{"x/xnz6NCz6jqecQAm7e":{"protocol":{"_id":"mJNyMCoEko3JKRv5x","v":4,"name":"Heat shock plasmid transformation (0 -> 1 plasmid)"},"params":{"63aa3271e10f79baaba8465140e427e9":{"product":"Plasmid DNA","type":"experiment","text":"pUC19 (O12-3)","v":0,"experiment":"NFugqunpnhgXcmQQq"},"691586db4be0d1aff3ffb8b43b31cc9a":{"product":"Competent cells","text":"E. coli BL21(DE3)","type":"experiment","experiment":"aYRkbZSfBQqLEWT45","v":0}},"values":{"4":{"0":"30"},"6":{"2":37,"1":"60","0":"500"},"8":{"0":""},"3":{"0":"30"},"2":{"0":"2"},"5":{"0":"5"},"0":{"0":"42"}},"products":{"1f90a3d695361444cb4d5bd0bc04c741":{"allTypes":[{"name":"Bacteria","v":0,"_id":"YW7epnToTmqmHEEwx"},{"v":2,"name":"Bacteria carrying a plasmid","_id":"PpBkfrLAqTw2CwNZX"}],"type":{"_id":"PpBkfrLAqTw2CwNZX","v":2,"name":"Bacteria carrying a plasmid"},"text":"E. coli BL21(DE3) with pUC19","properties":{"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"A","0ac71517d2d368cdbabf8b9914716902":"pUC19"},"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)","e1520b5997a532c7889f6e8883920ab8":"E. coli"}}}},"finishDate":1410206400},"x/tZtiQ6GHEZtb6Lqm9":{"params":{"691586db4be0d1aff3ffb8b43b31cc9a":{"v":0,"experiment":"xCYABjkk48sY8uu8h","type":"experiment","text":"E. coli Turbo","product":"Competent cells"},"63aa3271e10f79baaba8465140e427e9":{"v":0,"experiment":"NFugqunpnhgXcmQQq","type":"experiment","text":"pUC19 (O12-3)","product":"Plasmid DNA"}},"protocol":{"v":4,"name":"Heat shock plasmid transformation (0 -> 1 plasmid)","_id":"mJNyMCoEko3JKRv5x"},"values":{"8":{"0":""},"6":{"2":37,"1":"60","0":"500"},"4":{"0":"30"},"5":{"0":"5"},"2":{"0":"2"},"3":{"0":"30"},"0":{"0":"42"}},"products":{"1f90a3d695361444cb4d5bd0bc04c741":{"properties":{"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"A","0ac71517d2d368cdbabf8b9914716902":"pUC19"},"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e1520b5997a532c7889f6e8883920ab8":"E. coli"}},"text":"E. coli Turbo with pUC19","type":{"name":"Bacteria carrying a plasmid","v":2,"_id":"PpBkfrLAqTw2CwNZX"},"allTypes":[{"v":0,"name":"Bacteria","_id":"YW7epnToTmqmHEEwx"},{"name":"Bacteria carrying a plasmid","v":2,"_id":"PpBkfrLAqTw2CwNZX"}]}},"finishDate":1410206400},"x/Hhpm4ueDKgpSmuicC":{"params":{"691586db4be0d1aff3ffb8b43b31cc9a":{"product":"Competent cells","v":0,"experiment":"ZLfH6YbwsGuqrNn9G","text":"E. coli TOP10","type":"experiment"},"63aa3271e10f79baaba8465140e427e9":{"type":"experiment","text":"pUC19 (O12-3)","v":0,"experiment":"NFugqunpnhgXcmQQq","product":"Plasmid DNA"}},"protocol":{"v":4,"name":"Heat shock plasmid transformation (0 -> 1 plasmid)","_id":"mJNyMCoEko3JKRv5x"},"finishDate":1410206400,"products":{"1f90a3d695361444cb4d5bd0bc04c741":{"allTypes":[{"name":"Bacteria","v":0,"_id":"YW7epnToTmqmHEEwx"},{"name":"Bacteria carrying a plasmid","v":2,"_id":"PpBkfrLAqTw2CwNZX"}],"text":"E. coli TOP10 with pUC19","type":{"_id":"PpBkfrLAqTw2CwNZX","v":2,"name":"Bacteria carrying a plasmid"},"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"TOP10","e1520b5997a532c7889f6e8883920ab8":"E. coli"},"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"A","0ac71517d2d368cdbabf8b9914716902":"pUC19"}}}},"values":{"4":{"0":"30"},"6":{"0":"500","2":37,"1":"60"},"8":{"0":""},"3":{"0":"30"},"2":{"0":"2"},"5":{"0":"5"},"0":{"0":"42"}}},"x/32ePpkEMFAukM8oPP":{"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"text":"E. coli BL21(DE3)","type":"experiment","experiment":"sP5kW8nnQ57CuJrY3","v":0,"product":"Glycerol stock"},"87f8a6ab85c9ced3702b4ea641ad4bb5":{"product":"Medium","v":1,"experiment":"aJkHPGccpLkiga4w5","text":"Lysogeny broth + ","type":"experiment"}},"protocol":{"_id":"3cuNZHSvzbmc5wGLz","name":"Make a liquid culture without any plasmid","v":3},"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)"}},"text":"E. coli BL21(DE3)","type":{"_id":"8p9DTRXiSoymBbC3T","v":1,"name":"Bacterial culture without any plasmid"},"allTypes":[{"_id":"nQbZRzR4ZeKkYMr8X","name":"Bacteria without any plasmid","v":0},{"_id":"YW7epnToTmqmHEEwx","name":"Bacteria","v":0},{"_id":"8p9DTRXiSoymBbC3T","name":"Bacterial culture without any plasmid","v":1}]}},"finishDate":1410210000,"values":{"0":{"1":"","0":""},"1":{"1":0,"0":"37"}}},"x/4sXA3SKYQMmgXZTzq":{"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"type":{"_id":"8p9DTRXiSoymBbC3T","name":"Bacterial culture without any plasmid","v":1},"text":"E. coli BL21(DE3)","allTypes":[{"_id":"nQbZRzR4ZeKkYMr8X","v":0,"name":"Bacteria without any plasmid"},{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"8p9DTRXiSoymBbC3T","v":1,"name":"Bacterial culture without any plasmid"}],"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)","e1520b5997a532c7889f6e8883920ab8":"E. coli"}}}},"finishDate":1410210000,"values":{"0":{"0":"","1":""},"1":{"1":0,"0":"37"}},"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"type":"experiment","text":"E. coli BL21(DE3)","v":0,"experiment":"sP5kW8nnQ57CuJrY3","product":"Glycerol stock"},"87f8a6ab85c9ced3702b4ea641ad4bb5":{"v":1,"experiment":"aJkHPGccpLkiga4w5","text":"Lysogeny broth + ","type":"experiment","product":"Medium"}},"protocol":{"name":"Make a liquid culture without any plasmid","v":3,"_id":"3cuNZHSvzbmc5wGLz"}},"x/zPaaXwpXSK6smJFmv":{"values":{},"finishDate":1410213600,"products":{"a0b4e4a2b22f540a0527e129d1f98684":{"properties":{"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)"}},"allTypes":[{"_id":"nQbZRzR4ZeKkYMr8X","name":"Bacteria without any plasmid","v":0},{"_id":"YW7epnToTmqmHEEwx","name":"Bacteria","v":0},{"_id":"Xwooe4QfsLfskZNvG","v":0,"name":"Chemically competent bacteria without any plasmid"}],"text":"E. coli BL21(DE3)","type":{"_id":"Xwooe4QfsLfskZNvG","name":"Chemically competent bacteria without any plasmid","v":0}}},"params":{"0ce97191c161424bd6b9642a75f54aca":{"text":"E. coli BL21(DE3)","type":"experiment","experiment":"4sXA3SKYQMmgXZTzq","v":0,"product":"Culture"}},"protocol":{"name":"Make competent cells not yet carrying any plasmid","v":1,"_id":"F2oNT7tGTyGFCjAB9"}},"x/kre8KPsmt8HhmE4dM":{"params":{"a1610acb29b294b95abcdeb4b9fdd74b":{"product":"Bacteria carrying the plasmid","experiment":"xnz6NCz6jqecQAm7e","v":0,"text":"E. coli BL21(DE3) with pUC19","type":"experiment"}},"protocol":{"v":0,"name":"Streak bacteria carrying a plasmid onto an agar plate","_id":"kxBySpW3SnbWuYMRw"},"finishDate":1410264000,"products":{"fccc3bbdd62f7ea544bf80e802c28b89":{"type":{"_id":"D2DD2v2FYpYQLFwji","name":"Agar plate with bacteria carrying a plasmid","v":0},"text":"E. coli BL21(DE3) with pUC19","allTypes":[{"v":0,"name":"Agar plate with bacteria carrying a plasmid","_id":"D2DD2v2FYpYQLFwji"}],"properties":{"D2DD2v2FYpYQLFwji":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","e4963ca4d4576f5cb6f8af52e3d038b6":"A","0ac71517d2d368cdbabf8b9914716902":"pUC19","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)"}}}},"values":{"0":{"1":"A","0":"80"}}},"x/6JX3yEoN7hQRpvMWp":{"finishDate":1410264000,"products":{"fccc3bbdd62f7ea544bf80e802c28b89":{"properties":{"D2DD2v2FYpYQLFwji":{"0ac71517d2d368cdbabf8b9914716902":"pUC19","1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e4963ca4d4576f5cb6f8af52e3d038b6":"A","e1520b5997a532c7889f6e8883920ab8":"E. coli"}},"text":"E. coli Turbo with pUC19","type":{"v":0,"name":"Agar plate with bacteria carrying a plasmid","_id":"D2DD2v2FYpYQLFwji"},"allTypes":[{"_id":"D2DD2v2FYpYQLFwji","v":0,"name":"Agar plate with bacteria carrying a plasmid"}]}},"values":{"0":{"1":"A","0":"80"}},"protocol":{"name":"Streak bacteria carrying a plasmid onto an agar plate","v":0,"_id":"kxBySpW3SnbWuYMRw"},"params":{"a1610acb29b294b95abcdeb4b9fdd74b":{"experiment":"tZtiQ6GHEZtb6Lqm9","v":0,"type":"experiment","text":"E. coli Turbo with pUC19","product":"Bacteria carrying the plasmid"}}},"x/qrtHdcBuBkG8mTMEh":{"protocol":{"v":0,"name":"Streak bacteria carrying a plasmid onto an agar plate","_id":"kxBySpW3SnbWuYMRw"},"params":{"a1610acb29b294b95abcdeb4b9fdd74b":{"product":"Bacteria carrying the plasmid","v":0,"experiment":"Hhpm4ueDKgpSmuicC","text":"E. coli TOP10 with pUC19","type":"experiment"}},"values":{"0":{"1":"A","0":"80"}},"products":{"fccc3bbdd62f7ea544bf80e802c28b89":{"type":{"name":"Agar plate with bacteria carrying a plasmid","v":0,"_id":"D2DD2v2FYpYQLFwji"},"text":"E. coli TOP10 with pUC19","allTypes":[{"_id":"D2DD2v2FYpYQLFwji","name":"Agar plate with bacteria carrying a plasmid","v":0}],"properties":{"D2DD2v2FYpYQLFwji":{"0ac71517d2d368cdbabf8b9914716902":"pUC19","1ed8ac857097c4fbbbc15cd373ef96bc":"TOP10","e4963ca4d4576f5cb6f8af52e3d038b6":"A","e1520b5997a532c7889f6e8883920ab8":"E. coli"}}}},"finishDate":1410264000}}