LOCUS Exported 3937 bp ds-DNA circular SYN 10-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0046_regulator_luxR(J23109) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3937) AUTHORS Daniel Gerngross TITLE piG0046 JOURNAL Exported 10 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3937 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 12..98 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" rep_origin 135..754 /direction=RIGHT /note="pBR322_origin" misc_feature 925..1065 /note="bom" /note="basis of mobility region from pBR322" misc_feature complement(1167..1358) /note="ROP" oriT 1523..1631 /note="incP origin of transfer" CDS complement(1821..2681) /note="AmpR" promoter complement(2682..2773) /gene="bla" /note="AmpR promoter" terminator 2820..2945 /note="T0" promoter 2984..3018 /note="const. promoter (J23100)" RBS 3027..3038 /note="RBS (B0034)" CDS 3045..3800 /codon_start=1 /product="LuxR-Barcode" /note="luxR (C0062)" /translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLAI IYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAVNK KSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPLIVP SLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHLTNAQ MKLNTTNRCQSISKAILTGAIDCPYFKN*" terminator 3809..3937 /note="directional double term (B0015)" ORIGIN 1 attaaaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 61 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acagctgggc 121 gcgccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 181 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 241 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 301 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 361 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 421 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 481 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 541 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 601 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 661 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 721 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 781 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 841 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 901 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg 961 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag 1021 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca 1081 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 1141 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 1201 aggcagctgc ggtaaagctc atcagcgtgg tcgtgcagcg attcacagat gtctgcctgt 1261 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag 1321 cgggccatgt taagggcggt tttttcctgt ttggtcactt gatgcctccg tgtaaggggg 1381 aatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg 1441 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat 1501 ggatgcggcg ggggccggcc tacggccagc ctcgcagagc aggattcccg ttgagcaccg 1561 ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac 1621 ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc 1681 aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc 1741 cgaagcaggg ttatgcagcg gaaaaggaca agggtcgtcc aaaaaaaaag gctccaaaag 1801 gagcctttaa ttgtatcggt ttaccaatgt ttaatcaggc tcgcgccaat ttccgcaatc 1861 tgacggttac gttcatccat cgtcgcctgg ctgccggtgg tataaatcac cacaatacgg 1921 ctcggtttac catccgggcc cagcgccgca ataatgccac ggctgccacg ttcgcccgca 1981 ccgcttttat ccgcaataaa ccagccagcc ggcagcgcgc tacgcagcag cggacccgcc 2041 actttatccg cttccatcca atcaatcagt tgctggcggc ttgccagggt cagcagctca 2101 cccgtcagca gtttacgcag ggtggtcgcc attgctgccg gcatggtggt atcacgttca 2161 tcgttcggaa tcgcttcgtt cagttccggt tcccaacgat ccagacgggt cacatgatcg 2221 cccatgttat gcagaaacgc ggtcagttct ttcggaccgc caatggtcgt cagcagcagg 2281 ttcgccgcgg tgttatcgct catggtaatc gccgcgctgc acagttcacg cacggtcatg 2341 ccatcggtca gatgtttttc ggtcaccggg ctatattcca ccagatcgtt ctggctataa 2401 tgaatacgac ggcccagttg ttcctggccc gcatccacac ggctcagaac cgcaccgcac 2461 agcagcactt taaaggtgct catcatcgga aaacgttctt ccggacgaaa agattccaga 2521 attttgccgc tgttcagatc cagttcaata tagcccacgc gcgcacccag ttgatcttcc 2581 gcatctttca ctttcaccag ggtttccgga tgcgcaaaca ccggcaggca aaacgccgca 2641 aaaaacggaa tcagcgccac acgaaaatgc tgaatgctca tactcttcct ttttcaatat 2701 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 2761 aaaaataaac aaatagggga attaaaaaaa agcccgctca ttaggcgggc tactaattta 2821 aatcgtaatt attggggacc cctggattct caccaataaa aaacgcccgg cggcaaccga 2881 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc 2941 caagactagt cgccagggtt ttcccagtca cgacgcggcc gcatttacag ctagctcagt 3001 cctagggact gtgctagcta ctagagaaag aggagaaata ctagatgaaa aacataaatg 3061 ccgacgacac atacagaata attaataaaa ttaaagcttg tagaagcaat aatgatatta 3121 atcaatgctt atctgatatg actaaaatgg tacattgtga atattattta ctcgcgatca 3181 tttatcctca ttctatggtt aaatctgata tttcaatcct agataattac cctaaaaaat 3241 ggaggcaata ttatgatgac gctaatttaa taaaatatga tcctatagta gattattcta 3301 actccaatca ttcaccaatt aattggaata tatttgaaaa caatgctgta aataaaaaat 3361 ctccaaatgt aattaaagaa gcgaaaacat caggtcttat cactgggttt agtttcccta 3421 ttcatacggc taacaatggc ttcggaatgc ttagttttgc acattcagaa aaagacaact 3481 atatagatag tttattttta catgcgtgta tgaacatacc attaattgtt ccttctctag 3541 ttgataatta tcgaaaaata aatatagcaa ataataaatc aaacaacgat ttaaccaaaa 3601 gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat atttcaaaaa 3661 tattaggttg cagtgagcgt actgtcactt tccatttaac caatgcgcaa atgaaactca 3721 atacaacaaa ccgctgccaa agtatttcta aagcaatttt aacaggagca attgattgcc 3781 catactttaa aaattaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 3841 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 3901 actggctcac cttcgggtgg gcctttctgc gtttata //