LOCUS Exported 3682 bp ds-DNA circular SYN 17-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0058_sensor_plasRstRBS-sfGFP SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 3682) AUTHORS Daniel Gerngross TITLE Direct Submission JOURNAL Exported 17 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3682 /organism="recombinant plasmid" /mol_type="other DNA" terminator 1..126 /note="T0" terminator 27..121 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" misc_feature 127..196 /note="spacer (B0040)" promoter 197..353 /note="P(LasR) (R0079)" RBS 362..373 /note="RBS (B0034)" CDS 380..1105 /codon_start=1 /note="sfGFP" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYKRP*" terminator 1108..1224 /note="T1" terminator 1117..1203 /gene="Escherichia coli rrnB" /note="T1" /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 1231..1250 /note="AB179" primer_bind 1416..1435 /note="AB5" rep_origin 1569..2396 /direction=RIGHT /note="p15A" rep_origin 1817..1819 /direction=RIGHT /note="P15 ori from pACYC184" rep_origin complement(1820..2364) /direction=LEFT /note="p15A ori" /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." primer_bind 2468..2487 /note="AB180" oriT 2498..2606 /note="incP origin of transfer" CDS complement(2762..3577) /note="KamR" ORIGIN 1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg 61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 121 ccaagaaggt tctgttaagt aactgaaccc aatgtcgtta gtgacgctta cctcttaaga 181 ggtcactgac ctaacagccc ctcgctgagc gcgtcccgga gctgggggca acctagctgc 241 cacctgcttt tctgctagct attccagcga aaacatacag atttccggcg aaatcaaggc 301 tacctgccag ttctggcagg tttggccgcg ggttcttttt ggtacacgaa agctactaga 361 gaaagaggag aaatactaga tgagcaaagg agaagaactt ttcactggag ttgtcccaat 421 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtccgtg gagagggtga 481 aggtgatgct acaaacggaa aactcaccct taaatttatt tgcactactg gaaaactacc 541 tgttccgtgg ccaacacttg tcactactct gacctatggt gttcaatgct tttcccgtta 601 tccggatcac atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca 661 ggaacgcact atatctttca aagatgacgg gacctacaag acgcgtgctg aagtcaagtt 721 tgaaggtgat acccttgtta atcgtatcga gttaaagggt attgatttta aagaagatgg 781 aaacattctt ggacacaaac tcgagtacaa ctttaactca cacaatgtat acatcacggc 841 agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt cgccacaacg ttgaagatgg 901 ttccgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct 961 tttaccagac aaccattacc tgtcgacaca atctgtcctt tcgaaagatc ccaacgaaaa 1021 gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac atggcatgga 1081 tgagctctac aaaaggcctt aataaattaa aggcatcaaa taaaacgaaa ggctcagtcg 1141 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca 1201 aatccgccgc cctagacagc tgggcgcgcc aaatacgccc ggtagtgatc ttatttcatt 1261 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc 1321 ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc 1381 cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact tactgattta 1441 gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct 1501 gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctagc 1561 ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt 1621 ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc 1681 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct 1741 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga 1801 gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct 1861 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc 1921 tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct 1981 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct 2041 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta 2101 actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg 2161 gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg 2221 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct 2281 cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt 2341 acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa atatttctag 2401 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 2461 atttccccga aaagtgccac ctgctagggc cggcctacgg ccagcctcgc agagcaggat 2521 tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg 2581 gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta 2641 cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa 2701 tcgctataat gaccccgaag cagggttatg cagcggaaaa ggacccttgt ccaattaatt 2761 attagaaaaa ttcatccagc atcagatgaa attgcagttt gttcatatcc ggattatcaa 2821 tgccatattt ctgaaacaga cgtttttgca ggctcgggct aaattcgccc aggcagttcc 2881 acagaatggc cagatcctga taacgatccg caatgcccac acggcccaca tcaatgcagc 2941 caatcagttt gccttcatcg aaaatcaggt tatccaggct aaaatcgccg tgggtcacca 3001 cgctatccgg gctaaacggc agcagtttat gcatttcttt ccacacctgt tccaccggcc 3061 agccgttacg ttcatcatca aaatcgctcg catccaccag gccgttgttc atacggctct 3121 gcgcctgggc cagacgaaac acacgatcgc tgttaaacgg gcagttgcac accggaatgc 3181 tatgcagacg acgcagaaac acggccagcg catccacaat gttttcgccg ctatccggat 3241 attcttccag cacctgaaac gcggttttgc ccggaatcgc ggtggtcagc agccacgcat 3301 catccggggt gcgaataaaa tgtttaatgg tcggcagcgg cataaattcg gtcagccagt 3361 tcagacgcac catttcatcg gtcacatcgt tcgccacgct gcctttgcca tgtttcagaa 3421 acagttccgg cgcatccggt ttgccataca gacgataaat ggtcgcgccg ctctgaccca 3481 cgttatcacg cgcccattta tagccataca gatccgcatc catgttgctg ttcagacgcg 3541 gacggctaca gctcgtttca cgctgaatat ggctcataac accccttgta ttactgttta 3601 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc 3661 agagattttg agacacaaat tt //