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Team:Evry/Notebook/Sensing/07-29-2014
From 2014.igem.org
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<u><b>Survivability in molecules which are going to be sensed: </u></b> <br> | <u><b>Survivability in molecules which are going to be sensed: </u></b> <br> | ||
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Ranges of concentrations of molecules that we want to sense were made as described in following tables:<br> | Ranges of concentrations of molecules that we want to sense were made as described in following tables:<br> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/9/96/Pouet2.jpg" alt="pouet" /> <br> | ||
| - | <img src="https://static.igem.org/mediawiki/2014/ | + | <li>Serial dilutions of compound was made from 10(-2) mol/L to 10(-8) mol/L <br> |
| + | <img src="https://static.igem.org/mediawiki/2014/6/6d/Dilutionlead.jpg" alt="text to print if image not found" /> <br> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/7/7e/Dilutioncadmium.jpg" alt="text to print if image not found" /> <br> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/3/39/Dilutionnitrite.jpg" alt="text to print if image not found" /> <br> | ||
<br> | <br> | ||
| - | + | <li>300µL of E.coli BL21 was added in each eppendorf tube. <br> | |
| + | We had 2 control tubes: | ||
| + | A negative control: 2,7mL of MB medium + 300µL of compound | ||
| + | A positive control: 2,7mL of MB medium + 300µL of E.coli BL21 | ||
| + | <li>Incubation of tubes at 30°C overnight, 200rpm | ||
</p> | </p> | ||
Revision as of 09:32, 8 September 2014
Survivability in molecules which are going to be sensed:
Ranges of concentrations of molecules that we want to sense were made as described in following tables:
We had 2 control tubes: A negative control: 2,7mL of MB medium + 300µL of compound A positive control: 2,7mL of MB medium + 300µL of E.coli BL21
