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Team:Evry/Interlab Study/Goal
From 2014.igem.org
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<h2>Goal of the iGEM 2014 Measurement Interlab Study</h2> | <h2>Goal of the iGEM 2014 Measurement Interlab Study</h2> | ||
<br/>The goal of the interlab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around the world. | <br/>The goal of the interlab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around the world. | ||
| - | <br/>The study is also extended to the entire Anderson library of constitutive prokaryotic promoters (J23100-J23119). This library correspond to 19 promoters containing some nucleotide mutations on the -35 and the -10. The idea is to compare expression strength of promoters according to mutation, from the maximum amount of fluorescence data to have significative results. | + | <br/>The study is also extended to the entire Anderson library of constitutive prokaryotic promoters (J23100-J23119). This library correspond to 19 promoters containing some nucleotide mutations on the -35 and the -10. The idea is to compare expression strength of promoters according to mutation, from the maximum amount of fluorescence data to have significative results. |
| + | <br/> To test the expression of promoters, they are cloned in the PSB1C3 vector followed by the BBa_E0240, a GFP generator. Constructions are introduced in E. coli DH5 alpha Our team decided to test the fluorescence with a TECAN infiniteM200 in 96 well plates. | ||
| + | <br/> | ||
<div class="center"> | <div class="center"> | ||
Revision as of 17:10, 19 September 2014
Goal of the iGEM 2014 Measurement Interlab Study
The goal of the interlab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around the world.
The study is also extended to the entire Anderson library of constitutive prokaryotic promoters (J23100-J23119). This library correspond to 19 promoters containing some nucleotide mutations on the -35 and the -10. The idea is to compare expression strength of promoters according to mutation, from the maximum amount of fluorescence data to have significative results.
To test the expression of promoters, they are cloned in the PSB1C3 vector followed by the BBa_E0240, a GFP generator. Constructions are introduced in E. coli DH5 alpha Our team decided to test the fluorescence with a TECAN infiniteM200 in 96 well plates.
http://parts.igem.org/Promoters/Catalog/Anderson
Goal
