LOCUS Exported 3924 bp ds-DNA circular SYN 10-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0042max_regulator_rhlR SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 3924) AUTHORS Daniel Gerngross TITLE piG0042max JOURNAL Exported 10 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3924 /organism="recombinant plasmid" /mol_type="other DNA" terminator 1..95 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" promoter 139..173 /note="const. promoter (J23100)" RBS 180..213 /note="Salis RBS Calculator, TIR=1541765.04" CDS 214..942 /codon_start=1 /product="RhlR (no LVA)" /note="rhlR (C0171)" /translation="MRNDGGFLLWWDGLRSEMQPIHDSQGVFAVLEKEVRRLGFDYYAY GVRHTIPFTRPKTEVHGTYPKAWLERYQMQNYGAVDPAILNGLRSSEMVVWSDSLFDQS RMLWNEARDWGLCVGATLPIRAPNNLLSVLSVARDQQNISSFEREEIRLRLRCMIELLT QKLTDLEHPMLMSNPVCLSHREREILQWTADGKSSGEIAIILSISESTVNFHHKNIQKK FDAPNKTLAAAYAAALGLI*" terminator 951..1079 /note="directional double term (B0015)" terminator 1091..1177 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" CDS 1359..1550 /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /note="rop" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" misc_feature 1652..1792 /note="bom" /note="basis of mobility region from pBR322" rep_origin complement(1963..2582) /direction=LEFT /note="pBR322_origin" oriT 2602..2710 /note="incP origin of transfer" CDS complement(2900..3760) /note="AmpR" promoter complement(3761..3852) /gene="bla" /note="AmpR promoter" ORIGIN 1 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag 61 ttctgaggtc attactggat ctatcaacag gagtccaaga ctagtcgcca gggttttccc 121 agtcacgacg cggccgcatt gacggctagc tcagtcctag gtacagtgct agctactaga 181 taaaagacgt aataagatat aaggaggtta ataatgagga atgacggagg ctttttgctg 241 tggtgggacg gtttgcgtag cgagatgcag ccgatccacg acagccaggg cgtgttcgcc 301 gtcctggaaa aggaagtgcg gcgcctgggc ttcgattact acgcctatgg cgtgcgccac 361 acgattccct tcacccggcc gaagaccgag gtccatggca cctatcccaa ggcctggctg 421 gagcgatacc agatgcagaa ctacggggcc gtggatccgg cgatcctcaa cggcctgcgc 481 tcctcggaaa tggtggtctg gagcgacagc ctgttcgacc agagccggat gctctggaac 541 gaggctcgcg attggggcct ctgtgtcggc gcgaccttgc cgatccgcgc gccgaacaat 601 ttgctcagcg tgctttccgt ggcgcgcgac cagcagaaca tctccagctt cgagcgcgag 661 gaaatccgcc tgcggctgcg ttgcatgatc gagttgctga cccagaagct gaccgacctg 721 gagcatccga tgctgatgtc caacccggtc tgcctgagcc atcgcgaacg cgagatcctg 781 caatggaccg ccgacggcaa gagttccggg gaaatcgcca tcatcctgag catctccgag 841 agcacggtga acttccacca caagaacatc cagaagaagt tcgacgcgcc gaacaagacg 901 ctggctgccg cctacgccgc ggcgctgggt ctcatctaat aatactagag ccaggcatca 961 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 1021 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttataa 1081 ttaaaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 1141 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cagctgggcg 1201 cgcccccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca 1261 tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc 1321 cccatgaaca gaaattcccc cttacacgga ggcatcaagt gaccaaacag gaaaaaaccg 1381 cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg 1441 agctggacgc ggatgaacag gcagacatct gtgaatcgct gcacgaccac gctgatgagc 1501 tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 1561 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 1621 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata 1681 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca 1741 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc 1801 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 1861 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 1921 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 1981 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 2041 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 2101 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 2161 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 2221 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 2281 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 2341 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 2401 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 2461 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 2521 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 2581 cttttctacg gggccggcct acggccagcc tcgcagagca ggattcccgt tgagcaccgc 2641 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc 2701 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca 2761 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc 2821 gaagcagggt tatgcagcgg aaaaggacaa gggtcgtcca aaaaaaaagg ctccaaaagg 2881 agcctttaat tgtatcggtt taccaatgtt taatcaggct cgcgccaatt tccgcaatct 2941 gacggttacg ttcatccatc gtcgcctggc tgccggtggt ataaatcacc acaatacggc 3001 tcggtttacc atccgggccc agcgccgcaa taatgccacg gctgccacgt tcgcccgcac 3061 cgcttttatc cgcaataaac cagccagccg gcagcgcgct acgcagcagc ggacccgcca 3121 ctttatccgc ttccatccaa tcaatcagtt gctggcggct tgccagggtc agcagctcac 3181 ccgtcagcag tttacgcagg gtggtcgcca ttgctgccgg catggtggta tcacgttcat 3241 cgttcggaat cgcttcgttc agttccggtt cccaacgatc cagacgggtc acatgatcgc 3301 ccatgttatg cagaaacgcg gtcagttctt tcggaccgcc aatggtcgtc agcagcaggt 3361 tcgccgcggt gttatcgctc atggtaatcg ccgcgctgca cagttcacgc acggtcatgc 3421 catcggtcag atgtttttcg gtcaccgggc tatattccac cagatcgttc tggctataat 3481 gaatacgacg gcccagttgt tcctggcccg catccacacg gctcagaacc gcaccgcaca 3541 gcagcacttt aaaggtgctc atcatcggaa aacgttcttc cggacgaaaa gattccagaa 3601 ttttgccgct gttcagatcc agttcaatat agcccacgcg cgcacccagt tgatcttccg 3661 catctttcac tttcaccagg gtttccggat gcgcaaacac cggcaggcaa aacgccgcaa 3721 aaaacggaat cagcgccaca cgaaaatgct gaatgctcat actcttcctt tttcaatatt 3781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 3841 aaaataaaca aataggggaa ttaaaaaaaa gcccgctcat taggcgggct actaatttaa 3901 atcgtaatta ttggggaccc ctgg //