LOCUS Exported 3843 bp ds-DNA circular SYN 17-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0110_prhlRRR12-sfGFP_EcoRI-_XbaI- SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 3843) AUTHORS Daniel Gerngross TITLE Direct Submission JOURNAL Exported 17 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3843 /organism="recombinant plasmid" /mol_type="other DNA" terminator 1..126 /note="T0" terminator 27..121 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" misc_feature 127..196 /note="R2oDNA spacer" /note="R2oDNA 70bp spacer 29" terminator 203..230 /note="T7Te" /note="phage T7 early transcription terminator" misc_feature complement(238..312) /note="taR12" promoter complement(313..363) /note="P(Rhl) (I14017)" misc_feature 364..433 /note="spacer (B0040)" promoter 434..484 /note="P(Rhl) (I14017)" misc_feature 485..540 /note="crR12" RBS 523..534 /note="RBS (B0034)" CDS 541..1266 /codon_start=1 /note="sfGFP" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYKRP*" terminator 1269..1385 /note="T1" terminator 1278..1364 /gene="Escherichia coli rrnB" /note="T1" /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 1392..1411 /note="AB179" primer_bind 1577..1596 /note="AB5" rep_origin 1730..2557 /direction=RIGHT /note="p15A" rep_origin 1978..1980 /direction=RIGHT /note="P15 ori from pACYC184" rep_origin complement(1981..2525) /direction=LEFT /note="p15A ori" /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." primer_bind 2629..2648 /note="AB180" oriT 2659..2767 /note="incP origin of transfer" misc_feature 2739 /note="C->T just for test, no real mutation" CDS complement(2923..3738) /note="KamR" ORIGIN 1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg 61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 121 ccaagatgtg agtcctgtta cgcccgtgtt ttccagtaga ataatagtga caatcgtttc 181 agataagcaa ccaatatata aacgcagaaa ggcccacccg aaggtgagcc agtgtgatct 241 agctagagat atatggtagt agtaagttaa ttttcattaa ccaccactac caatcacctc 301 ctggatttgg gtttcgacta taacaaacca ttttcttgcg taagcctacc aattcatgca 361 ggaaggttct gttaagtaac tgaacccaat gtcgttagtg acgcttacct cttaagaggt 421 cactgaccta acatcctgca tgaattggta ggcttacgca agaaaatggt ttgttatagt 481 cgaagaatta attctaccat tcacctcttg gatttgggta ttaaagagga gaaaggtacc 541 atgagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt 601 gatgttaatg ggcacaaatt ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga 661 aaactcaccc ttaaatttat ttgcactact ggaaaactac ctgttccgtg gccaacactt 721 gtcactactc tgacctatgg tgttcaatgc ttttcccgtt atccggatca catgaaacgg 781 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc 841 aaagatgacg ggacctacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt 901 aatcgtatcg agttaaaggg tattgatttt aaagaagatg gaaacattct tggacacaaa 961 ctcgagtaca actttaactc acacaatgta tacatcacgg cagacaaaca aaagaatgga 1021 atcaaagcta acttcaaaat tcgccacaac gttgaagatg gttccgttca actagcagac 1081 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac 1141 ctgtcgacac aatctgtcct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt 1201 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaaaggcct 1261 taataaatta aaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 1321 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ccctagacag 1381 ctgggcgcgc caaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct 1441 cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca 1501 acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg 1561 gcgcaaagtg cgtcgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg 1621 aggtgctcca gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt 1681 ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctag cggagtgtat actggcttac 1741 tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg 1801 caccggtgcg tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact 1861 cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt 1921 tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt 1981 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg 2041 gcgaaacccg acaggactat aaagatacca ggcgtttccc ctggcggctc cctcgtgcgc 2101 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct 2161 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca 2221 cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 2281 cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt 2341 tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg 2401 ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac 2461 cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat 2521 ctcaagaaga tcatcttatt aatcagataa aatatttcta gcatgagcgg atacatattt 2581 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 2641 cctgctaggg ccggcctacg gccagcctcg cagagcagga ttcccgttga gcaccgccag 2701 gtgcgaataa gggacagtga agaaggaaca cccgctcgtg ggtgggccta cttcacctat 2761 cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa 2821 tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa 2881 gcagggttat gcagcggaaa aggacccttg tccaattaat tattagaaaa attcatccag 2941 catcagatga aattgcagtt tgttcatatc cggattatca atgccatatt tctgaaacag 3001 acgtttttgc aggctcgggc taaattcgcc caggcagttc cacagaatgg ccagatcctg 3061 ataacgatcc gcaatgccca cacggcccac atcaatgcag ccaatcagtt tgccttcatc 3121 gaaaatcagg ttatccaggc taaaatcgcc gtgggtcacc acgctatccg ggctaaacgg 3181 cagcagttta tgcatttctt tccacacctg ttccaccggc cagccgttac gttcatcatc 3241 aaaatcgctc gcatccacca ggccgttgtt catacggctc tgcgcctggg ccagacgaaa 3301 cacacgatcg ctgttaaacg ggcagttgca caccggaatg ctatgcagac gacgcagaaa 3361 cacggccagc gcatccacaa tgttttcgcc gctatccgga tattcttcca gcacctgaaa 3421 cgcggttttg cccggaatcg cggtggtcag cagccacgca tcatccgggg tgcgaataaa 3481 atgtttaatg gtcggcagcg gcataaattc ggtcagccag ttcagacgca ccatttcatc 3541 ggtcacatcg ttcgccacgc tgcctttgcc atgtttcaga aacagttccg gcgcatccgg 3601 tttgccatac agacgataaa tggtcgcgcc gctctgaccc acgttatcac gcgcccattt 3661 atagccatac agatccgcat ccatgttgct gttcagacgc ggacggctac agctcgtttc 3721 acgctgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat 3781 tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaaa 3841 ttt //