LOCUS Exported 3904 bp ds-DNA circular SYN 10-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0040_regulator_lasR_corrected SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 3904) AUTHORS Daniel Gerngross TITLE Direct Submission JOURNAL Exported 10 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3904 /organism="recombinant plasmid" /mol_type="other DNA" terminator 12..98 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" misc_feature 280..471 /note="ROP" misc_feature 573..713 /note="bom" /note="basis of mobility region from pBR322" rep_origin complement(884..1503) /direction=LEFT /note="pBR322_origin" oriT 1523..1631 /note="incP origin of transfer" CDS complement(1821..2681) /note="AmpR" promoter complement(2682..2773) /gene="bla" /note="AmpR promoter" terminator 2846..2940 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" promoter 2984..3018 /note="const. promoter (J23100)" RBS 3027..3038 /note="RBS (B0034)" CDS 3045..3767 /codon_start=1 /note="lasR (C0179)" /translation="MALVDGFLELERSSGKLEWSAILQKMASDLGFSKILFGLLPKDSQ DYENAFIVGNYPAAWREHYDRAGYARVDPTVSHCTQSVLPIFWEPSIYQTRKQHEFFEE ASAAGLVYGLTMPLHGARGELGALSLSVEAENRAEANRFIESVLPTLWMLKDYALQSGA GLAFEHPVSKPVVLTSREKEVLQWCAIGKTSWEISVICNCSEANVNFHMGNIRRKFGVT SRRVAAIMAVNLGLITL*" terminator 3776..3904 /note="directional double term (B0015)" ORIGIN 1 attaaaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 61 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acagctgggc 121 gcgcccccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc 181 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac 241 ccccatgaac agaaattccc ccttacacgg aggcatcaag tgaccaaaca ggaaaaaacc 301 gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac 361 gagctggacg cggatgaaca ggcagacatc tgtgaatcgc tgcacgacca cgctgatgag 421 ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 481 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 541 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat 601 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc 661 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 721 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 781 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 841 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 901 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 961 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 1021 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 1081 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 1141 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 1201 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 1261 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 1321 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 1381 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 1441 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 1501 tcttttctac ggggccggcc tacggccagc ctcgcagagc aggattcccg ttgagcaccg 1561 ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac 1621 ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc 1681 aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc 1741 cgaagcaggg ttatgcagcg gaaaaggaca agggtcgtcc aaaaaaaaag gctccaaaag 1801 gagcctttaa ttgtatcggt ttaccaatgt ttaatcaggc tcgcgccaat ttccgcaatc 1861 tgacggttac gttcatccat cgtcgcctgg ctgccggtgg tataaatcac cacaatacgg 1921 ctcggtttac catccgggcc cagcgccgca ataatgccac ggctgccacg ttcgcccgca 1981 ccgcttttat ccgcaataaa ccagccagcc ggcagcgcgc tacgcagcag cggacccgcc 2041 actttatccg cttccatcca atcaatcagt tgctggcggc ttgccagggt cagcagctca 2101 cccgtcagca gtttacgcag ggtggtcgcc attgctgccg gcatggtggt atcacgttca 2161 tcgttcggaa tcgcttcgtt cagttccggt tcccaacgat ccagacgggt cacatgatcg 2221 cccatgttat gcagaaacgc ggtcagttct ttcggaccgc caatggtcgt cagcagcagg 2281 ttcgccgcgg tgttatcgct catggtaatc gccgcgctgc acagttcacg cacggtcatg 2341 ccatcggtca gatgtttttc ggtcaccggg ctatattcca ccagatcgtt ctggctataa 2401 tgaatacgac ggcccagttg ttcctggccc gcatccacac ggctcagaac cgcaccgcac 2461 agcagcactt taaaggtgct catcatcgga aaacgttctt ccggacgaaa agattccaga 2521 attttgccgc tgttcagatc cagttcaata tagcccacgc gcgcacccag ttgatcttcc 2581 gcatctttca ctttcaccag ggtttccgga tgcgcaaaca ccggcaggca aaacgccgca 2641 aaaaacggaa tcagcgccac acgaaaatgc tgaatgctca tactcttcct ttttcaatat 2701 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 2761 aaaaataaac aaatagggga attaaaaaaa agcccgctca ttaggcgggc tactaattta 2821 aatcgtaatt attggggacc cctggattct caccaataaa aaacgcccgg cggcaaccga 2881 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc 2941 caagactagt cgccagggtt ttcccagtca cgacgcggcc gcattgacgg ctagctcagt 3001 cctaggtaca gtgctagcta ctagagaaag aggagaaata ctagatggcc ttggttgacg 3061 gttttcttga gctggaacgc tcaagtggaa aattggagtg gagcgccatc ctccagaaga 3121 tggcgagcga ccttggattc tcgaagatcc tgttcggcct gttgcctaag gacagccagg 3181 actacgagaa cgccttcatc gtcggcaact acccggccgc ctggcgcgag cattacgacc 3241 gggctggcta cgcgcgggtc gacccgacgg tcagtcactg tacccagagc gtactgccga 3301 ttttctggga accgtccatc taccagacgc gaaagcagca cgagttcttc gaggaagcct 3361 cggccgccgg cctggtgtat gggctgacca tgccgctgca tggtgctcgc ggcgaactcg 3421 gcgcgctgag cctcagcgtg gaagcggaaa accgggccga ggccaaccgt ttcatagagt 3481 cggtcctgcc gaccctgtgg atgctcaagg actacgcact gcaaagcggt gccggactgg 3541 ccttcgaaca tccggtcagc aaaccggtgg ttctgaccag ccgggagaag gaagtgttgc 3601 agtggtgcgc catcggcaag accagttggg agatatcggt tatctgcaac tgctcggaag 3661 ccaatgtgaa cttccatatg ggaaatattc ggcggaagtt cggtgtgacc tcccgccgcg 3721 tagcggccat tatggccgtt aatttgggtc ttattactct ctaataatac tagagccagg 3781 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 3841 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 3901 tata //