Team:Yale/Parts

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Yale/Parts&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:Yale"style="color:#000000">Home </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Yale"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:Yale/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:Yale/Parts"style="color:#000000"> Parts</a></td>
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<h1 style="margin-top:22px; font-size:50px;">Submitted Parts</h1>
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Our collection of submitted biobricks consists of:
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<ul style="list-style-type:square"> <li>Mussel foot protein (MFP) 1-5-1 sequence [combination of <i>Mytilus galloprovincialis</i> Foot Protein 5 (Mgfp-5) and <i>Mytilus Edulis</i> Foot Protein 1 (Mefp-1)]. <li>MFP with superfolder Green Fluorescence Protein (sfGFP).<li>MFP with our anti-microbial peptide, LL-37.<li> Entire construct of our anti-microbial adhesive peptide: 2XStrep_Flagtag--LL-37--Mussel Foot Protein--sfGFP. </ul>
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<i>Note all biobricks are in the pSB1C3 plasmid.</i>
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<td > <h3>What information do I need to start putting my parts on the Registry? </h3></td>
 
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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will submit them to the <a href="http://partsregistry.org"> Registry of Standard Biological Parts</a>. The iGEM software provides an easy way to present the parts your team has created. The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox. 
 
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<strong>Note that if you want to document a part you need to document it on the <a href="http://partsregistry.org Registry"> Registry</a>, not on your team wiki.</strong> Future teams and other users and are much more likely to find parts on the Registry than on your team wiki.
 
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<tr><td colspan="2"><h2>Full Construct: <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1396000">BBa_K1396000</a></a></h2>
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Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without a need to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
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The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.</p>
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<h3>When should you put parts into the Registry?</h3>
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<tr><td colspan="2"><h2>LL-37-MFP: <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1396001">BBa_K1396001</a></h2>
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As soon as possible! We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better recall you will have of all details surrounding your parts. Remember you don't need to send us the DNA to create an entry for a part on the Registry. However, you must send us the sample/DNA before the Jamboree. Only parts for which you have sent us samples/DNA are eligible for awards and medal requirements.
 
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The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine suppressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence suppressing LL-37 antimicrobial action.
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This is an improvement on the Utah State biobrick <a href= "http://parts.igem.org/Part:BBa_K1162006">BBa_K1162006 </a> which consists of only the LL-37 peptide.</p>
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The information needed to initially create a part on the Registry is:
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<tr><td colspan="2"><h2>MFP-sfGFP: <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1396002">BBa_K1396002</a></h2>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. Check out part <a href="http://parts.igem.org/Part:BBa_K404003">BBa_K404003</a> for an excellent example of a highly characterized part.  
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The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and superfolder GFP will allow for florescence imaging and localization. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.</p>
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You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry"> Add a Part to the Registry</a> link.
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<tr><td colspan="2"><h2>Mussel Foot Protein 1-5-1: <a href= "https://static.igem.org/mediawiki/2014/0/04/FP151.jpg">BBa_K1396003</a></h2>
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Recoded and codon optimized coding sequence for the mussel foot protein 151. TAG is recoded. In order to produce the protein co-express in cells contain either an L-DOPA orthogonal translation system or a Tyrosine suppressor.</p>
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<tr><td colspan="3" > <h3> Parts Table</h3></td></tr>
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Any parts your team has created will appear in this table below:</td></tr>
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<groupparts>iGEM013 Yale</groupparts>
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Latest revision as of 03:39, 17 October 2014

Submitted Parts

Our collection of submitted biobricks consists of:

  • Mussel foot protein (MFP) 1-5-1 sequence [combination of Mytilus galloprovincialis Foot Protein 5 (Mgfp-5) and Mytilus Edulis Foot Protein 1 (Mefp-1)].
  • MFP with superfolder Green Fluorescence Protein (sfGFP).
  • MFP with our anti-microbial peptide, LL-37.
  • Entire construct of our anti-microbial adhesive peptide: 2XStrep_Flagtag--LL-37--Mussel Foot Protein--sfGFP.

Note all biobricks are in the pSB1C3 plasmid.

Full Construct: BBa_K1396000

The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.

LL-37-MFP: BBa_K1396001

The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine suppressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence suppressing LL-37 antimicrobial action. This is an improvement on the Utah State biobrick BBa_K1162006 which consists of only the LL-37 peptide.

MFP-sfGFP: BBa_K1396002

The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and superfolder GFP will allow for florescence imaging and localization. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.

Mussel Foot Protein 1-5-1: BBa_K1396003

Recoded and codon optimized coding sequence for the mussel foot protein 151. TAG is recoded. In order to produce the protein co-express in cells contain either an L-DOPA orthogonal translation system or a Tyrosine suppressor.
Main Campus:
Molecular, Cellular & Developmental Biology
219 Prospect Street
P.O. Box 208103
New Haven, CT 06520
Phone: 203.432.3783
igem@yale.edu
natalie.ma@yale.edu (Graduate Advisor)
Copyright (c) 2014 Yale IGEM

Retrieved from "http://2014.igem.org/Team:Yale/Parts"