Team:Utah State/BioBricks
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<h4>BBa_K1418012</h4> | <h4>BBa_K1418012</h4> | ||
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+ | <h4>Intermediate parts</h4> | ||
+ | </p> | ||
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+ | <p> | ||
+ | K208010 – Composite part of R0010 (a lac inducible promoter) and B0034 (a ribosome binding site with 1.0 level of efficiency) | ||
+ | </p> | ||
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+ | <p> | ||
+ | K1418011 – BioBrick twin of K118023 with double stop codons removed and designed as RFC 23 compatible. | ||
+ | </p> | ||
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+ | <p> | ||
+ | K844000 – A 10x-Histidine tag used to purify chlorophyllase enzymes using nickel affinity chromatography. RFC 23 compatible. | ||
+ | </p> | ||
+ | |||
+ | <p> | ||
+ | B0015 – Composite part of two transcriptional terminators. | ||
</p> | </p> | ||
Revision as of 20:10, 17 October 2014
BioBricks
Favorite Parts
BBa_K1418002
This part was constructed as a inducible chlorophyllase enzyme generator with a C-terminal 10x-Histidine tag to allow for simple purification using immobilized nickel affinity chromatography. Refer to our project page for background information on chlorophyllase and to our chlorophyllase results page to see how we produced, purified, and tested this enzyme.
Intermediate parts
K208010 – Composite part of R0010 (a lac inducible promoter) and B0034 (a ribosome binding site with 1.0 level of efficiency)
K1418001 – BioBrick encoding a chlorophyllase enzyme (TaCHL) found in Triticum aestivum, a common species of wheat. RFC 23 compatible.
K844000 – A 10x-Histidine tag used to purify chlorophyllase enzymes using nickel affinity chromatography. RFC 23 compatible.
B0015 – Composite part of two transcriptional terminators.
BBa_K1418012
Intermediate parts
K208010 – Composite part of R0010 (a lac inducible promoter) and B0034 (a ribosome binding site with 1.0 level of efficiency)
K1418011 – BioBrick twin of K118023 with double stop codons removed and designed as RFC 23 compatible.
K844000 – A 10x-Histidine tag used to purify chlorophyllase enzymes using nickel affinity chromatography. RFC 23 compatible.
B0015 – Composite part of two transcriptional terminators.
BBa_K1418061
<groupparts>iGEM014 Utah_State</groupparts>