LOCUS Exported 4863 bp ds-DNA circular SYN 10-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0043_regulator_lasR-luxR_new3 SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 4863) AUTHORS Daniel Gerngross TITLE piG0043 JOURNAL Exported 10 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4863 /organism="recombinant plasmid" /mol_type="other DNA" misc_feature 11..202 /note="ROP" misc_feature 304..444 /note="bom" /note="basis of mobility region from pBR322" rep_origin complement(615..1234) /direction=LEFT /note="pBR322_origin" oriT 1254..1362 /note="incP origin of transfer" CDS complement(1552..2412) /note="AmpR" promoter complement(2413..2504) /gene="bla" /note="AmpR promoter" terminator 2577..2671 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" promoter 2715..2749 /note="const. promoter (J23100)" RBS 2758..2769 /note="RBS (B0034)" CDS 2776..3498 /codon_start=1 /note="lasR (C0179)" /translation="MALVDGFLELERSSGKLEWSAILQKMASDLGFSKILFGLLPKDSQ DYENAFIVGNYPAAWREHYDRAGYARVDPTVSHCTQSVLPIFWEPSIYQTRKQHEFFEE ASAAGLVYGLTMPLHGARGELGALSLSVEAENRAEANRFIESVLPTLWMLKDYALQSGA GLAFEHPVSKPVVLTSREKEVLQWCAIGKTSWEISVICNCSEANVNFHMGNIRRKFGVT SRRVAAIMAVNLGLITL*" terminator 3507..3635 /note="directional double term (B0015)" promoter 3641..3675 /note="const. promoter (J23100)" RBS 3684..3695 /note="RBS (B0034)" CDS 3702..4457 /codon_start=1 /product="LuxR-Barcode" /note="luxR (C0062)" /translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLAI IYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAVNK KSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPLIVP SLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHLTNAQ MKLNTTNRCQSISKAILTGAIDCPYFKN*" terminator 4466..4594 /note="directional double term (B0015)" terminator 4606..4692 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 gaggcatcaa gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag 61 ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat 121 ctgtgaatcg ctgcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg 181 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 241 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 301 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg 361 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc 421 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc 481 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 541 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 601 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 661 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 721 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 781 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 841 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 901 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 961 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 1021 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 1081 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 1141 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 1201 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggccggc ctacggccag 1261 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag 1321 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata 1381 caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat 1441 ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaaggac 1501 aagggtcgtc caaaaaaaaa ggctccaaaa ggagccttta attgtatcgg tttaccaatg 1561 tttaatcagg ctcgcgccaa tttccgcaat ctgacggtta cgttcatcca tcgtcgcctg 1621 gctgccggtg gtataaatca ccacaatacg gctcggttta ccatccgggc ccagcgccgc 1681 aataatgcca cggctgccac gttcgcccgc accgctttta tccgcaataa accagccagc 1741 cggcagcgcg ctacgcagca gcggacccgc cactttatcc gcttccatcc aatcaatcag 1801 ttgctggcgg cttgccaggg tcagcagctc acccgtcagc agtttacgca gggtggtcgc 1861 cattgctgcc ggcatggtgg tatcacgttc atcgttcgga atcgcttcgt tcagttccgg 1921 ttcccaacga tccagacggg tcacatgatc gcccatgtta tgcagaaacg cggtcagttc 1981 tttcggaccg ccaatggtcg tcagcagcag gttcgccgcg gtgttatcgc tcatggtaat 2041 cgccgcgctg cacagttcac gcacggtcat gccatcggtc agatgttttt cggtcaccgg 2101 gctatattcc accagatcgt tctggctata atgaatacga cggcccagtt gttcctggcc 2161 cgcatccaca cggctcagaa ccgcaccgca cagcagcact ttaaaggtgc tcatcatcgg 2221 aaaacgttct tccggacgaa aagattccag aattttgccg ctgttcagat ccagttcaat 2281 atagcccacg cgcgcaccca gttgatcttc cgcatctttc actttcacca gggtttccgg 2341 atgcgcaaac accggcaggc aaaacgccgc aaaaaacgga atcagcgcca cacgaaaatg 2401 ctgaatgctc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 2461 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg aattaaaaaa 2521 aagcccgctc attaggcggg ctactaattt aaatcgtaat tattggggac ccctggattc 2581 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct 2641 gaggtcatta ctggatctat caacaggagt ccaagactag tcgccagggt tttcccagtc 2701 acgacgcggc cgcattgacg gctagctcag tcctaggtac agtgctagct actagagaaa 2761 gaggagaaat actagatggc cttggttgac ggttttcttg agctggaacg ctcaagtgga 2821 aaattggagt ggagcgccat cctccagaag atggcgagcg accttggatt ctcgaagatc 2881 ctgttcggcc tgttgcctaa ggacagccag gactacgaga acgccttcat cgtcggcaac 2941 tacccggccg cctggcgcga gcattacgac cgggctggct acgcgcgggt cgacccgacg 3001 gtcagtcact gtacccagag cgtactgccg attttctggg aaccgtccat ctaccagacg 3061 cgaaagcagc acgagttctt cgaggaagcc tcggccgccg gcctggtgta tgggctgacc 3121 atgccgctgc atggtgctcg cggcgaactc ggcgcgctga gcctcagcgt ggaagcggaa 3181 aaccgggccg aggccaaccg tttcatagag tcggtcctgc cgaccctgtg gatgctcaag 3241 gactacgcac tgcaaagcgg tgccggactg gccttcgaac atccggtcag caaaccggtg 3301 gttctgacca gccgggagaa ggaagtgttg cagtggtgcg ccatcggcaa gaccagttgg 3361 gagatatcgg ttatctgcaa ctgctcggaa gccaatgtga acttccatat gggaaatatt 3421 cggcggaagt tcggtgtgac ctcccgccgc gtagcggcca ttatggccgt taatttgggt 3481 cttattactc tctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 3541 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 3601 tggctcacct tcgggtgggc ctttctgcgt ttataattaa ttgacggcta gctcagtcct 3661 aggtacagtg ctagctacta gagaaagagg agaaatacta gatgaaaaac ataaatgccg 3721 acgacacata cagaataatt aataaaatta aagcttgtag aagcaataat gatattaatc 3781 aatgcttatc tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt 3841 atcctcattc tatggttaaa tctgatattt caatcctaga taattaccct aaaaaatgga 3901 ggcaatatta tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact 3961 ccaatcattc accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc 4021 caaatgtaat taaagaagcg aaaacatcag gtcttatcac tgggtttagt ttccctattc 4081 atacggctaa caatggcttc ggaatgctta gttttgcaca ttcagaaaaa gacaactata 4141 tagatagttt atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg 4201 ataattatcg aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag 4261 aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat 4321 taggttgcag tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata 4381 caacaaaccg ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat 4441 actttaaaaa ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 4501 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 4561 ggctcacctt cgggtgggcc tttctgcgtt tataattaaa ggcatcaaat aaaacgaaag 4621 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg 4681 agtaggacaa atccgccgcc ctagacagct gggcgcgccc ccgccgcatc cataccgcca 4741 gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg 4801 tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat tcccccttac 4861 acg //