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Team:Evry/Notebook/Sensing/07-29-2014
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<img src="https://static.igem.org/mediawiki/2014/9/96/Pouet2.jpg" alt="pouet" /> <br> | <img src="https://static.igem.org/mediawiki/2014/9/96/Pouet2.jpg" alt="pouet" /> <br> | ||
| - | + | 1. Serial dilutions of compound were made <br> | |
| + | |||
| + | <li>Lead <br> | ||
<img src="https://static.igem.org/mediawiki/2014/6/6d/Dilutionlead.jpg" alt="text to print if image not found" /> <br> | <img src="https://static.igem.org/mediawiki/2014/6/6d/Dilutionlead.jpg" alt="text to print if image not found" /> <br> | ||
| + | <li>Cadmium <br> | ||
<img src="https://static.igem.org/mediawiki/2014/7/7e/Dilutioncadmium.jpg" alt="text to print if image not found" /> <br> | <img src="https://static.igem.org/mediawiki/2014/7/7e/Dilutioncadmium.jpg" alt="text to print if image not found" /> <br> | ||
| + | <li>Nitrite <br> | ||
<img src="https://static.igem.org/mediawiki/2014/3/39/Dilutionnitrite.jpg" alt="text to print if image not found" /> <br> | <img src="https://static.igem.org/mediawiki/2014/3/39/Dilutionnitrite.jpg" alt="text to print if image not found" /> <br> | ||
<br> | <br> | ||
| - | + | 2. 300µL of E.coli BL21 was added in each eppendorf tube.<br> | |
| - | We had 2 control tubes: | + | We had 2 control tubes: <br> |
| - | A negative control: 2,7mL of MB medium + 300µL of compound | + | *A negative control: 2,7mL of MB medium + 300µL of compound<br> |
| - | A positive control: 2,7mL of MB medium + 300µL of E.coli BL21 | + | *A positive control: 2,7mL of MB medium + 300µL of E.coli BL21 |
| - | + | <br> | |
| - | < | + | <br> |
| + | 3. Incubation of tubes at 30°C overnight, 200rpm | ||
</p> | </p> | ||
Latest revision as of 10:32, 8 September 2014
Survivability in molecules which are going to be sensed:
Ranges of concentrations of molecules that we want to sense were made as described in following tables:
1. Serial dilutions of compound were made
2. 300µL of E.coli BL21 was added in each eppendorf tube.
We had 2 control tubes:
*A negative control: 2,7mL of MB medium + 300µL of compound
*A positive control: 2,7mL of MB medium + 300µL of E.coli BL21
3. Incubation of tubes at 30°C overnight, 200rpm Jul 29
