Team:Evry/Notebook/Transposons/08.06.14
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<br/>It parallel annotation of plasmid pNK2 with Blast is realized. | <br/>It parallel annotation of plasmid pNK2 with Blast is realized. | ||
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Revision as of 16:30, 17 October 2014
It’s the first day or Brian Jester give us the plasmid pNK2. This plasmid contains transposon.
A Precultur of DH5α pir is realized.In a 15 mL tube, add 2 mL of LB medium and inoculate cells from glycerol. These cells can replicate the plasmid because they contains pi protein.
It parallel annotation of plasmid pNK2 with Blast is realized.