that contained illegal restriction sites:
NisI, NisT, NisP and NisFEG. This should generate multiple PCR fragments
for each gene, that will be assembled again using Gibson assembly.
The PCR was split into two seperate reactions because of the large
differences between the size of the different fragments. Fragments
were sorted on <750 kb and >750 kb. The experiment was not continued
because of time limits.
