LOCUS Exported 3910 bp ds-DNA circular SYN 10-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0042_regulator_rhlR_corrected SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 3910) AUTHORS Daniel Gerngross TITLE piG0042 JOURNAL Exported 10 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3910 /organism="recombinant plasmid" /mol_type="other DNA" misc_feature 259..450 /note="ROP" misc_feature 552..692 /note="bom" /note="basis of mobility region from pBR322" rep_origin complement(863..1482) /direction=LEFT /note="pBR322_origin" oriT 1502..1610 /note="incP origin of transfer" CDS complement(1800..2660) /note="AmpR" promoter complement(2661..2752) /gene="bla" /note="AmpR promoter" terminator 2825..2919 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" promoter 2963..2997 /note="const. promoter (J23100)" RBS 3006..3017 /note="RBS (B0034)" CDS 3024..3752 /codon_start=1 /product="RhlR (no LVA)" /note="rhlR (C0171)" /translation="MRNDGGFLLWWDGLRSEMQPIHDSQGVFAVLEKEVRRLGFDYYAY GVRHTIPFTRPKTEVHGTYPKAWLERYQMQNYGAVDPAILNGLRSSEMVVWSDSLFDQS RMLWNEARDWGLCVGATLPIRAPNNLLSVLSVARDQQNISSFEREEIRLRLRCMIELLT QKLTDLEHPMLMSNPVCLSHREREILQWTADGKSSGEIAIILSISESTVNFHHKNIQKK FDAPNKTLAAAYAAALGLI*" terminator 3761..3889 /note="directional double term (B0015)" terminator 3901..77 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 61 tcctgagtag gacaaatccg ccgccctaga cagctgggcg cgcccccgcc gcatccatac 121 cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg 181 tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaattcccc 241 cttacacgga ggcatcaagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt 301 atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag 361 gcagacatct gtgaatcgct gcacgaccac gctgatgagc tttaccgcag ctgcctcgcg 421 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 481 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 541 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta 601 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 661 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact 721 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 781 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 841 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 901 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 961 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 1021 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 1081 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 1141 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 1201 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 1261 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 1321 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 1381 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 1441 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggccggcct 1501 acggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag 1561 tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc 1621 gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga 1681 aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg 1741 aaaaggacaa gggtcgtcca aaaaaaaagg ctccaaaagg agcctttaat tgtatcggtt 1801 taccaatgtt taatcaggct cgcgccaatt tccgcaatct gacggttacg ttcatccatc 1861 gtcgcctggc tgccggtggt ataaatcacc acaatacggc tcggtttacc atccgggccc 1921 agcgccgcaa taatgccacg gctgccacgt tcgcccgcac cgcttttatc cgcaataaac 1981 cagccagccg gcagcgcgct acgcagcagc ggacccgcca ctttatccgc ttccatccaa 2041 tcaatcagtt gctggcggct tgccagggtc agcagctcac ccgtcagcag tttacgcagg 2101 gtggtcgcca ttgctgccgg catggtggta tcacgttcat cgttcggaat cgcttcgttc 2161 agttccggtt cccaacgatc cagacgggtc acatgatcgc ccatgttatg cagaaacgcg 2221 gtcagttctt tcggaccgcc aatggtcgtc agcagcaggt tcgccgcggt gttatcgctc 2281 atggtaatcg ccgcgctgca cagttcacgc acggtcatgc catcggtcag atgtttttcg 2341 gtcaccgggc tatattccac cagatcgttc tggctataat gaatacgacg gcccagttgt 2401 tcctggcccg catccacacg gctcagaacc gcaccgcaca gcagcacttt aaaggtgctc 2461 atcatcggaa aacgttcttc cggacgaaaa gattccagaa ttttgccgct gttcagatcc 2521 agttcaatat agcccacgcg cgcacccagt tgatcttccg catctttcac tttcaccagg 2581 gtttccggat gcgcaaacac cggcaggcaa aacgccgcaa aaaacggaat cagcgccaca 2641 cgaaaatgct gaatgctcat actcttcctt tttcaatatt attgaagcat ttatcagggt 2701 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggaa 2761 ttaaaaaaaa gcccgctcat taggcgggct actaatttaa atcgtaatta ttggggaccc 2821 ctggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat 2881 ggagttctga ggtcattact ggatctatca acaggagtcc aagactagtc gccagggttt 2941 tcccagtcac gacgcggccg cattgacggc tagctcagtc ctaggtacag tgctagctac 3001 tagagaaaga ggagaaatac tagatgagga atgacggagg ctttttgctg tggtgggacg 3061 gtttgcgtag cgagatgcag ccgatccacg acagccaggg cgtgttcgcc gtcctggaaa 3121 aggaagtgcg gcgcctgggc ttcgattact acgcctatgg cgtgcgccac acgattccct 3181 tcacccggcc gaagaccgag gtccatggca cctatcccaa ggcctggctg gagcgatacc 3241 agatgcagaa ctacggggcc gtggatccgg cgatcctcaa cggcctgcgc tcctcggaaa 3301 tggtggtctg gagcgacagc ctgttcgacc agagccggat gctctggaac gaggctcgcg 3361 attggggcct ctgtgtcggc gcgaccttgc cgatccgcgc gccgaacaat ttgctcagcg 3421 tgctttccgt ggcgcgcgac cagcagaaca tctccagctt cgagcgcgag gaaatccgcc 3481 tgcggctgcg ttgcatgatc gagttgctga cccagaagct gaccgacctg gagcatccga 3541 tgctgatgtc caacccggtc tgcctgagcc atcgcgaacg cgagatcctg caatggaccg 3601 ccgacggcaa gagttccggg gaaatcgcca tcatcctgag catctccgag agcacggtga 3661 acttccacca caagaacatc cagaagaagt tcgacgcgcc gaacaagacg ctggctgccg 3721 cctacgccgc ggcgctgggt ctcatctaat aatactagag ccaggcatca aataaaacga 3781 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 3841 tactagagtc acactggctc accttcgggt gggcctttct gcgtttataa ttaaaggcat 3901 caaataaaac //