{"x/pBknz7fTo99c5GX85":{"params":{"87f8a6ab85c9ced3702b4ea641ad4bb5":{"v":1,"experiment":"aJkHPGccpLkiga4w5","text":"Lysogeny broth + ","type":"experiment","product":"Medium"},"4c0984e0a4d80995f3099e5c33188ce1":{"experiment":"KCeR3NwfmpW74HoSj","v":0,"type":"experiment","text":"E. coli BL21(DE3) with pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3","product":"Glycerol stock"}},"protocol":{"_id":"9TiKPrpdykEiSHFcy","v":5,"name":"Make a liquid culture carrying a plasmid"},"values":{"1":{"0":"37","1":0},"0":{"1":"K","0":""}},"finishDate":1412182800,"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"allTypes":[{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"},{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3}],"text":"E. coli BL21(DE3) with pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3","type":{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3},"properties":{"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)"},"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3","10990dcf66373fff11ef907bc2b1ef97":"K"}}}}},"x/EHXMQtaWg29qNjupk":{"protocol":{"name":"Make a liquid culture carrying a plasmid","v":5,"_id":"9TiKPrpdykEiSHFcy"},"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"product":"Glycerol stock","text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+may1","type":"experiment","experiment":"oRLoLYGWwMFm84ebL","v":0},"87f8a6ab85c9ced3702b4ea641ad4bb5":{"experiment":"aJkHPGccpLkiga4w5","v":1,"text":"Lysogeny broth + ","type":"experiment","product":"Medium"}},"finishDate":1412182800,"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+may1","10990dcf66373fff11ef907bc2b1ef97":"K"},"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)","e1520b5997a532c7889f6e8883920ab8":"E. coli"}},"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+may1","type":{"_id":"EZz9pqmLYbZXhdQuZ","v":3,"name":"Bacterial culture carrying a plasmid"},"allTypes":[{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"},{"_id":"YW7epnToTmqmHEEwx","name":"Bacteria","v":0},{"name":"Bacterial culture carrying a plasmid","v":3,"_id":"EZz9pqmLYbZXhdQuZ"}]}},"values":{"0":{"1":"K","0":""},"1":{"1":0,"0":"37"}}},"x/TXKcwtrzPf9m8XZMM":{"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+sgt2","10990dcf66373fff11ef907bc2b1ef97":"K"}},"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2},{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"EZz9pqmLYbZXhdQuZ","v":3,"name":"Bacterial culture carrying a plasmid"}],"type":{"v":3,"name":"Bacterial culture carrying a plasmid","_id":"EZz9pqmLYbZXhdQuZ"},"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+sgt2"}},"finishDate":1412182800,"values":{"0":{"0":"","1":"K"},"1":{"0":"37","1":0}},"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"type":"experiment","text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+sgt2","experiment":"YnPT5MFPE7SXPg5sA","v":0,"product":"Glycerol stock"},"87f8a6ab85c9ced3702b4ea641ad4bb5":{"product":"Medium","v":1,"experiment":"aJkHPGccpLkiga4w5","type":"experiment","text":"Lysogeny broth + "}},"protocol":{"name":"Make a liquid culture carrying a plasmid","v":5,"_id":"9TiKPrpdykEiSHFcy"}},"x/dXYPp3o9wnSA6q5wj":{"values":{"0":{"1":"K","0":""},"1":{"0":"37","1":0}},"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+sho1","10990dcf66373fff11ef907bc2b1ef97":"K"}},"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2},{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3}],"type":{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3},"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+sho1"}},"finishDate":1412182800,"params":{"87f8a6ab85c9ced3702b4ea641ad4bb5":{"v":1,"experiment":"aJkHPGccpLkiga4w5","type":"experiment","text":"Lysogeny broth + ","product":"Medium"},"4c0984e0a4d80995f3099e5c33188ce1":{"product":"Glycerol stock","v":0,"experiment":"XLZkfeRqzZroqei37","text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+sho1","type":"experiment"}},"protocol":{"_id":"9TiKPrpdykEiSHFcy","v":5,"name":"Make a liquid culture carrying a plasmid"}},"x/cXWBtY2bdHxBeGuML":{"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"product":"Glycerol stock","experiment":"cpxFbJ8EbmkyfD7du","v":0,"type":"experiment","text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+sho2"},"87f8a6ab85c9ced3702b4ea641ad4bb5":{"experiment":"aJkHPGccpLkiga4w5","v":1,"type":"experiment","text":"Lysogeny broth + ","product":"Medium"}},"protocol":{"_id":"9TiKPrpdykEiSHFcy","v":5,"name":"Make a liquid culture carrying a plasmid"},"finishDate":1412182800,"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2},{"_id":"YW7epnToTmqmHEEwx","name":"Bacteria","v":0},{"_id":"EZz9pqmLYbZXhdQuZ","v":3,"name":"Bacterial culture carrying a plasmid"}],"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+sho2","type":{"v":3,"name":"Bacterial culture carrying a plasmid","_id":"EZz9pqmLYbZXhdQuZ"},"properties":{"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"K","0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+sho2"},"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)"}}}},"values":{"0":{"0":"","1":"K"},"1":{"0":"37","1":0}}},"x/u6WZe2Afj8k4Ko79g":{"values":{"1":{"1":0,"0":"37"},"0":{"0":"","1":"K"}},"finishDate":1412182800,"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"allTypes":[{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"},{"name":"Bacteria","v":0,"_id":"YW7epnToTmqmHEEwx"},{"name":"Bacterial culture carrying a plasmid","v":3,"_id":"EZz9pqmLYbZXhdQuZ"}],"type":{"v":3,"name":"Bacterial culture carrying a plasmid","_id":"EZz9pqmLYbZXhdQuZ"},"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+flexlinker","properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+flexlinker","10990dcf66373fff11ef907bc2b1ef97":"K"}}}},"protocol":{"name":"Make a liquid culture carrying a plasmid","v":5,"_id":"9TiKPrpdykEiSHFcy"},"params":{"87f8a6ab85c9ced3702b4ea641ad4bb5":{"product":"Medium","v":1,"experiment":"aJkHPGccpLkiga4w5","text":"Lysogeny broth + ","type":"experiment"},"4c0984e0a4d80995f3099e5c33188ce1":{"experiment":"S3jcXxDzAAxzWjJ8F","v":0,"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+flexlinker","type":"experiment","product":"Glycerol stock"}}},"x/YnFuR29JeiymkDfNk":{"protocol":{"_id":"kxBySpW3SnbWuYMRw","name":"Streak bacteria carrying a plasmid onto an agar plate","v":0},"params":{"a1610acb29b294b95abcdeb4b9fdd74b":{"experiment":"pXGBuCaP3iEycBBtj","v":0,"type":"experiment","text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","product":"Bacteria carrying the plasmid"}},"finishDate":1412194500,"products":{"fccc3bbdd62f7ea544bf80e802c28b89":{"text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","type":{"v":0,"name":"Agar plate with bacteria carrying a plasmid","_id":"D2DD2v2FYpYQLFwji"},"allTypes":[{"_id":"D2DD2v2FYpYQLFwji","name":"Agar plate with bacteria carrying a plasmid","v":0}],"properties":{"D2DD2v2FYpYQLFwji":{"1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","0ac71517d2d368cdbabf8b9914716902":"pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","e1520b5997a532c7889f6e8883920ab8":"E. coli","e4963ca4d4576f5cb6f8af52e3d038b6":"C"}}}},"values":{"0":{"1":"C","0":""}}},"x/zWzMnbbH8dCgkKnHN":{"params":{"a1610acb29b294b95abcdeb4b9fdd74b":{"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)","type":"experiment","experiment":"9AcsE7FwRmtKqR3zz","v":0,"product":"Bacteria carrying the plasmid"}},"protocol":{"_id":"kxBySpW3SnbWuYMRw","v":0,"name":"Streak bacteria carrying a plasmid onto an agar plate"},"values":{"0":{"1":"C","0":""}},"products":{"fccc3bbdd62f7ea544bf80e802c28b89":{"properties":{"D2DD2v2FYpYQLFwji":{"e4963ca4d4576f5cb6f8af52e3d038b6":"C","e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","0ac71517d2d368cdbabf8b9914716902":"pSB1C3-2Zv2Npu:(LambdaLys-H6)"}},"allTypes":[{"v":0,"name":"Agar plate with bacteria carrying a plasmid","_id":"D2DD2v2FYpYQLFwji"}],"type":{"_id":"D2DD2v2FYpYQLFwji","v":0,"name":"Agar plate with bacteria carrying a plasmid"},"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)"}},"finishDate":1412194500},"x/ZPDxo2FFnE6jNgNEs":{"protocol":{"_id":"kxBySpW3SnbWuYMRw","v":0,"name":"Streak bacteria carrying a plasmid onto an agar plate"},"params":{"a1610acb29b294b95abcdeb4b9fdd74b":{"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-Rigid)","type":"experiment","v":0,"experiment":"L2TSxEqwm9nWYPM7K","product":"Bacteria carrying the plasmid"}},"values":{"0":{"0":"","1":"C"}},"finishDate":1412194500,"products":{"fccc3bbdd62f7ea544bf80e802c28b89":{"properties":{"D2DD2v2FYpYQLFwji":{"0ac71517d2d368cdbabf8b9914716902":"pSB1C3-2Zv2Npu:(LambdaLys-Rigid)","1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e4963ca4d4576f5cb6f8af52e3d038b6":"C","e1520b5997a532c7889f6e8883920ab8":"E. coli"}},"allTypes":[{"_id":"D2DD2v2FYpYQLFwji","v":0,"name":"Agar plate with bacteria carrying a plasmid"}],"type":{"_id":"D2DD2v2FYpYQLFwji","v":0,"name":"Agar plate with bacteria carrying a plasmid"},"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-Rigid)"}}},"x/acoW4M3QEL98pFbL2":{"params":{"a1610acb29b294b95abcdeb4b9fdd74b":{"product":"Bacteria carrying the plasmid","text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord1","type":"experiment","v":0,"experiment":"Jp3KfgTXnixZ59EWT"}},"protocol":{"_id":"kxBySpW3SnbWuYMRw","v":0,"name":"Streak bacteria carrying a plasmid onto an agar plate"},"values":{"0":{"0":"","1":"K"}},"finishDate":1412194500,"products":{"fccc3bbdd62f7ea544bf80e802c28b89":{"properties":{"D2DD2v2FYpYQLFwji":{"e4963ca4d4576f5cb6f8af52e3d038b6":"K","e1520b5997a532c7889f6e8883920ab8":"E. coli","0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+ord1","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)"}},"allTypes":[{"_id":"D2DD2v2FYpYQLFwji","name":"Agar plate with bacteria carrying a plasmid","v":0}],"type":{"name":"Agar plate with bacteria carrying a plasmid","v":0,"_id":"D2DD2v2FYpYQLFwji"},"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord1"}}},"x/BAL4hfMeRWZKv4kBP":{"protocol":{"_id":"kxBySpW3SnbWuYMRw","name":"Streak bacteria carrying a plasmid onto an agar plate","v":0},"params":{"a1610acb29b294b95abcdeb4b9fdd74b":{"product":"Bacteria carrying the plasmid","text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord3","type":"experiment","v":0,"experiment":"XvAFdd6cC8jdpxLD4"}},"values":{"0":{"0":"","1":"K"}},"products":{"fccc3bbdd62f7ea544bf80e802c28b89":{"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord3","type":{"name":"Agar plate with bacteria carrying a plasmid","v":0,"_id":"D2DD2v2FYpYQLFwji"},"allTypes":[{"_id":"D2DD2v2FYpYQLFwji","v":0,"name":"Agar plate with bacteria carrying a plasmid"}],"properties":{"D2DD2v2FYpYQLFwji":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+ord3","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)","e1520b5997a532c7889f6e8883920ab8":"E. coli","e4963ca4d4576f5cb6f8af52e3d038b6":"K"}}}},"finishDate":1412194500},"x/uev4t7NuoSpaYRnma":{"finishDate":1412194500,"products":{"1f90a3d695361444cb4d5bd0bc04c741":{"properties":{"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSB1C3-LiLaLy(H6)","10990dcf66373fff11ef907bc2b1ef97":"C"},"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo"}},"text":"E. coli Turbo with pSB1C3-LiLaLy(H6)","type":{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2},"allTypes":[{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2}]}},"values":{"0":{"0":""},"6":{"1":"","2":37,"0":""},"8":{"0":""},"4":{"0":""},"5":{"0":""},"2":{"0":""},"3":{"0":""}},"params":{"63aa3271e10f79baaba8465140e427e9":{"text":"pSB1C3-LiLaLy(H6) (S361)","type":"experiment","experiment":"wcGNzMugiz6HSp6cx","v":0,"product":"Plasmid"},"691586db4be0d1aff3ffb8b43b31cc9a":{"experiment":"DWbyZ2rdvbmNS7PKa","v":0,"type":"experiment","text":"E. coli Turbo","product":"Competent cells"}},"protocol":{"name":"Heat shock plasmid transformation (0 -> 1 plasmid)","v":4,"_id":"mJNyMCoEko3JKRv5x"}},"x/HePzLpnheaY9LCrRc":{"finishDate":1412194500,"products":{"fccc3bbdd62f7ea544bf80e802c28b89":{"properties":{"D2DD2v2FYpYQLFwji":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","e4963ca4d4576f5cb6f8af52e3d038b6":"C","0ac71517d2d368cdbabf8b9914716902":"pSB1C3-LiLaLy(H6)","1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo"}},"allTypes":[{"_id":"D2DD2v2FYpYQLFwji","v":0,"name":"Agar plate with bacteria carrying a plasmid"}],"text":"E. coli Turbo with pSB1C3-LiLaLy(H6)","type":{"_id":"D2DD2v2FYpYQLFwji","v":0,"name":"Agar plate with bacteria carrying a plasmid"}}},"values":{"0":{"1":"C","0":""}},"protocol":{"_id":"kxBySpW3SnbWuYMRw","name":"Streak bacteria carrying a plasmid onto an agar plate","v":0},"params":{"a1610acb29b294b95abcdeb4b9fdd74b":{"text":"E. coli Turbo with pSB1C3-LiLaLy(H6)","type":"experiment","experiment":"uev4t7NuoSpaYRnma","v":0,"product":"Bacteria carrying the plasmid"}}},"x/Jp3KfgTXnixZ59EWT":{"values":{"0":{"0":""},"4":{"0":""},"6":{"1":"","2":37,"0":""},"8":{"0":""},"2":{"0":""},"3":{"0":""},"5":{"0":""}},"products":{"1f90a3d695361444cb4d5bd0bc04c741":{"allTypes":[{"_id":"YW7epnToTmqmHEEwx","name":"Bacteria","v":0},{"name":"Bacteria carrying a plasmid","v":2,"_id":"PpBkfrLAqTw2CwNZX"}],"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord1","type":{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2},"properties":{"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+ord1","10990dcf66373fff11ef907bc2b1ef97":"K"},"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)"}}}},"finishDate":1412194500,"protocol":{"v":4,"name":"Heat shock plasmid transformation (0 -> 1 plasmid)","_id":"mJNyMCoEko3JKRv5x"},"params":{"691586db4be0d1aff3ffb8b43b31cc9a":{"v":0,"experiment":"YoYD8ZJSHdSzLY8pH","text":"E. coli BL21(DE3)","type":"experiment","product":"Competent cells"},"63aa3271e10f79baaba8465140e427e9":{"text":"pSBX1K3-2Zv2Npu:LaLys+ord1 (O296-2)","type":"experiment","v":0,"experiment":"WW87FdnkHz5LScfDt","product":"Purified plasmid"}}},"x/XvAFdd6cC8jdpxLD4":{"values":{"0":{"0":""},"5":{"0":""},"3":{"0":""},"2":{"0":""},"6":{"1":"","2":37,"0":""},"8":{"0":""},"4":{"0":""}},"products":{"1f90a3d695361444cb4d5bd0bc04c741":{"properties":{"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"K","0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+ord3"},"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)"}},"allTypes":[{"_id":"YW7epnToTmqmHEEwx","name":"Bacteria","v":0},{"_id":"PpBkfrLAqTw2CwNZX","v":2,"name":"Bacteria carrying a plasmid"}],"type":{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"},"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord3"}},"finishDate":1412194500,"params":{"691586db4be0d1aff3ffb8b43b31cc9a":{"product":"Competent cells","type":"experiment","text":"E. coli BL21(DE3)","v":0,"experiment":"YoYD8ZJSHdSzLY8pH"},"63aa3271e10f79baaba8465140e427e9":{"product":"Purified plasmid","experiment":"N3LTKdGqfMnKpCKYj","v":0,"text":"pSBX1K3-2Zv2Npu:LaLys+ord3 (O298)","type":"experiment"}},"protocol":{"name":"Heat shock plasmid transformation (0 -> 1 plasmid)","v":4,"_id":"mJNyMCoEko3JKRv5x"}},"x/pXGBuCaP3iEycBBtj":{"finishDate":1412194500,"products":{"1f90a3d695361444cb4d5bd0bc04c741":{"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","10990dcf66373fff11ef907bc2b1ef97":"C"}},"allTypes":[{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"}],"text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","type":{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"}}},"values":{"4":{"0":""},"8":{"0":""},"6":{"0":"","2":37,"1":""},"3":{"0":""},"2":{"0":""},"5":{"0":""},"0":{"0":""}},"protocol":{"_id":"mJNyMCoEko3JKRv5x","v":4,"name":"Heat shock plasmid transformation (0 -> 1 plasmid)"},"params":{"63aa3271e10f79baaba8465140e427e9":{"experiment":"wdjAw86qvjMxqs7Af","v":0,"text":"pSB1C3-T7RBS-LambdaLys-IGEMHD_v3 (S348)","type":"experiment","product":"Plasmid"},"691586db4be0d1aff3ffb8b43b31cc9a":{"type":"experiment","text":"E. coli Turbo","v":0,"experiment":"DWbyZ2rdvbmNS7PKa","product":"Competent cells"}}},"x/L2TSxEqwm9nWYPM7K":{"protocol":{"_id":"mJNyMCoEko3JKRv5x","v":4,"name":"Heat shock plasmid transformation (0 -> 1 plasmid)"},"params":{"691586db4be0d1aff3ffb8b43b31cc9a":{"product":"Competent cells","v":0,"experiment":"DWbyZ2rdvbmNS7PKa","text":"E. coli Turbo","type":"experiment"},"63aa3271e10f79baaba8465140e427e9":{"type":"experiment","text":"pSB1C3-2Zv2Npu:(LambdaLys-Rigid) (S353)","v":0,"experiment":"25i8R9Btg79q9p3Sr","product":"Plasmid"}},"finishDate":1412194500,"products":{"1f90a3d695361444cb4d5bd0bc04c741":{"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"C","0ac71517d2d368cdbabf8b9914716902":"pSB1C3-2Zv2Npu:(LambdaLys-Rigid)"}},"allTypes":[{"_id":"YW7epnToTmqmHEEwx","name":"Bacteria","v":0},{"_id":"PpBkfrLAqTw2CwNZX","v":2,"name":"Bacteria carrying a plasmid"}],"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-Rigid)","type":{"name":"Bacteria carrying a plasmid","v":2,"_id":"PpBkfrLAqTw2CwNZX"}}},"values":{"0":{"0":""},"2":{"0":""},"3":{"0":""},"5":{"0":""},"4":{"0":""},"8":{"0":""},"6":{"1":"","2":37,"0":""}}},"x/9AcsE7FwRmtKqR3zz":{"finishDate":1412194500,"products":{"1f90a3d695361444cb4d5bd0bc04c741":{"properties":{"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo"},"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"C","0ac71517d2d368cdbabf8b9914716902":"pSB1C3-2Zv2Npu:(LambdaLys-H6)"}},"allTypes":[{"_id":"YW7epnToTmqmHEEwx","name":"Bacteria","v":0},{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2}],"type":{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"},"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)"}},"values":{"3":{"0":""},"2":{"0":""},"5":{"0":""},"4":{"0":""},"8":{"0":""},"6":{"1":"","2":37,"0":""},"0":{"0":""}},"params":{"691586db4be0d1aff3ffb8b43b31cc9a":{"v":0,"experiment":"DWbyZ2rdvbmNS7PKa","text":"E. coli Turbo","type":"experiment","product":"Competent cells"},"63aa3271e10f79baaba8465140e427e9":{"type":"experiment","text":"pSB1C3-2Zv2Npu:(LambdaLys-H6) (S352)","v":0,"experiment":"2QdkqwtHKoFxowWwJ","product":"Plasmid"}},"protocol":{"v":4,"name":"Heat shock plasmid transformation (0 -> 1 plasmid)","_id":"mJNyMCoEko3JKRv5x"}},"x/k3dQQxjotyf2D7pi7":{"protocol":{"name":"Pick a colony carrying a plasmid from an agar plate","v":0,"_id":"maDx7F6ry8Mw5JmKf"},"params":{"64ce87c57c5b9a1a536ccd08a47740b3":{"experiment":"acoW4M3QEL98pFbL2","v":0,"type":"experiment","text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord1","product":"Agar plate"}},"products":{"3bbfb159bcd6317e17bc0125824b5a25":{"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+ord1","10990dcf66373fff11ef907bc2b1ef97":"K"}},"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord1","type":{"name":"Bacteria carrying a plasmid","v":2,"_id":"PpBkfrLAqTw2CwNZX"},"allTypes":[{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"}]}},"finishDate":1412200800,"values":{}},"x/HM9Ffidst8AdpRsRM":{"products":{"3bbfb159bcd6317e17bc0125824b5a25":{"properties":{"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)"},"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"K","0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+ord3"}},"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord3","type":{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2},"allTypes":[{"_id":"YW7epnToTmqmHEEwx","name":"Bacteria","v":0},{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2}]}},"finishDate":1412200800,"values":{},"protocol":{"_id":"maDx7F6ry8Mw5JmKf","v":0,"name":"Pick a colony carrying a plasmid from an agar plate"},"params":{"64ce87c57c5b9a1a536ccd08a47740b3":{"product":"Agar plate","type":"experiment","text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord3","v":0,"experiment":"BAL4hfMeRWZKv4kBP"}}},"x/M8gDkacjKYEogMbGK":{"finishDate":1412200800,"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord1","type":{"_id":"EZz9pqmLYbZXhdQuZ","v":3,"name":"Bacterial culture carrying a plasmid"},"allTypes":[{"name":"Bacteria carrying a plasmid","v":2,"_id":"PpBkfrLAqTw2CwNZX"},{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3}],"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"K","0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+ord1"}}}},"values":{"0":{"1":"K","0":""},"1":{"0":"37","1":0}},"protocol":{"name":"Make a liquid culture carrying a plasmid","v":5,"_id":"9TiKPrpdykEiSHFcy"},"params":{"87f8a6ab85c9ced3702b4ea641ad4bb5":{"type":"experiment","text":"Lysogeny broth + ","experiment":"aJkHPGccpLkiga4w5","v":1,"product":"Medium"},"4c0984e0a4d80995f3099e5c33188ce1":{"type":"experiment","text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord1","experiment":"k3dQQxjotyf2D7pi7","v":0,"product":"Colony"}}},"x/PTJLKEEFxQYgTvqiL":{"params":{"87f8a6ab85c9ced3702b4ea641ad4bb5":{"product":"Medium","v":1,"experiment":"aJkHPGccpLkiga4w5","text":"Lysogeny broth + ","type":"experiment"},"4c0984e0a4d80995f3099e5c33188ce1":{"type":"experiment","text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord3","v":0,"experiment":"HM9Ffidst8AdpRsRM","product":"Colony"}},"protocol":{"_id":"9TiKPrpdykEiSHFcy","v":5,"name":"Make a liquid culture carrying a plasmid"},"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+ord3","10990dcf66373fff11ef907bc2b1ef97":"K"},"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)"}},"allTypes":[{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"},{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3}],"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord3","type":{"v":3,"name":"Bacterial culture carrying a plasmid","_id":"EZz9pqmLYbZXhdQuZ"}}},"finishDate":1412200800,"values":{"0":{"1":"K","0":""},"1":{"1":0,"0":"37"}}},"x/uHNMNoyBH4AnMSgxN":{"protocol":{"v":3,"name":"Restriction digest (NEB)","_id":"ENCvtL4jWZQW72hE5"},"params":{"5d37875fd1572e9ebc236d502ab9a20d":[{"paramValue":{"text":"pSBX4C5-2Zv2Npu:LaLys+bad1 (O244)","type":"experiment","experiment":"q285t6PsFQkLk8vgL","v":0,"product":"Purified plasmid"},"values":{"0":{"1":"1.99"}}}],"27dff5d16ea89c5bf5a9db7db12f4ca3":[{"paramValue":{"supply":"mJp39wok46zsmTPmC","v":2,"type":"supply","text":"EcoRI-HF"},"values":{"2":{"1":"1"}}},{"paramValue":{"text":"SpeI-HF","type":"supply","v":2,"supply":"ksAyZFzqCgPhMmBW3"},"values":{"2":{"1":"2"}}}]},"finishDate":1412200800,"products":{"396c983d4290901e4060ffe25e7c0eb3":{"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"pSBX4C5-2Zv2Npu:LaLys+bad1 (E/S)","6f8ac39f837ee4598b7358ce18aec601":null,"4da843835f15245d46602c3d57b579b6":null}},"type":{"_id":"k9LEWTcbmQn53Gzo7","v":3,"name":"dsDNA"},"text":"pSBX4C5-2Zv2Npu:LaLys+bad1 (E/S)","allTypes":[{"name":"dsDNA","v":3,"_id":"k9LEWTcbmQn53Gzo7"}]}},"values":{"0":{"0":"17.51"},"4":{"0":"","1":""},"3":{"0":"","2":"pSBX4C5-2Zv2Npu:LaLys+bad1 (E/S)","1":""},"1":{"1":"2.5","0":"CutSmart"}}},"x/Wjz8FXBaMhpzD6XYa":{"values":{},"products":{"3bbfb159bcd6317e17bc0125824b5a25":{"type":{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2},"text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","allTypes":[{"_id":"YW7epnToTmqmHEEwx","name":"Bacteria","v":0},{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2}],"properties":{"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo"},"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","10990dcf66373fff11ef907bc2b1ef97":"C"}}}},"finishDate":1412253900,"params":{"64ce87c57c5b9a1a536ccd08a47740b3":{"type":"experiment","text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","v":0,"experiment":"YnFuR29JeiymkDfNk","product":"Agar plate"}},"protocol":{"_id":"maDx7F6ry8Mw5JmKf","name":"Pick a colony carrying a plasmid from an agar plate","v":0}},"x/ErB8acFosEtZbCJKX":{"protocol":{"_id":"maDx7F6ry8Mw5JmKf","name":"Pick a colony carrying a plasmid from an agar plate","v":0},"params":{"64ce87c57c5b9a1a536ccd08a47740b3":{"product":"Agar plate","text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)","type":"experiment","v":0,"experiment":"zWzMnbbH8dCgkKnHN"}},"values":{},"products":{"3bbfb159bcd6317e17bc0125824b5a25":{"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"C","0ac71517d2d368cdbabf8b9914716902":"pSB1C3-2Zv2Npu:(LambdaLys-H6)"}},"type":{"name":"Bacteria carrying a plasmid","v":2,"_id":"PpBkfrLAqTw2CwNZX"},"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)","allTypes":[{"name":"Bacteria","v":0,"_id":"YW7epnToTmqmHEEwx"},{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2}]}},"finishDate":1412253900},"x/5nEgNGr7XvKq2QFmF":{"protocol":{"_id":"NgpLzurL2RKFojWWA","name":"PCR from Bacteria (colony PCR or genomic amplification)","v":4},"params":{"dd152b9c02bb725f7af5038f5b1649ac":[{"paramValue":{"v":0,"supply":"usnvt25q5X9ZfqTEL","text":"VF2 (1)","type":"supply"},"values":{"2":{"0":"0.375"}}},{"values":{"2":{"0":"0.375"}},"paramValue":{"type":"supply","text":"VR (2)","v":1,"supply":"JPLm2aiw2BAcSbmcY"}}],"4c0984e0a4d80995f3099e5c33188ce1":{"type":"experiment","text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","experiment":"Wjz8FXBaMhpzD6XYa","v":0,"product":"Colony"}},"products":{"c3aa9e8a1d807a1fafce9630fe0e797b":{"allTypes":[{"v":3,"name":"dsDNA","_id":"k9LEWTcbmQn53Gzo7"}],"text":"Colony PCR: pSB**3-T7RBS-LambdaLys-IGEMHD_v3","type":{"name":"dsDNA","v":3,"_id":"k9LEWTcbmQn53Gzo7"},"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"Colony PCR: pSB**3-T7RBS-LambdaLys-IGEMHD_v3","6f8ac39f837ee4598b7358ce18aec601":null,"4da843835f15245d46602c3d57b579b6":"2"}}}},"finishDate":1412257500,"values":{"3":{"0":"2"},"4":{"3":"30","6":"68","10":300,"0":"94","9":72,"5":"30","1":"120","11":"Colony PCR: pSB**3-T7RBS-LambdaLys-IGEMHD_v3","2":"94","8":"26","4":"52","7":"150"}}},"x/zuJGeAXjqkuj52jEM":{"values":{},"products":{"3bbfb159bcd6317e17bc0125824b5a25":{"text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","type":{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"},"allTypes":[{"v":0,"name":"Bacteria","_id":"YW7epnToTmqmHEEwx"},{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2}],"properties":{"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo"},"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"C","0ac71517d2d368cdbabf8b9914716902":"pSB1C3-T7RBS-LambdaLys-IGEMHD_v3"}}}},"finishDate":1412257500,"params":{"64ce87c57c5b9a1a536ccd08a47740b3":{"text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","type":"experiment","experiment":"YnFuR29JeiymkDfNk","v":0,"product":"Agar plate"}},"protocol":{"_id":"maDx7F6ry8Mw5JmKf","v":0,"name":"Pick a colony carrying a plasmid from an agar plate"}},"x/inoqLKm3FEQdnhxgR":{"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"experiment":"zuJGeAXjqkuj52jEM","v":0,"type":"experiment","text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","product":"Colony"},"dd152b9c02bb725f7af5038f5b1649ac":[{"paramValue":{"v":0,"supply":"usnvt25q5X9ZfqTEL","text":"VF2 (1)","type":"supply"},"values":{"2":{"0":"0.375"}}},{"values":{"2":{"0":"0.375"}},"paramValue":{"type":"supply","text":"VR (2)","supply":"JPLm2aiw2BAcSbmcY","v":1}}]},"protocol":{"v":4,"name":"PCR from Bacteria (colony PCR or genomic amplification)","_id":"NgpLzurL2RKFojWWA"},"finishDate":1412257500,"products":{"c3aa9e8a1d807a1fafce9630fe0e797b":{"allTypes":[{"name":"dsDNA","v":3,"_id":"k9LEWTcbmQn53Gzo7"}],"type":{"_id":"k9LEWTcbmQn53Gzo7","name":"dsDNA","v":3},"text":"Colony PCR: pSB**3-T7RBS-LambdaLys-IGEMHD_v3","properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"Colony PCR: pSB**3-T7RBS-LambdaLys-IGEMHD_v3","4da843835f15245d46602c3d57b579b6":"2","6f8ac39f837ee4598b7358ce18aec601":null}}}},"values":{"3":{"0":"2"},"4":{"0":"94","3":"30","10":300,"6":"68","7":"150","11":"Colony PCR: pSB**3-T7RBS-LambdaLys-IGEMHD_v3","2":"94","9":72,"1":"120","5":"30","4":"52","8":"26"}}},"x/oyPf57gmX7tmv2Sgn":{"protocol":{"name":"Pick a colony carrying a plasmid from an agar plate","v":0,"_id":"maDx7F6ry8Mw5JmKf"},"params":{"64ce87c57c5b9a1a536ccd08a47740b3":{"text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","type":"experiment","experiment":"YnFuR29JeiymkDfNk","v":0,"product":"Agar plate"}},"values":{},"finishDate":1412257500,"products":{"3bbfb159bcd6317e17bc0125824b5a25":{"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"C","0ac71517d2d368cdbabf8b9914716902":"pSB1C3-T7RBS-LambdaLys-IGEMHD_v3"}},"text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","type":{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"},"allTypes":[{"_id":"YW7epnToTmqmHEEwx","name":"Bacteria","v":0},{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2}]}}},"x/fWfKLEbS7nZj9hsuH":{"protocol":{"_id":"NgpLzurL2RKFojWWA","v":4,"name":"PCR from Bacteria (colony PCR or genomic amplification)"},"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"type":"experiment","text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","v":0,"experiment":"oyPf57gmX7tmv2Sgn","product":"Colony"},"dd152b9c02bb725f7af5038f5b1649ac":[{"paramValue":{"supply":"usnvt25q5X9ZfqTEL","v":0,"text":"VF2 (1)","type":"supply"},"values":{"2":{"0":"0.375"}}},{"values":{"2":{"0":"0.375"}},"paramValue":{"v":1,"supply":"JPLm2aiw2BAcSbmcY","type":"supply","text":"VR (2)"}}]},"values":{"3":{"0":"2"},"4":{"4":"52","8":"26","11":"Colony PCR: pSB**3-T7RBS-LambdaLys-IGEMHD_v3","2":"94","9":72,"5":"30","1":"120","7":"150","10":300,"6":"68","3":"30","0":"94"}},"products":{"c3aa9e8a1d807a1fafce9630fe0e797b":{"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"Colony PCR: pSB**3-T7RBS-LambdaLys-IGEMHD_v3","4da843835f15245d46602c3d57b579b6":"2","6f8ac39f837ee4598b7358ce18aec601":null}},"type":{"_id":"k9LEWTcbmQn53Gzo7","name":"dsDNA","v":3},"text":"Colony PCR: pSB**3-T7RBS-LambdaLys-IGEMHD_v3","allTypes":[{"v":3,"name":"dsDNA","_id":"k9LEWTcbmQn53Gzo7"}]}},"finishDate":1412257500},"x/xZobtSDRX3kTXL7mM":{"protocol":{"v":4,"name":"PCR from Bacteria (colony PCR or genomic amplification)","_id":"NgpLzurL2RKFojWWA"},"params":{"dd152b9c02bb725f7af5038f5b1649ac":[{"values":{"2":{"0":"0.375"}},"paramValue":{"type":"supply","text":"VF2 (1)","supply":"usnvt25q5X9ZfqTEL","v":0}},{"values":{"2":{"0":"0.375"}},"paramValue":{"type":"supply","text":"VR (2)","v":1,"supply":"JPLm2aiw2BAcSbmcY"}}],"4c0984e0a4d80995f3099e5c33188ce1":{"experiment":"ErB8acFosEtZbCJKX","v":0,"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)","type":"experiment","product":"Colony"}},"finishDate":1412257500,"products":{"c3aa9e8a1d807a1fafce9630fe0e797b":{"properties":{"k9LEWTcbmQn53Gzo7":{"6f8ac39f837ee4598b7358ce18aec601":null,"4da843835f15245d46602c3d57b579b6":"2","49ee3087348e8d44e1feda1917443987":"Colony PCR: pSB**3-2Zv2Npu:(LambdaLys-H6)"}},"type":{"v":3,"name":"dsDNA","_id":"k9LEWTcbmQn53Gzo7"},"text":"Colony PCR: pSB**3-2Zv2Npu:(LambdaLys-H6)","allTypes":[{"v":3,"name":"dsDNA","_id":"k9LEWTcbmQn53Gzo7"}]}},"values":{"4":{"0":"94","3":"30","10":300,"6":"68","7":"150","11":"Colony PCR: pSB**3-2Zv2Npu:(LambdaLys-H6)","2":"94","5":"30","9":72,"1":"120","4":"52","8":"26"},"3":{"0":"2"}}},"x/poCbsebcueW9W6TaD":{"finishDate":1412257500,"products":{"3bbfb159bcd6317e17bc0125824b5a25":{"properties":{"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo"},"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSB1C3-2Zv2Npu:(LambdaLys-H6)","10990dcf66373fff11ef907bc2b1ef97":"C"}},"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)","type":{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"},"allTypes":[{"_id":"YW7epnToTmqmHEEwx","name":"Bacteria","v":0},{"name":"Bacteria carrying a plasmid","v":2,"_id":"PpBkfrLAqTw2CwNZX"}]}},"values":{},"params":{"64ce87c57c5b9a1a536ccd08a47740b3":{"product":"Agar plate","experiment":"zWzMnbbH8dCgkKnHN","v":0,"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)","type":"experiment"}},"protocol":{"_id":"maDx7F6ry8Mw5JmKf","v":0,"name":"Pick a colony carrying a plasmid from an agar plate"}},"x/Sk583SAPXEtaM7Pe9":{"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"type":"experiment","text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)","v":0,"experiment":"poCbsebcueW9W6TaD","product":"Colony"},"dd152b9c02bb725f7af5038f5b1649ac":[{"values":{"2":{"0":"0.375"}},"paramValue":{"text":"VF2 (1)","type":"supply","supply":"usnvt25q5X9ZfqTEL","v":0}},{"values":{"2":{"0":"0.375"}},"paramValue":{"v":1,"supply":"JPLm2aiw2BAcSbmcY","type":"supply","text":"VR (2)"}}]},"protocol":{"name":"PCR from Bacteria (colony PCR or genomic amplification)","v":4,"_id":"NgpLzurL2RKFojWWA"},"values":{"4":{"7":"150","4":"52","8":"26","11":"Colony PCR: pSB**3-2Zv2Npu:(LambdaLys-H6)","2":"94","1":"120","9":72,"5":"30","0":"94","10":300,"6":"68","3":"30"},"3":{"0":"2"}},"finishDate":1412257500,"products":{"c3aa9e8a1d807a1fafce9630fe0e797b":{"text":"Colony PCR: pSB**3-2Zv2Npu:(LambdaLys-H6)","type":{"name":"dsDNA","v":3,"_id":"k9LEWTcbmQn53Gzo7"},"allTypes":[{"name":"dsDNA","v":3,"_id":"k9LEWTcbmQn53Gzo7"}],"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"Colony PCR: pSB**3-2Zv2Npu:(LambdaLys-H6)","6f8ac39f837ee4598b7358ce18aec601":null,"4da843835f15245d46602c3d57b579b6":"2"}}}}},"x/tqtX2sotBCZ9byTrc":{"values":{},"products":{"3bbfb159bcd6317e17bc0125824b5a25":{"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSB1C3-2Zv2Npu:(LambdaLys-H6)","10990dcf66373fff11ef907bc2b1ef97":"C"}},"allTypes":[{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2}],"type":{"name":"Bacteria carrying a plasmid","v":2,"_id":"PpBkfrLAqTw2CwNZX"},"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)"}},"finishDate":1412257500,"params":{"64ce87c57c5b9a1a536ccd08a47740b3":{"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)","type":"experiment","v":0,"experiment":"zWzMnbbH8dCgkKnHN","product":"Agar plate"}},"protocol":{"_id":"maDx7F6ry8Mw5JmKf","name":"Pick a colony carrying a plasmid from an agar plate","v":0}},"x/sC5FdnAgvA72NhKEi":{"finishDate":1412257500,"products":{"c3aa9e8a1d807a1fafce9630fe0e797b":{"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"Colony PCR: pSB**3-2Zv2Npu:(LambdaLys-H6)","4da843835f15245d46602c3d57b579b6":"2","6f8ac39f837ee4598b7358ce18aec601":null}},"text":"Colony PCR: pSB**3-2Zv2Npu:(LambdaLys-H6)","type":{"_id":"k9LEWTcbmQn53Gzo7","v":3,"name":"dsDNA"},"allTypes":[{"v":3,"name":"dsDNA","_id":"k9LEWTcbmQn53Gzo7"}]}},"values":{"4":{"7":"150","9":72,"1":"120","5":"30","2":"94","11":"Colony PCR: pSB**3-2Zv2Npu:(LambdaLys-H6)","8":"26","4":"52","0":"94","3":"30","6":"68","10":300},"3":{"0":"2"}},"protocol":{"_id":"NgpLzurL2RKFojWWA","name":"PCR from Bacteria (colony PCR or genomic amplification)","v":4},"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"product":"Colony","v":0,"experiment":"tqtX2sotBCZ9byTrc","text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)","type":"experiment"},"dd152b9c02bb725f7af5038f5b1649ac":[{"values":{"2":{"0":"0.375"}},"paramValue":{"type":"supply","text":"VF2 (1)","v":0,"supply":"usnvt25q5X9ZfqTEL"}},{"paramValue":{"type":"supply","text":"VR (2)","supply":"JPLm2aiw2BAcSbmcY","v":1},"values":{"2":{"0":"0.375"}}}]}},"x/4uoGbkwK4ThghexZc":{"products":{"12327177bfe077ccd10daa3d5ca9809c":{"properties":{},"allTypes":[{"_id":"QCyBocmukGKrzbkYb","name":"Agarose Gel","v":0}],"text":"","type":{"_id":"QCyBocmukGKrzbkYb","v":0,"name":"Agarose Gel"}}},"finishDate":1412267400,"sid":"423","values":{"11":{"1":"","0":""}},"params":{"5d37875fd1572e9ebc236d502ab9a20d":[{"paramValue":{"v":0,"supply":"PtEdhNN8Q7dYgDbtw","type":"supply","text":"2-log DNA ladder (NEB)"},"values":{"10":{"0":"3.0"}}},{"paramValue":{"text":"Colony PCR: pSB**3-2Zv2Npu","type":"experiment","experiment":"wgXbEcFM9tpixaYNG","v":0,"product":"Amplificate"},"values":{"10":{"0":""}}},{"values":{"10":{"0":""}},"paramValue":{"v":0,"experiment":"ckuWjf4fd5geuAM2Y","type":"experiment","text":"Colony PCR: pSB**3-2Zv2Npu","product":"Amplificate"}},{"paramValue":{"v":0,"experiment":"i7ubvk2BtTpEeqe6E","text":"Colony PCR: pSB**3-2Zv2Npu","type":"experiment","product":"Amplificate"},"values":{"10":{"0":""}}},{"paramValue":{"experiment":"5nEgNGr7XvKq2QFmF","v":0,"text":"Colony PCR: pSB**3-T7RBS-LambdaLys-IGEMHD_v3","type":"experiment","product":"Amplificate"},"values":{"10":{"0":""}}},{"values":{"10":{"0":""}},"paramValue":{"product":"Amplificate","type":"experiment","text":"Colony PCR: pSB**3-T7RBS-LambdaLys-IGEMHD_v3","experiment":"inoqLKm3FEQdnhxgR","v":0}},{"paramValue":{"experiment":"fWfKLEbS7nZj9hsuH","v":0,"type":"experiment","text":"Colony PCR: pSB**3-T7RBS-LambdaLys-IGEMHD_v3","product":"Amplificate"},"values":{"10":{"0":""}}},{"values":{"10":{"0":""}},"paramValue":{"product":"Amplificate","experiment":"FGXHrWDCkvspb8fuf","v":0,"text":"Colony PCR: pSB**3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6","type":"experiment"}},{"paramValue":{"experiment":"LkSzbaEayhTEwGDv5","v":0,"text":"Colony PCR: pSB**3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6","type":"experiment","product":"Amplificate"},"values":{"10":{"0":""}}},{"paramValue":{"product":"Amplificate","v":0,"experiment":"LtBxKPFZq5N6CLGzw","type":"experiment","text":"Colony PCR: pSB**3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6"},"values":{"10":{"0":""}}},{"paramValue":{"type":"experiment","text":"Colony PCR: pSB**3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6","experiment":"6aG3c5AKRmi6cBPq5","v":0,"product":"Amplificate"},"values":{"10":{"0":""}}},{"values":{"10":{"0":""}},"paramValue":{"type":"experiment","text":"Colony PCR: pSB**3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6","v":0,"experiment":"AmYQT4iRPfgRN4QZf","product":"Amplificate"}},{"values":{"10":{"0":""}},"paramValue":{"experiment":"mwhf3ZmfR4GDswnkB","v":0,"type":"experiment","text":"Colony PCR: pSB**3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6","product":"Amplificate"}},{"values":{"10":{"0":""}},"paramValue":{"v":0,"experiment":"Hs9nozJouhxNigaqB","type":"experiment","text":"Colony PCR: pSB**3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6","product":"Amplificate"}},{"paramValue":{"text":"Colony PCR: pSB**3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6","type":"experiment","v":0,"experiment":"uv5bFmJAzj8PFymz6","product":"Amplificate"},"values":{"10":{"0":""}}},{"paramValue":{"product":"Amplificate","experiment":"eTKLtz9YY3cvwzqH7","v":0,"type":"experiment","text":"Colony PCR: pSB**3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6"},"values":{"10":{"0":""}}},{"values":{"10":{"0":""}},"paramValue":{"product":"Amplificate","experiment":"xZobtSDRX3kTXL7mM","v":0,"text":"Colony PCR: pSB**3-2Zv2Npu:(LambdaLys-H6)","type":"experiment"}},{"values":{"10":{"0":""}},"paramValue":{"experiment":"Sk583SAPXEtaM7Pe9","v":0,"type":"experiment","text":"Colony PCR: pSB**3-2Zv2Npu:(LambdaLys-H6)","product":"Amplificate"}},{"values":{"10":{"0":""}},"paramValue":{"text":"Colony PCR: pSB**3-2Zv2Npu:(LambdaLys-H6)","type":"experiment","v":0,"experiment":"sC5FdnAgvA72NhKEi","product":"Amplificate"}},{"paramValue":{"text":"2-log DNA ladder (NEB)","type":"supply","supply":"PtEdhNN8Q7dYgDbtw","v":0},"values":{"10":{"0":""}}}]},"protocol":{"name":"Gel Electrophoresis","v":1,"_id":"JPmjNykwWASQiD9PX"}},"x/Lc5EhnbXEsryy6Bu8":{"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"product":"Colony","text":"E. coli Turbo with pSB1C3-LiLaLy(H6)","type":"experiment","v":0,"experiment":"cfo3NdtsBPEQaDZWh"},"87f8a6ab85c9ced3702b4ea641ad4bb5":{"product":"Medium","type":"experiment","text":"Lysogeny broth + ","v":1,"experiment":"aJkHPGccpLkiga4w5"}},"protocol":{"v":5,"name":"Make a liquid culture carrying a plasmid","_id":"9TiKPrpdykEiSHFcy"},"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"text":"E. coli Turbo with pSB1C3-LiLaLy(H6)","type":{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3},"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","v":2,"name":"Bacteria carrying a plasmid"},{"name":"Bacteria","v":0,"_id":"YW7epnToTmqmHEEwx"},{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3}],"properties":{"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSB1C3-LiLaLy(H6)","10990dcf66373fff11ef907bc2b1ef97":"C"},"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e1520b5997a532c7889f6e8883920ab8":"E. coli"}}}},"finishDate":1412269200,"values":{"1":{"0":"37","1":0},"0":{"0":"","1":"C"}}},"x/J3MCuPyorqYMnRXxq":{"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"C","0ac71517d2d368cdbabf8b9914716902":"pSB1C3-T7RBS-LambdaLys-IGEMHD_v3"},"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo"}},"type":{"_id":"EZz9pqmLYbZXhdQuZ","v":3,"name":"Bacterial culture carrying a plasmid"},"text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2},{"v":0,"name":"Bacteria","_id":"YW7epnToTmqmHEEwx"},{"name":"Bacterial culture carrying a plasmid","v":3,"_id":"EZz9pqmLYbZXhdQuZ"}]}},"finishDate":1412269200,"values":{"1":{"0":"37","1":0},"0":{"1":"C","0":""}},"params":{"87f8a6ab85c9ced3702b4ea641ad4bb5":{"product":"Medium","type":"experiment","text":"Lysogeny broth + ","v":1,"experiment":"aJkHPGccpLkiga4w5"},"4c0984e0a4d80995f3099e5c33188ce1":{"product":"Colony","text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","type":"experiment","experiment":"Wjz8FXBaMhpzD6XYa","v":0}},"protocol":{"name":"Make a liquid culture carrying a plasmid","v":5,"_id":"9TiKPrpdykEiSHFcy"}},"x/fS8pevAjX5zzW3PpP":{"params":{"87f8a6ab85c9ced3702b4ea641ad4bb5":{"text":"Lysogeny broth + ","type":"experiment","v":1,"experiment":"aJkHPGccpLkiga4w5","product":"Medium"},"4c0984e0a4d80995f3099e5c33188ce1":{"type":"experiment","text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)","experiment":"ErB8acFosEtZbCJKX","v":0,"product":"Colony"}},"protocol":{"v":5,"name":"Make a liquid culture carrying a plasmid","_id":"9TiKPrpdykEiSHFcy"},"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2},{"v":0,"name":"Bacteria","_id":"YW7epnToTmqmHEEwx"},{"v":3,"name":"Bacterial culture carrying a plasmid","_id":"EZz9pqmLYbZXhdQuZ"}],"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)","type":{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3},"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"C","0ac71517d2d368cdbabf8b9914716902":"pSB1C3-2Zv2Npu:(LambdaLys-H6)"}}}},"finishDate":1412269200,"values":{"1":{"1":0,"0":"37"},"0":{"1":"C","0":""}}},"x/nZTeKbnhiFz65uXuz":{"protocol":{"_id":"9TiKPrpdykEiSHFcy","v":5,"name":"Make a liquid culture carrying a plasmid"},"params":{"87f8a6ab85c9ced3702b4ea641ad4bb5":{"product":"Medium","experiment":"aJkHPGccpLkiga4w5","v":1,"text":"Lysogeny broth + ","type":"experiment"},"4c0984e0a4d80995f3099e5c33188ce1":{"product":"Colony","experiment":"W65ELDqk8Zpr2zjNz","v":0,"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-Rigid)","type":"experiment"}},"values":{"0":{"1":"C","0":""},"1":{"1":0,"0":"37"}},"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"C","0ac71517d2d368cdbabf8b9914716902":"pSB1C3-2Zv2Npu:(LambdaLys-Rigid)"},"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e1520b5997a532c7889f6e8883920ab8":"E. coli"}},"type":{"v":3,"name":"Bacterial culture carrying a plasmid","_id":"EZz9pqmLYbZXhdQuZ"},"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-Rigid)","allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","v":2,"name":"Bacteria carrying a plasmid"},{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3}]}},"finishDate":1412269200},"x/9gEMQYTPv9wsHp4Pp":{"protocol":{"_id":"9TiKPrpdykEiSHFcy","v":5,"name":"Make a liquid culture carrying a plasmid"},"params":{"87f8a6ab85c9ced3702b4ea641ad4bb5":{"product":"Medium","v":1,"experiment":"aJkHPGccpLkiga4w5","text":"Lysogeny broth + ","type":"experiment"},"4c0984e0a4d80995f3099e5c33188ce1":{"product":"Colony","experiment":"a3fFftNeSis3TjbX7","v":0,"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord1","type":"experiment"}},"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+ord1","10990dcf66373fff11ef907bc2b1ef97":"K"}},"allTypes":[{"name":"Bacteria carrying a plasmid","v":2,"_id":"PpBkfrLAqTw2CwNZX"},{"_id":"YW7epnToTmqmHEEwx","name":"Bacteria","v":0},{"_id":"EZz9pqmLYbZXhdQuZ","v":3,"name":"Bacterial culture carrying a plasmid"}],"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord1","type":{"_id":"EZz9pqmLYbZXhdQuZ","v":3,"name":"Bacterial culture carrying a plasmid"}}},"finishDate":1412269200,"values":{"0":{"1":"K","0":""},"1":{"0":"37","1":0}}},"x/FosoDcFEoNkh63Yvx":{"protocol":{"_id":"9TiKPrpdykEiSHFcy","name":"Make a liquid culture carrying a plasmid","v":5},"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"product":"Colony","v":0,"experiment":"kha8Y3drcGjFoTED4","text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord3","type":"experiment"},"87f8a6ab85c9ced3702b4ea641ad4bb5":{"text":"Lysogeny broth + ","type":"experiment","v":1,"experiment":"aJkHPGccpLkiga4w5","product":"Medium"}},"finishDate":1412269200,"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"K","0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+ord3"},"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)","e1520b5997a532c7889f6e8883920ab8":"E. coli"}},"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+ord3","type":{"_id":"EZz9pqmLYbZXhdQuZ","v":3,"name":"Bacterial culture carrying a plasmid"},"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2},{"v":0,"name":"Bacteria","_id":"YW7epnToTmqmHEEwx"},{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3}]}},"values":{"1":{"0":"37","1":0},"0":{"0":"","1":"K"}}},"x/eAzfv8dbS9ofJpJGg":{"protocol":{"name":"Make a liquid culture carrying a plasmid","v":5,"_id":"9TiKPrpdykEiSHFcy"},"params":{"87f8a6ab85c9ced3702b4ea641ad4bb5":{"product":"Medium","text":"Lysogeny broth + ","type":"experiment","experiment":"aJkHPGccpLkiga4w5","v":1},"4c0984e0a4d80995f3099e5c33188ce1":{"text":"E. coli BL21(DE3) with pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3","type":"experiment","experiment":"KCeR3NwfmpW74HoSj","v":0,"product":"Glycerol stock"}},"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2},{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"EZz9pqmLYbZXhdQuZ","v":3,"name":"Bacterial culture carrying a plasmid"}],"text":"E. coli BL21(DE3) with pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3","type":{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3},"properties":{"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3","10990dcf66373fff11ef907bc2b1ef97":"K"},"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)","e1520b5997a532c7889f6e8883920ab8":"E. coli"}}}},"finishDate":1412286300,"values":{"1":{"1":0,"0":"37"},"0":{"1":"K","0":""}}},"x/TaRg9CH4wQqZxvDxG":{"protocol":{"v":5,"name":"Make a liquid culture carrying a plasmid","_id":"9TiKPrpdykEiSHFcy"},"params":{"87f8a6ab85c9ced3702b4ea641ad4bb5":{"experiment":"aJkHPGccpLkiga4w5","v":1,"type":"experiment","text":"Lysogeny broth + ","product":"Medium"},"4c0984e0a4d80995f3099e5c33188ce1":{"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+flexlinker","type":"experiment","experiment":"S3jcXxDzAAxzWjJ8F","v":0,"product":"Glycerol stock"}},"values":{"0":{"0":"","1":"K"},"1":{"1":0,"0":"37"}},"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+flexlinker","10990dcf66373fff11ef907bc2b1ef97":"K"}},"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","v":2,"name":"Bacteria carrying a plasmid"},{"name":"Bacteria","v":0,"_id":"YW7epnToTmqmHEEwx"},{"name":"Bacterial culture carrying a plasmid","v":3,"_id":"EZz9pqmLYbZXhdQuZ"}],"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+flexlinker","type":{"_id":"EZz9pqmLYbZXhdQuZ","v":3,"name":"Bacterial culture carrying a plasmid"}}},"finishDate":1412286300},"x/wPaH4t9FHoPyfBGg8":{"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"product":"Glycerol stock","type":"experiment","text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+may1","v":0,"experiment":"oRLoLYGWwMFm84ebL"},"87f8a6ab85c9ced3702b4ea641ad4bb5":{"v":1,"experiment":"aJkHPGccpLkiga4w5","text":"Lysogeny broth + ","type":"experiment","product":"Medium"}},"protocol":{"v":5,"name":"Make a liquid culture carrying a plasmid","_id":"9TiKPrpdykEiSHFcy"},"values":{"1":{"1":0,"0":"37"},"0":{"0":"","1":"K"}},"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"K","0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+may1"},"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)"}},"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+may1","type":{"_id":"EZz9pqmLYbZXhdQuZ","v":3,"name":"Bacterial culture carrying a plasmid"},"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","v":2,"name":"Bacteria carrying a plasmid"},{"v":0,"name":"Bacteria","_id":"YW7epnToTmqmHEEwx"},{"name":"Bacterial culture carrying a plasmid","v":3,"_id":"EZz9pqmLYbZXhdQuZ"}]}},"finishDate":1412286300},"x/b6WaoF67LuhutQ85C":{"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"product":"Glycerol stock","experiment":"YnPT5MFPE7SXPg5sA","v":0,"type":"experiment","text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+sgt2"},"87f8a6ab85c9ced3702b4ea641ad4bb5":{"text":"Lysogeny broth + ","type":"experiment","v":1,"experiment":"aJkHPGccpLkiga4w5","product":"Medium"}},"protocol":{"name":"Make a liquid culture carrying a plasmid","v":5,"_id":"9TiKPrpdykEiSHFcy"},"finishDate":1412286300,"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+sgt2","type":{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3},"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","v":2,"name":"Bacteria carrying a plasmid"},{"name":"Bacteria","v":0,"_id":"YW7epnToTmqmHEEwx"},{"name":"Bacterial culture carrying a plasmid","v":3,"_id":"EZz9pqmLYbZXhdQuZ"}],"properties":{"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"K","0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+sgt2"},"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)"}}}},"values":{"0":{"1":"K","0":""},"1":{"0":"37","1":0}}},"x/JGE8aNnjX55NJxRN6":{"values":{"0":{"1":"K","0":""},"1":{"1":0,"0":"37"}},"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+sho1","10990dcf66373fff11ef907bc2b1ef97":"K"}},"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+sho1","type":{"name":"Bacterial culture carrying a plasmid","v":3,"_id":"EZz9pqmLYbZXhdQuZ"},"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","v":2,"name":"Bacteria carrying a plasmid"},{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"v":3,"name":"Bacterial culture carrying a plasmid","_id":"EZz9pqmLYbZXhdQuZ"}]}},"finishDate":1412286300,"params":{"87f8a6ab85c9ced3702b4ea641ad4bb5":{"product":"Medium","v":1,"experiment":"aJkHPGccpLkiga4w5","text":"Lysogeny broth + ","type":"experiment"},"4c0984e0a4d80995f3099e5c33188ce1":{"product":"Glycerol stock","text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+sho1","type":"experiment","experiment":"XLZkfeRqzZroqei37","v":0}},"protocol":{"v":5,"name":"Make a liquid culture carrying a plasmid","_id":"9TiKPrpdykEiSHFcy"}},"x/biarCERWMy5XTebXJ":{"finishDate":1412286300,"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)"},"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"K","0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+sho2"}},"allTypes":[{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"},{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3}],"text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+sho2","type":{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3}}},"values":{"1":{"1":0,"0":"37"},"0":{"1":"K","0":""}},"params":{"87f8a6ab85c9ced3702b4ea641ad4bb5":{"experiment":"aJkHPGccpLkiga4w5","v":1,"type":"experiment","text":"Lysogeny broth + ","product":"Medium"},"4c0984e0a4d80995f3099e5c33188ce1":{"v":0,"experiment":"cpxFbJ8EbmkyfD7du","type":"experiment","text":"E. coli BL21(DE3) with pSBX1K3-2Zv2Npu:LaLys+sho2","product":"Glycerol stock"}},"protocol":{"name":"Make a liquid culture carrying a plasmid","v":5,"_id":"9TiKPrpdykEiSHFcy"}},"x/T42tRFDJ2W5hctozc":{"values":{},"products":{"48ee462e132458ff1db490fd0fe20e0a":{"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"C","0ac71517d2d368cdbabf8b9914716902":"pSB1C3-T7RBS-LambdaLys-IGEMHD_v3"}},"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2},{"name":"Bacteria","v":0,"_id":"YW7epnToTmqmHEEwx"},{"name":"Glycerol stock of bacteria carrying a plasmid","v":2,"_id":"aGtyYHkAAF7Qcrhic"}],"text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","type":{"_id":"aGtyYHkAAF7Qcrhic","v":2,"name":"Glycerol stock of bacteria carrying a plasmid"}}},"finishDate":1412287200,"params":{"6926f2fd88f899a22a561f0e808aa8a9":{"product":"Culture","type":"experiment","text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","experiment":"J3MCuPyorqYMnRXxq","v":0}},"protocol":{"_id":"E8wraGbztRq8EsET7","v":1,"name":"Make a glycerol stock of bacteria carrying a plasmid"}},"x/5TgzJvHDH8fueeQ32":{"params":{"6926f2fd88f899a22a561f0e808aa8a9":{"type":"experiment","text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)","v":0,"experiment":"fS8pevAjX5zzW3PpP","product":"Culture"}},"protocol":{"name":"Make a glycerol stock of bacteria carrying a plasmid","v":1,"_id":"E8wraGbztRq8EsET7"},"values":{},"finishDate":1412287200,"products":{"48ee462e132458ff1db490fd0fe20e0a":{"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2},{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"aGtyYHkAAF7Qcrhic","name":"Glycerol stock of bacteria carrying a plasmid","v":2}],"type":{"_id":"aGtyYHkAAF7Qcrhic","name":"Glycerol stock of bacteria carrying a plasmid","v":2},"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)","properties":{"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"C","0ac71517d2d368cdbabf8b9914716902":"pSB1C3-2Zv2Npu:(LambdaLys-H6)"},"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo"}}}}},"x/F9TsKQXGLqgNgvFyj":{"protocol":{"name":"Make a glycerol stock of bacteria carrying a plasmid","v":1,"_id":"E8wraGbztRq8EsET7"},"params":{"6926f2fd88f899a22a561f0e808aa8a9":{"product":"Culture","text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-Rigid)","type":"experiment","v":0,"experiment":"nZTeKbnhiFz65uXuz"}},"finishDate":1412287200,"products":{"48ee462e132458ff1db490fd0fe20e0a":{"text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-Rigid)","type":{"_id":"aGtyYHkAAF7Qcrhic","name":"Glycerol stock of bacteria carrying a plasmid","v":2},"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2},{"name":"Bacteria","v":0,"_id":"YW7epnToTmqmHEEwx"},{"name":"Glycerol stock of bacteria carrying a plasmid","v":2,"_id":"aGtyYHkAAF7Qcrhic"}],"properties":{"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo"},"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSB1C3-2Zv2Npu:(LambdaLys-Rigid)","10990dcf66373fff11ef907bc2b1ef97":"C"}}}},"values":{}},"x/FMQakc7eX2yZhpimC":{"protocol":{"name":"Plasmid ligation with T4 DNA Ligase (NEB)","v":1,"_id":"n4Sg8s8AfhMEtQtFH"},"params":{"5d37875fd1572e9ebc236d502ab9a20d":[{"values":{"1":{"0":"5.681"}},"paramValue":{"text":"pSBX1K3-BBa_I13507 (E/S)","type":"experiment","experiment":"dcKkqcvgRcxX3scST","v":0,"product":"Excised DNA fragment"}},{"paramValue":{"product":"Excised DNA fragment","type":"experiment","text":"pSBX4C5-2Zv2Npu:LaLys+bad1 (E/S)","v":0,"experiment":"zr8nwCssJQoDuS7XA"},"values":{"1":{"0":"7.069"}}}]},"values":{"1":{"1":"pSBX1K3-2Zv2Npu:LaLys+bad1","3":"K","2":"S284","4":"11.34464517"},"0":{"1":"1.5","0":"0"}},"finishDate":1412287200,"products":{"63aa3271e10f79baaba8465140e427e9":{"allTypes":[{"name":"dsDNA","v":3,"_id":"k9LEWTcbmQn53Gzo7"},{"_id":"cvt43tybpDTWdk2xR","name":"Plasmid","v":4}],"type":{"v":4,"name":"Plasmid","_id":"cvt43tybpDTWdk2xR"},"text":"pSBX1K3-2Zv2Npu:LaLys+bad1 (S284)","properties":{"cvt43tybpDTWdk2xR":{"c29e15397d29fc94dad70016db7ab2e9":"K"},"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"pSBX1K3-2Zv2Npu:LaLys+bad1","6f8ac39f837ee4598b7358ce18aec601":"S284","4da843835f15245d46602c3d57b579b6":"11.34464517"}}}}},"x/Nyz6PoeHC5sEdR9Lo":{"params":{"6926f2fd88f899a22a561f0e808aa8a9":{"product":"Culture","type":"experiment","text":"E. coli Turbo with pSB1C3-LiLaLy(H6)","v":0,"experiment":"Lc5EhnbXEsryy6Bu8"}},"protocol":{"_id":"E8wraGbztRq8EsET7","v":1,"name":"Make a glycerol stock of bacteria carrying a plasmid"},"products":{"48ee462e132458ff1db490fd0fe20e0a":{"type":{"v":2,"name":"Glycerol stock of bacteria carrying a plasmid","_id":"aGtyYHkAAF7Qcrhic"},"text":"E. coli Turbo with pSB1C3-LiLaLy(H6)","allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","v":2,"name":"Bacteria carrying a plasmid"},{"name":"Bacteria","v":0,"_id":"YW7epnToTmqmHEEwx"},{"v":2,"name":"Glycerol stock of bacteria carrying a plasmid","_id":"aGtyYHkAAF7Qcrhic"}],"properties":{"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo"},"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"C","0ac71517d2d368cdbabf8b9914716902":"pSB1C3-LiLaLy(H6)"}}}},"finishDate":1412287200,"values":{}},"x/EoZtBq7MsFSj426z6":{"params":{"5d37875fd1572e9ebc236d502ab9a20d":[{"paramValue":{"text":"2-log DNA ladder (NEB)","type":"supply","v":0,"supply":"PtEdhNN8Q7dYgDbtw"},"values":{"10":{"0":"3.0"}}},{"paramValue":{"v":0,"experiment":"p8c6ikrbnoFsAu5C9","type":"experiment","text":"pSB1C3-BBa_J04450 (E/S)","product":"DNA"},"values":{"10":{"0":""}}},{"paramValue":{"product":"DNA","experiment":"L6nMRfbEcsCk3QrQj","v":0,"type":"experiment","text":"pSBX1K3-BBa_I13507 (E/S)"},"values":{"10":{"0":""}}},{"values":{"10":{"0":""}},"paramValue":{"product":"DNA","text":"pSBX4C5-2Zv2Npu:LaLys+bad1 (E/S)","type":"experiment","v":0,"experiment":"uHNMNoyBH4AnMSgxN"}},{"paramValue":{"product":"DNA","text":"pSBX4C5-1GateNpuDnaE(N)*-H6 (E/P)","type":"experiment","v":0,"experiment":"vpayWSrrsHy7C9TrB"},"values":{"10":{"0":""}}},{"values":{"10":{"0":""}},"paramValue":{"product":"DNA","text":"pSBX4C5-1GateSspGyrB-S11C_H6 (E/P)","type":"experiment","v":0,"experiment":"3fghzJSv8YKPkySX8"}},{"values":{"10":{"0":""}},"paramValue":{"product":"DNA","experiment":"6ZTd6B4Kr7YR5Luk5","v":0,"type":"experiment","text":"pSBX4C5-H6-1GateNpu*(C)_v2 (E/P)"}},{"paramValue":{"supply":"PtEdhNN8Q7dYgDbtw","v":0,"type":"supply","text":"2-log DNA ladder (NEB)"},"values":{"10":{"0":""}}}]},"protocol":{"_id":"JPmjNykwWASQiD9PX","name":"Gel Electrophoresis","v":1},"values":{"11":{"0":"","1":""}},"sid":"426","products":{"12327177bfe077ccd10daa3d5ca9809c":{"allTypes":[{"name":"Agarose Gel","v":0,"_id":"QCyBocmukGKrzbkYb"}],"type":{"_id":"QCyBocmukGKrzbkYb","name":"Agarose Gel","v":0},"text":"","properties":{}}},"finishDate":1412361900},"x/Ljq6u8dsJCLC33QS6":{"protocol":{"_id":"kxBySpW3SnbWuYMRw","v":0,"name":"Streak bacteria carrying a plasmid onto an agar plate"},"params":{"a1610acb29b294b95abcdeb4b9fdd74b":{"product":"Bacteria carrying the plasmid","experiment":"M74N2H2cnBTDTEgSq","v":0,"text":"E. coli Turbo with pSBX1K3-2Zv2Npu:LaLys+bad1","type":"experiment"}},"products":{"fccc3bbdd62f7ea544bf80e802c28b89":{"properties":{"D2DD2v2FYpYQLFwji":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+bad1","1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e1520b5997a532c7889f6e8883920ab8":"E. coli","e4963ca4d4576f5cb6f8af52e3d038b6":"K"}},"type":{"_id":"D2DD2v2FYpYQLFwji","v":0,"name":"Agar plate with bacteria carrying a plasmid"},"text":"E. coli Turbo with pSBX1K3-2Zv2Npu:LaLys+bad1","allTypes":[{"_id":"D2DD2v2FYpYQLFwji","v":0,"name":"Agar plate with bacteria carrying a plasmid"}]}},"finishDate":1412377200,"values":{"0":{"1":"K","0":""}}},"x/M74N2H2cnBTDTEgSq":{"finishDate":1412377200,"products":{"1f90a3d695361444cb4d5bd0bc04c741":{"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+bad1","10990dcf66373fff11ef907bc2b1ef97":"K"}},"text":"E. coli Turbo with pSBX1K3-2Zv2Npu:LaLys+bad1","type":{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"},"allTypes":[{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"}]}},"values":{"0":{"0":""},"6":{"0":"","1":"","2":37},"8":{"0":""},"4":{"0":""},"5":{"0":""},"3":{"0":""},"2":{"0":""}},"params":{"63aa3271e10f79baaba8465140e427e9":{"type":"experiment","text":"pSBX1K3-2Zv2Npu:LaLys+bad1 (S284)","v":0,"experiment":"FMQakc7eX2yZhpimC","product":"Plasmid"},"691586db4be0d1aff3ffb8b43b31cc9a":{"type":"experiment","text":"E. coli Turbo","v":0,"experiment":"DWbyZ2rdvbmNS7PKa","product":"Competent cells"}},"protocol":{"name":"Heat shock plasmid transformation (0 -> 1 plasmid)","v":4,"_id":"mJNyMCoEko3JKRv5x"}},"x/pG7d8A8gJmsaQcMK9":{"products":{"a328788326c9ffc983432235a9111315":{"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","4da843835f15245d46602c3d57b579b6":"163","6f8ac39f837ee4598b7358ce18aec601":"O348"},"cvt43tybpDTWdk2xR":{"c29e15397d29fc94dad70016db7ab2e9":"C"}},"allTypes":[{"name":"dsDNA","v":3,"_id":"k9LEWTcbmQn53Gzo7"},{"v":4,"name":"Plasmid","_id":"cvt43tybpDTWdk2xR"}],"type":{"_id":"cvt43tybpDTWdk2xR","name":"Plasmid","v":4},"text":"pSB1C3-T7RBS-LambdaLys-IGEMHD_v3 (O348)"}},"finishDate":1412380800,"values":{"0":{"0":"O348"},"1":{"0":"163"}},"protocol":{"name":"Miniprep","v":2,"_id":"Nm75fDvHCr38nXJBo"},"params":{"1377cefc182c1de1378c2dbd6e230a20":{"text":"E. coli Turbo with pSB1C3-T7RBS-LambdaLys-IGEMHD_v3","type":"experiment","v":0,"experiment":"J3MCuPyorqYMnRXxq","product":"Culture"}}},"x/rwDfuBdRbpTgkvxqh":{"finishDate":1412380800,"products":{"a328788326c9ffc983432235a9111315":{"allTypes":[{"_id":"k9LEWTcbmQn53Gzo7","v":3,"name":"dsDNA"},{"v":4,"name":"Plasmid","_id":"cvt43tybpDTWdk2xR"}],"text":"pSB1C3-2Zv2Npu:(LambdaLys-H6) (O352)","type":{"v":4,"name":"Plasmid","_id":"cvt43tybpDTWdk2xR"},"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"pSB1C3-2Zv2Npu:(LambdaLys-H6)","4da843835f15245d46602c3d57b579b6":"223","6f8ac39f837ee4598b7358ce18aec601":"O352"},"cvt43tybpDTWdk2xR":{"c29e15397d29fc94dad70016db7ab2e9":"C"}}}},"values":{"1":{"0":"223"},"0":{"0":"O352"}},"protocol":{"_id":"Nm75fDvHCr38nXJBo","name":"Miniprep","v":2},"params":{"1377cefc182c1de1378c2dbd6e230a20":{"product":"Culture","experiment":"fS8pevAjX5zzW3PpP","v":0,"type":"experiment","text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-H6)"}}},"x/PCHcHCCvfHLLp8sX7":{"finishDate":1412380800,"products":{"a328788326c9ffc983432235a9111315":{"type":{"_id":"cvt43tybpDTWdk2xR","v":4,"name":"Plasmid"},"text":"pSB1C3-2Zv2Npu:(LambdaLys-Rigid) (O353)","allTypes":[{"v":3,"name":"dsDNA","_id":"k9LEWTcbmQn53Gzo7"},{"_id":"cvt43tybpDTWdk2xR","v":4,"name":"Plasmid"}],"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"pSB1C3-2Zv2Npu:(LambdaLys-Rigid)","4da843835f15245d46602c3d57b579b6":"228","6f8ac39f837ee4598b7358ce18aec601":"O353"},"cvt43tybpDTWdk2xR":{"c29e15397d29fc94dad70016db7ab2e9":"C"}}}},"values":{"1":{"0":"228"},"0":{"0":"O353"}},"params":{"1377cefc182c1de1378c2dbd6e230a20":{"product":"Culture","text":"E. coli Turbo with pSB1C3-2Zv2Npu:(LambdaLys-Rigid)","type":"experiment","experiment":"nZTeKbnhiFz65uXuz","v":0}},"protocol":{"_id":"Nm75fDvHCr38nXJBo","name":"Miniprep","v":2}},"x/HBHfxpwN8dfYDxm2p":{"values":{"0":{"0":"O361"},"1":{"0":"273"}},"products":{"a328788326c9ffc983432235a9111315":{"properties":{"cvt43tybpDTWdk2xR":{"c29e15397d29fc94dad70016db7ab2e9":"C"},"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"pSB1C3-LiLaLy(H6)","6f8ac39f837ee4598b7358ce18aec601":"O361","4da843835f15245d46602c3d57b579b6":"273"}},"text":"pSB1C3-LiLaLy(H6) (O361)","type":{"name":"Plasmid","v":4,"_id":"cvt43tybpDTWdk2xR"},"allTypes":[{"_id":"k9LEWTcbmQn53Gzo7","name":"dsDNA","v":3},{"name":"Plasmid","v":4,"_id":"cvt43tybpDTWdk2xR"}]}},"finishDate":1412380800,"protocol":{"_id":"Nm75fDvHCr38nXJBo","name":"Miniprep","v":2},"params":{"1377cefc182c1de1378c2dbd6e230a20":{"text":"E. coli Turbo with pSB1C3-LiLaLy(H6)","type":"experiment","experiment":"Lc5EhnbXEsryy6Bu8","v":0,"product":"Culture"}}},"x/fkKhiMbk9arB3t5kM":{"params":{"64ce87c57c5b9a1a536ccd08a47740b3":{"product":"Agar plate","experiment":"Ljq6u8dsJCLC33QS6","v":0,"text":"E. coli Turbo with pSBX1K3-2Zv2Npu:LaLys+bad1","type":"experiment"}},"protocol":{"v":0,"name":"Pick a colony carrying a plasmid from an agar plate","_id":"maDx7F6ry8Mw5JmKf"},"values":{},"finishDate":1412434020,"products":{"3bbfb159bcd6317e17bc0125824b5a25":{"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+bad1","10990dcf66373fff11ef907bc2b1ef97":"K"}},"allTypes":[{"v":0,"name":"Bacteria","_id":"YW7epnToTmqmHEEwx"},{"_id":"PpBkfrLAqTw2CwNZX","v":2,"name":"Bacteria carrying a plasmid"}],"type":{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"},"text":"E. coli Turbo with pSBX1K3-2Zv2Npu:LaLys+bad1"}}},"x/ZmNTTADyPRvJ8W4X8":{"protocol":{"v":4,"name":"PCR from Bacteria (colony PCR or genomic amplification)","_id":"NgpLzurL2RKFojWWA"},"params":{"dd152b9c02bb725f7af5038f5b1649ac":[{"values":{"2":{"0":"0.375"}},"paramValue":{"text":"VF2 (1)","type":"supply","supply":"usnvt25q5X9ZfqTEL","v":0}},{"paramValue":{"supply":"JPLm2aiw2BAcSbmcY","v":1,"text":"VR (2)","type":"supply"},"values":{"2":{"0":"0.375"}}}],"4c0984e0a4d80995f3099e5c33188ce1":{"product":"Colony","type":"experiment","text":"E. coli Turbo with pSBX1K3-2Zv2Npu:LaLys+bad1","v":0,"experiment":"fkKhiMbk9arB3t5kM"}},"values":{"3":{"0":"2"},"4":{"6":"68","10":300,"3":"30","0":"94","8":"25","4":"52","1":"120","9":72,"5":"30","2":"94","11":"Colony PCR: pSBX**3-2Zv2Npu:LaLys+bad1","7":"180"}},"finishDate":1412437620,"products":{"c3aa9e8a1d807a1fafce9630fe0e797b":{"type":{"_id":"k9LEWTcbmQn53Gzo7","name":"dsDNA","v":3},"text":"Colony PCR: pSBX**3-2Zv2Npu:LaLys+bad1","allTypes":[{"_id":"k9LEWTcbmQn53Gzo7","name":"dsDNA","v":3}],"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"Colony PCR: pSBX**3-2Zv2Npu:LaLys+bad1","4da843835f15245d46602c3d57b579b6":"2","6f8ac39f837ee4598b7358ce18aec601":null}}}}},"x/3LDbDbrSmijmDMCTe":{"protocol":{"_id":"maDx7F6ry8Mw5JmKf","name":"Pick a colony carrying a plasmid from an agar plate","v":0},"params":{"64ce87c57c5b9a1a536ccd08a47740b3":{"v":0,"experiment":"Ljq6u8dsJCLC33QS6","text":"E. coli Turbo with pSBX1K3-2Zv2Npu:LaLys+bad1","type":"experiment","product":"Agar plate"}},"values":{},"products":{"3bbfb159bcd6317e17bc0125824b5a25":{"properties":{"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"K","0ac71517d2d368cdbabf8b9914716902":"pSBX1K3-2Zv2Npu:LaLys+bad1"},"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo"}},"text":"E. coli Turbo with pSBX1K3-2Zv2Npu:LaLys+bad1","type":{"name":"Bacteria carrying a plasmid","v":2,"_id":"PpBkfrLAqTw2CwNZX"},"allTypes":[{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2}]}},"finishDate":1412437620},"x/QRR7uiaGEaBiAkJfu":{"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"text":"E. coli Turbo with pSBX1K3-2Zv2Npu:LaLys+bad1","type":"experiment","experiment":"3LDbDbrSmijmDMCTe","v":0,"product":"Colony"},"dd152b9c02bb725f7af5038f5b1649ac":[{"values":{"2":{"0":"0.375"}},"paramValue":{"text":"VF2 (1)","type":"supply","v":0,"supply":"usnvt25q5X9ZfqTEL"}},{"values":{"2":{"0":"0.375"}},"paramValue":{"v":1,"supply":"JPLm2aiw2BAcSbmcY","type":"supply","text":"VR (2)"}}]},"protocol":{"_id":"NgpLzurL2RKFojWWA","name":"PCR from Bacteria (colony PCR or genomic amplification)","v":4},"finishDate":1412437620,"products":{"c3aa9e8a1d807a1fafce9630fe0e797b":{"type":{"name":"dsDNA","v":3,"_id":"k9LEWTcbmQn53Gzo7"},"text":"Colony PCR: pSBX**3-2Zv2Npu:LaLys+bad1","allTypes":[{"_id":"k9LEWTcbmQn53Gzo7","v":3,"name":"dsDNA"}],"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"Colony PCR: pSBX**3-2Zv2Npu:LaLys+bad1","6f8ac39f837ee4598b7358ce18aec601":null,"4da843835f15245d46602c3d57b579b6":"2"}}}},"values":{"3":{"0":"2"},"4":{"0":"94","10":300,"6":"68","3":"30","7":"180","4":"52","8":"25","11":"Colony PCR: pSBX**3-2Zv2Npu:LaLys+bad1","2":"94","1":"120","9":72,"5":"30"}}},"x/xdTGQbdRrqwzSdCi3":{"finishDate":1412442000,"products":{"12327177bfe077ccd10daa3d5ca9809c":{"properties":{},"allTypes":[{"name":"Agarose Gel","v":0,"_id":"QCyBocmukGKrzbkYb"}],"type":{"v":0,"name":"Agarose Gel","_id":"QCyBocmukGKrzbkYb"},"text":""}},"sid":"433","values":{"11":{"0":"","1":""}},"protocol":{"_id":"JPmjNykwWASQiD9PX","name":"Gel Electrophoresis","v":1},"params":{"5d37875fd1572e9ebc236d502ab9a20d":[{"values":{"10":{"0":"3.0"}},"paramValue":{"v":0,"supply":"PtEdhNN8Q7dYgDbtw","text":"2-log DNA ladder (NEB)","type":"supply"}},{"values":{"10":{"0":""}},"paramValue":{"v":0,"experiment":"2WJNvfiWHQtsXGAwP","type":"experiment","text":"Colony PCR: pSB**3-1GateNpuDnaE(N)*-H6","product":"Amplificate"}},{"paramValue":{"experiment":"xQJusQxh6EjQbKF3z","v":0,"text":"Colony PCR: pSB**3-1GateNpuDnaE(N)*-H6","type":"experiment","product":"Amplificate"},"values":{"10":{"0":""}}},{"paramValue":{"product":"Amplificate","v":0,"experiment":"ZQp2LY56C7NZ6sSyd","type":"experiment","text":"Colony PCR: pSB**3-1GateSspGyrB-S11C_H6"},"values":{"10":{"0":""}}},{"paramValue":{"experiment":"pePWwQbP49gPmF2td","v":0,"type":"experiment","text":"Colony PCR: pSB**3-1GateSspGyrB-S11C_H6","product":"Amplificate"},"values":{"10":{"0":""}}},{"paramValue":{"product":"Amplificate","type":"experiment","text":"Colony PCR: pSB**3-H6-1GateNpu*(C)_v2","experiment":"BKrt87xGhQ5ov22Ke","v":0},"values":{"10":{"0":""}}},{"values":{"10":{"0":""}},"paramValue":{"type":"experiment","text":"Colony PCR: pSB**3-H6-1GateNpu*(C)_v2","experiment":"6jJcG2c843boTPiC2","v":0,"product":"Amplificate"}},{"values":{"10":{"0":""}},"paramValue":{"product":"Amplificate","v":0,"experiment":"DLmHQfb2dwtPTCcbS","text":"Colony PCR: pSB**3-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C)","type":"experiment"}},{"paramValue":{"product":"Amplificate","text":"Colony PCR: pSB**3-1GateNpuDnaE(N)*-H6-H6-1GateNpu*(C)_v2","type":"experiment","experiment":"GXeQbxAdzTtzN3a4x","v":0},"values":{"10":{"0":""}}},{"values":{"10":{"0":""}},"paramValue":{"product":"Amplificate","type":"experiment","text":"Colony PCR: pSB**3-1GateNpuDnaE(N)*-H6-H6-1GateNpu*(C)_v2","v":0,"experiment":"qdHSEEyhi7dXTz5ce"}},{"paramValue":{"text":"Colony PCR: pSB**3-T7RBS_BBv2","type":"experiment","v":0,"experiment":"MJBbAhpwgNMruD9PK","product":"Amplificate"},"values":{"10":{"0":""}}},{"values":{"10":{"0":""}},"paramValue":{"v":0,"experiment":"TsgGjjKjywRmWL8kW","text":"Colony PCR: pSB**3-T7RBS_BBv2","type":"experiment","product":"Amplificate"}},{"paramValue":{"experiment":"ZmNTTADyPRvJ8W4X8","v":0,"text":"Colony PCR: pSBX**3-2Zv2Npu:LaLys+bad1","type":"experiment","product":"Amplificate"},"values":{"10":{"0":""}}},{"paramValue":{"product":"Amplificate","type":"experiment","text":"Colony PCR: pSBX**3-2Zv2Npu:LaLys+bad1","v":0,"experiment":"QRR7uiaGEaBiAkJfu"},"values":{"10":{"0":""}}},{"paramValue":{"text":"2-log DNA ladder (NEB)","type":"supply","supply":"PtEdhNN8Q7dYgDbtw","v":0},"values":{"10":{"0":""}}}]}}}