Team:Washington/Protocols

From 2014.igem.org

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<h1> Protocols </h1>
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<h2> Media, Plates and Solutions </h2>
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<h3> CCMB </h3>
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<h3> LB-Agar </h3>
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<h3> Luria Broth </h3>
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<h3> Super Optimal Broth (S.O.B.) </h3>
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<h2> Basic Cloning </h2>
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<h3> Polymerase Chain Reactions </h3>
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<h3> Restriction Endonuclease Reaction (Digestion) </h3>
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<h3> Ligation </h3>
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<h2> <i> Escherichia coli Protocols </i> </h2>
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<h3> Competent Cell Culturing </h3>
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<h3> Transformations </h3>
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<h3> Plating </h3>
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<h3> Overnights </h3>
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<h3> DNA-Extraction and mini-preps </h3>
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<h2> <i> Saccharomyces cerevisiae </i> </h2>
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<h3> Transformations </h3>
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<h2> Flow Cytometry and Fluorescence Activated Cell Sorting </h2>
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<h2> Circluar Dichroism, Thermal Melts and Guandinium Hydrogen Chloride Melts </h2>
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Revision as of 01:44, 9 October 2014

UW Homepage Official iGEM website

Contents

Protocols

Media, Plates and Solutions

CCMB

LB-Agar

Luria Broth

Super Optimal Broth (S.O.B.)

Basic Cloning

Polymerase Chain Reactions

Restriction Endonuclease Reaction (Digestion)

Ligation

Escherichia coli Protocols

Competent Cell Culturing

Transformations

Plating

Overnights

DNA-Extraction and mini-preps

Saccharomyces cerevisiae

Transformations

Flow Cytometry and Fluorescence Activated Cell Sorting

Circluar Dichroism, Thermal Melts and Guandinium Hydrogen Chloride Melts

</html>

Retrieved from "http://2014.igem.org/Team:Washington/Protocols"