"
Team:Groningen/Template/MODULE/project/bandage/visionproblem
From 2014.igem.org
(Difference between revisions)
(Created page with "<html> <!--Content module--> <div class="module contentmodule gridcell"> <div class="content"> <div class="wrapper"> <!-- TITLE SNIPPET START--> <div class="title"> Methods ...") |
|||
| Line 61: | Line 61: | ||
<div class="listing"> | <div class="listing"> | ||
| - | <div class="item">*. What would the ideal product be <div class="item">-A product useable for hospitals and customers. To be able to activate in 30 minutes and easy to neutralize</div></div> | + | <div class="item">*. What would the ideal product be <div class="item"> -A product useable for hospitals and customers. To be able to activate in 30 minutes and easy to neutralize</div></div> |
<div class="item">*. NWZ (MRSA obtained at the UMCG hospital)</div> | <div class="item">*. NWZ (MRSA obtained at the UMCG hospital)</div> | ||
<div class="item">*. CECT240 (<i>Staphylococcus aureus</i> subsp. <i>aureus</i>, Rosenbach 1884)</div> | <div class="item">*. CECT240 (<i>Staphylococcus aureus</i> subsp. <i>aureus</i>, Rosenbach 1884)</div> | ||
Revision as of 18:42, 17 October 2014
Methods
test 1
test 2
test 3
The problem - customer needs
*. What would the ideal product be
-A product useable for hospitals and customers. To be able to activate in 30 minutes and easy to neutralize
*. NWZ (MRSA obtained at the UMCG hospital)
*. CECT240 (Staphylococcus aureus subsp. aureus, Rosenbach 1884)
Used strains B. subtilus:
1. ATCC6633 LB
2. ATCC6633 SMM (Preparation of SMM (Spizizen, 1958) with trace elements (Harwood and Archibald, 1990)
3. 168 LB
01-10-2014
- Of all the S. aureus strains two different blood agar plates were made. The first set was named CAL1, NWZ1, CECT1, which meant that per plate 200 μl of sheep blood was added to 10 ml Colorado Blood agar. These were poured in different plates and afterwards 120 μl of each S. aureus strain was streaked out on top of it.
- The second set was named CAL2, NWZ2, CECT2. In these plates 200 μl of sheep blood was added to 10 ml Colorado Blood agar per plate. These were poured on top of 120 μl of each S. aureus strain on each plate separately. This was mixed thoroughly so that the agar was mixed with the strain.
- All the plates were dried under the fumehold.
- On these six plates 6 μl of the three different sets of B. subtilis were spotted and put at 37 ºC for 24h.
02-10-2014
- All the diameters of the halo's were measured and the plates were discarded. (See results below).
