Team:Yale/Parts

From 2014.igem.org

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The collection consists of the BioBrick mussel foot protein (MFP) 1-5-1 sequence (combination of Mytilus galloprovincialis Foot Protein 5 (Mgfp-5) and Mytilus Edulis Foot Protein1 (Mefp-1)). The second BioBrick is the MFP with superfolder Green Fluorescence Protein (sfGFP). The third BioBrick is the MFP with our antimicrobial peptide of interest, LL-37. Finally, the fourth BioBrick is our entire construct of our antimicrobial peptide: 2XStrep_Flagtag_LL-37_Mussel Foot Protein_sfGFP. All biobricks are in the pSB1C3 plasmid.
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The collection consists of:
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The BioBrick mussel foot protein (MFP) 1-5-1 sequence (combination of Mytilus galloprovincialis Foot Protein 5 (Mgfp-5) and Mytilus Edulis Foot Protein1 (Mefp-1)). The second BioBrick is the MFP with superfolder Green Fluorescence Protein (sfGFP). The third BioBrick is the MFP with our antimicrobial peptide of interest, LL-37. Finally, the fourth BioBrick is our entire construct of our antimicrobial peptide: 2XStrep_Flagtag_LL-37_Mussel Foot Protein_sfGFP.  
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Note all biobricks are in the pSB1C3 plasmid.
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Revision as of 03:33, 17 October 2014

Submitted Parts

The collection consists of: The BioBrick mussel foot protein (MFP) 1-5-1 sequence (combination of Mytilus galloprovincialis Foot Protein 5 (Mgfp-5) and Mytilus Edulis Foot Protein1 (Mefp-1)). The second BioBrick is the MFP with superfolder Green Fluorescence Protein (sfGFP). The third BioBrick is the MFP with our antimicrobial peptide of interest, LL-37. Finally, the fourth BioBrick is our entire construct of our antimicrobial peptide: 2XStrep_Flagtag_LL-37_Mussel Foot Protein_sfGFP. Note all biobricks are in the pSB1C3 plasmid.

Full construct: BBa_K1396000

The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.

LL-37-MFP: BBa_K1396001

The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine suppressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence suppressing LL-37 antimicrobial action. This is an improvement on the Utah State biobrick BBa_K1162006 which consists of only the LL-37 peptide.

MFP-sfGFP: BBa_K1396002

The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and superfolder GFP will allow for florescence imaging and localization. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.

Mussel Foot Protein 1-5-1: BBa_K1396003

Recoded and codon optimized coding sequence for the mussel foot protein 151. TAG is recoded. In order to produce the protein co-express in cells contain either an L-DOPA orthogonal translation system or a Tyrosine suppressor.

Main Campus:
Molecular, Cellular & Developmental Biology
219 Prospect Street
P.O. Box 208103
New Haven, CT 06520
Phone: 203.432.3783
igem@yale.edu
natalie.ma@yale.edu (Graduate Advisor)
Copyright (c) 2014 Yale IGEM