"
Team:Evry/Notebook/Sensing/07-29-2014
From 2014.igem.org
(Difference between revisions)
| Line 21: | Line 21: | ||
2. 300µL of E.coli BL21 was added in each eppendorf tube.<br> | 2. 300µL of E.coli BL21 was added in each eppendorf tube.<br> | ||
| - | We had 2 control tubes: | + | We had 2 control tubes: <br> |
| - | A negative control: 2,7mL of MB medium + 300µL of compound | + | *A negative control: 2,7mL of MB medium + 300µL of compound<br> |
| - | A positive control: 2,7mL of MB medium + 300µL of E.coli BL21 | + | *A positive control: 2,7mL of MB medium + 300µL of E.coli BL21 |
<br> | <br> | ||
<br> | <br> | ||
Revision as of 09:57, 8 September 2014
Survivability in molecules which are going to be sensed:
Ranges of concentrations of molecules that we want to sense were made as described in following tables:
1. Serial dilutions of compound was made from 10(-2) mol/L to 10(-8) mol/L
2. 300µL of E.coli BL21 was added in each eppendorf tube.
We had 2 control tubes:
*A negative control: 2,7mL of MB medium + 300µL of compound
*A positive control: 2,7mL of MB medium + 300µL of E.coli BL21
3. Incubation of tubes at 30°C overnight, 200rpm Jul 29
