LOCUS Exported 4002 bp ds-DNA circular SYN 10-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0050_producer_luxI SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4002) AUTHORS Daniel Gerngross TITLE piG0050 JOURNAL Exported 10 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4002 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 50..84 /note="const. promoter (J23100)" RBS 93..104 /note="RBS (B0034)" CDS 111..695 /codon_start=1 /note="luxI (C0161)" /translation="MTIMIKKSDFLAIPSEEYKGILSLRYQVFKQRLEWDLVVENNLES DEYDNSNAEYIYACDDTENVSGCWRLLPTTGDYMLKSVFPELLGQQSAPKDPNIVELSR FAVGKNSSKINNSASEITMKLFEAIYKHAVSQGITEYVTVTSTAIERFLKRIKVPCHRI GDKEIHVLGDTKSVVLSMPINEQFKKAVLN*" terminator 707..793 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" rep_origin 908..1679 /note="pBBR1 oriV" /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" CDS 1680..2342 /codon_start=1 /product="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" /note="pBBR1 Rep" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" oriT 2353..2461 /note="incP origin of transfer" CDS complement(2607..3797) /note="TetR" terminator 3904..3998 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" ORIGIN 1 actagtcgcc agggttttcc cagtcacgac gcggccgcaa gcttgcatgt tgacggctag 61 ctcagtccta ggtacagtgc tagctactag tgaaagagga gaaatactag atgactataa 121 tgataaaaaa atcggatttt ttggcaattc catcggagga gtataaaggt attctaagtc 181 ttcgttatca agtgtttaag caaagacttg agtgggactt agttgtagaa aataaccttg 241 aatcagatga gtatgataac tcaaatgcag aatatattta tgcttgtgat gatactgaaa 301 atgtaagtgg atgctggcgt ttattaccta caacaggtga ttatatgctg aaaagtgttt 361 ttcctgaatt gcttggtcaa cagagtgctc ccaaagatcc taatatagtc gaattaagtc 421 gttttgctgt aggtaaaaat agctcaaaga taaataactc tgctagtgaa attacaatga 481 aactatttga agctatatat aaacacgctg ttagtcaagg tattacagaa tatgtaacag 541 taacatcaac agcaatagag cgatttttaa agcgtattaa agttccttgt catcgtattg 601 gagacaaaga aattcatgta ttaggtgata ctaaatcggt tgtattgtct atgcctatta 661 atgaacagtt taaaaaagca gtcttaaatt aataaattaa aggcatcaaa taaaacgaaa 721 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct 781 gagtaggaca aatccgccgc cctagacagc tgggcgcgcc ccccctacgg gcttgctctc 841 cgggcttcgc cctgcgcggt cgctgcgctc ccttgccagc ccgtggatat gtggacgatg 901 gccgcgagcg gccaccggct ggctcgcttc gctcggcccg tggacaaccc tgctggacaa 961 gctgatggac aggctgcgcc tgcccacgag cttgaccaca gggattgccc accggctacc 1021 cagccttcga ccacataccc accggctcca actgcgcggc ctgcggcctt gccccatcaa 1081 tttttttaat tttctctggg gaaaagcctc cggcctgcgg cctgcgcgct tcgcttgccg 1141 gttggacacc aagtggaagg cgggtcaagg ctcgcgcagc gaccgcgcag cggcttggcc 1201 ttgacgcgcc tggaacgacc caagcctatg cgagtggggg cagtcgaagg gcgaagcccg 1261 cccgcctgcc ccccgagcct cacggcggcg agtgcggggg ttccaagggg gcagcgccac 1321 cttgggcaag gccgaaggcc gcgcagtcga tcaacaagcc ccggaggggc cactttttgc 1381 cggaggggga gccgcgccga aggcgtgggg gaaccccgca ggggtgccct tctttgggca 1441 ccaaagaact agatataggg cgaaatgcga aagacttaaa aatcaacaac ttaaaaaagg 1501 ggggtacgca acagctcatt gcggcacccc ccgcaatagc tcattgcgta ggttaaagaa 1561 aatctgtaat tgactgccac ttttacgcaa cgcataattg ttgtcgcgct gccgaaaagt 1621 tgcagctgat tgcgcatggt gccgcaaccg tgcggcaccc ctaccgcatg gagataagca 1681 tggccacgca gtccagagaa atcggcattc aagccaagaa caagcccggt cactgggtgc 1741 aaacggaacg caaagcgcat gaggcgtggg ccgggcttat tgcgaggaaa cccacggcgg 1801 caatgctgct gcatcacctc gtggcgcaga tgggccacca gaacgccgtg gtggtcagcc 1861 agaagacact ttccaagctc atcggacgtt ctttgcggac ggtccaatac gcagtcaagg 1921 acttggtggc cgagcgctgg atctccgtcg tgaagctcaa cggccccggc accgtgtcgg 1981 cctacgtggt caatgaccgc gtggcgtggg gccagccccg cgaccagttg cgcctgtcgg 2041 tgttcagtgc cgccgtggtg gttgatcacg acgaccagga cgaatcgctg ttggggcatg 2101 gcgacctgcg ccgcatcccg accctgtatc cgggcgagca gcaactaccg accggccccg 2161 gcgaggagcc gcccagccag cccggcattc cgggcatgga accagacctg ccagccttga 2221 ccgaaacgga ggaatgggaa cggcgcgggc agcagcgcct gccgatgccc gatgagccgt 2281 gttttctgga cgatggcgag ccgttggagc cgccgacacg ggtaacgctg ccgcgccggt 2341 agggccggcc tacggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga 2401 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc 2461 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt 2521 atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg 2581 ttatgcagcg gaaaaggaca attgtctcag gtcgaggtgg cccggctcca tgcaccgcga 2641 cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc 2701 catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt 2761 aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc 2821 ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata 2881 atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg 2941 gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg 3001 acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc 3061 gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt 3121 cctacgagtt gcatgataaa gaagacagtc ataagtgcgg ccacaatggt catgccccgc 3181 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggacg gcgctctccc 3241 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc 3301 gccgcaagga atggtgcgtg cagggagatg gcgcccaaca gtcccccggc cacggggcct 3361 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc 3421 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg 3481 gccacgatgc gtccggcgta gagaatccac aggacgggtg tggtcgccat gatcgcgtag 3541 tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac 3601 agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg 3661 ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc 3721 ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc 3781 gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta 3841 ccgcattaca gtttatcgat gataagctgt caaatttaaa tcgtaattat tggggacccc 3901 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg 3961 gagttctgag gtcattactg gatctatcaa caggagtcca ag //