LOCUS Exported 4454 bp ds-DNA circular SYN 17-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0097_pluxRRR12y-sfGFP-stRBS-luxI SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 4454) AUTHORS Daniel Gerngross TITLE Direct Submission JOURNAL Exported 17 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4454 /organism="recombinant plasmid" /mol_type="other DNA" terminator 1..126 /note="T0" terminator 27..121 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" misc_feature 127..196 /note="R2oDNA spacer" /note="R2oDNA 70bp spacer 13" terminator 203..230 /note="T7Te" /note="phage T7 early transcription terminator" misc_feature complement(238..308) /note="taR12y" promoter complement(309..363) /note="lux rR (R0062)" misc_feature 364..433 /note="spacer (B0040)" promoter 434..488 /note="lux rR (R0062)" 3'UTR 489..540 /note="crR12y" RBS 523..534 /note="RBS (B0034)" CDS 541..1266 /codon_start=1 /note="sfGFP" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYKRP*" RBS 1275..1286 /note="RBS (B0034)" CDS 1293..1877 /codon_start=1 /note="luxI (C0161)" /translation="MTIMIKKSDFLAIPSEEYKGILSLRYQVFKQRLEWDLVVENNLES DEYDNSNAEYIYACDDTENVSGCWRLLPTTGDYMLKSVFPELLGQQSAPKDPNIVELSR FAVGKNSSKINNSASEITMKLFEAIYKHAVSQGITEYVTVTSTAIERFLKRIKVPCHRI GDKEIHVLGDTKSVVLSMPINEQFKKAVLN*" terminator 1889..1975 /gene="Escherichia coli rrnB" /note="T1" /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1969..1996 /note="T1" primer_bind 2003..2022 /note="AB179" primer_bind 2188..2207 /note="AB5" rep_origin 2341..3168 /direction=RIGHT /note="p15A" rep_origin 2589..2591 /direction=RIGHT /note="P15 ori from pACYC184" rep_origin complement(2592..3136) /direction=LEFT /note="p15A ori" /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." primer_bind 3240..3259 /note="AB180" oriT 3270..3378 /note="incP origin of transfer" misc_feature 3350 /note="C->T no real mutation, just for testing" CDS complement(3534..4349) /note="KamR" ORIGIN 1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg 61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 121 ccaagatatt cgtcacagta gttcaacctc agatacagaa accgtagatt gggattcagg 181 aagttaccct attgtttata aacgcagaaa ggcccacccg aaggtgagcc agtgtgatct 241 agagatatat ggtagtagta agttaatttt cattaaccac cactaccaat cacctcctgg 301 atttagcttt tattcgacta taacaaacca ttttcttgcg taaacctgta cgatcctaca 361 ggtaggttct gttaagtaac tgaacccaat gtcgttagtg acgcttacct cttaagaggt 421 cactgaccta acaacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt 481 cgaataaaga attctaccat tcacctcttg gatttagcta ttaaagagga gaaaggtacc 541 atgagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt 601 gatgttaatg ggcacaaatt ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga 661 aaactcaccc ttaaatttat ttgcactact ggaaaactac ctgttccgtg gccaacactt 721 gtcactactc tgacctatgg tgttcaatgc ttttcccgtt atccggatca catgaaacgg 781 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc 841 aaagatgacg ggacctacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt 901 aatcgtatcg agttaaaggg tattgatttt aaagaagatg gaaacattct tggacacaaa 961 ctcgagtaca actttaactc acacaatgta tacatcacgg cagacaaaca aaagaatgga 1021 atcaaagcta acttcaaaat tcgccacaac gttgaagatg gttccgttca actagcagac 1081 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac 1141 ctgtcgacac aatctgtcct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt 1201 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaaaggcct 1261 taataatact agtgaaagag gagaaatact agatgactat aatgataaaa aaatcggatt 1321 ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat caagtgttta 1381 agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat gagtatgata 1441 actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt ggatgctggc 1501 gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc 1561 aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct gtaggtaaaa 1621 atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt gaagctatat 1681 ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca acagcaatag 1741 agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa gaaattcatg 1801 tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag tttaaaaaag 1861 cagtcttaaa ttaataaatt aaaggcatca aataaaacga aaggctcagt cgaaagactg 1921 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc 1981 gccctagaca gctgggcgcg ccaaatacgc ccggtagtga tcttatttca ttatggtgaa 2041 agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct 2101 tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag 2161 gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga 2221 tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct 2281 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta 2341 tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag 2401 aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc 2461 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg 2521 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg 2581 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca 2641 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc cctggcggct 2701 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc 2761 cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg 2821 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt 2881 cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga 2941 tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt 3001 tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac 3061 cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag 3121 accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct agcatgagcg 3181 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 3241 gaaaagtgcc acctgctagg gccggcctac ggccagcctc gcagagcagg attcccgttg 3301 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgt gggtgggcct 3361 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc 3421 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata 3481 atgaccccga agcagggtta tgcagcggaa aaggaccctt gtccaattaa ttattagaaa 3541 aattcatcca gcatcagatg aaattgcagt ttgttcatat ccggattatc aatgccatat 3601 ttctgaaaca gacgtttttg caggctcggg ctaaattcgc ccaggcagtt ccacagaatg 3661 gccagatcct gataacgatc cgcaatgccc acacggccca catcaatgca gccaatcagt 3721 ttgccttcat cgaaaatcag gttatccagg ctaaaatcgc cgtgggtcac cacgctatcc 3781 gggctaaacg gcagcagttt atgcatttct ttccacacct gttccaccgg ccagccgtta 3841 cgttcatcat caaaatcgct cgcatccacc aggccgttgt tcatacggct ctgcgcctgg 3901 gccagacgaa acacacgatc gctgttaaac gggcagttgc acaccggaat gctatgcaga 3961 cgacgcagaa acacggccag cgcatccaca atgttttcgc cgctatccgg atattcttcc 4021 agcacctgaa acgcggtttt gcccggaatc gcggtggtca gcagccacgc atcatccggg 4081 gtgcgaataa aatgtttaat ggtcggcagc ggcataaatt cggtcagcca gttcagacgc 4141 accatttcat cggtcacatc gttcgccacg ctgcctttgc catgtttcag aaacagttcc 4201 ggcgcatccg gtttgccata cagacgataa atggtcgcgc cgctctgacc cacgttatca 4261 cgcgcccatt tatagccata cagatccgca tccatgttgc tgttcagacg cggacggcta 4321 cagctcgttt cacgctgaat atggctcata acaccccttg tattactgtt tatgtaagca 4381 gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt 4441 tgagacacaa attt //