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Team:Hannover/Protocols/Detection/Confocal
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<tr><td>Prepared MS Samples</td><td></td></tr> | <tr><td>Prepared MS Samples</td><td></td></tr> | ||
<tr><td>Confocal Microscope</td><td>(here:Eclipse TE2000-E, Nikon, Japan)</td></tr></table> | <tr><td>Confocal Microscope</td><td>(here:Eclipse TE2000-E, Nikon, Japan)</td></tr></table> | ||
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| - | + | <h2>Settings</h2><p class="text"><ol><li>100 x amplification<li>For excitation of fluorescence for confocal pictures using lasers with λ= 408 nm and λ= 488 nm <li>Transmission microscopy: halogen lamp<li>Detection of GFP signals: mercury lamp<li> | |
| - | <h2>Settings</h2><p class="text"><ol><li>< | + | </ol></p> |
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<p id="Fusszeile"><br><br><br><br><img src="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141010Fusszeile2.jpg" width="980px" style="float:right" /></p> | <p id="Fusszeile"><br><br><br><br><img src="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141010Fusszeile2.jpg" width="980px" style="float:right" /></p> | ||
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Revision as of 15:22, 15 October 2014
Protocols / How To: Confocal Microscopy
Material: |
|
| Prepared MS Samples | |
| Confocal Microscope | (here:Eclipse TE2000-E, Nikon, Japan) |
Settings
- 100 x amplification
- For excitation of fluorescence for confocal pictures using lasers with λ= 408 nm and λ= 488 nm
- Transmission microscopy: halogen lamp
- Detection of GFP signals: mercury lamp
