LOCUS Exported 3576 bp ds-DNA circular SYN 17-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0060_sensor_prhlRstRBS-sfGFP SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 3576) AUTHORS Daniel Gerngross TITLE Direct Submission JOURNAL Exported 17 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3576 /organism="recombinant plasmid" /mol_type="other DNA" terminator 1..126 /note="T0" terminator 27..121 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" misc_feature 127..196 /note="spacer (B0040)" promoter 197..247 /note="P(Rhl) (I14017)" RBS 256..267 /note="RBS (B0034)" CDS 274..999 /codon_start=1 /note="sfGFP" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYKRP*" terminator 1002..1118 /note="T1" terminator 1011..1097 /gene="Escherichia coli rrnB" /note="T1" /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 1125..1144 /note="AB179" primer_bind 1310..1329 /note="AB5" rep_origin 1463..2290 /direction=RIGHT /note="p15A" rep_origin 1711..1713 /direction=RIGHT /note="P15 ori from pACYC184" rep_origin complement(1714..2258) /direction=LEFT /note="p15A ori" /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." primer_bind 2362..2381 /note="AB180" oriT 2392..2500 /note="incP origin of transfer" CDS complement(2656..3471) /note="KamR" ORIGIN 1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg 61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 121 ccaagaaggt tctgttaagt aactgaaccc aatgtcgtta gtgacgctta cctcttaaga 181 ggtcactgac ctaacatcct gcatgaattg gtaggcttac gcaagaaaat ggtttgttat 241 agtcgaatac tagagaaaga ggagaaatac tagatgagca aaggagaaga acttttcact 301 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc 361 cgtggagagg gtgaaggtga tgctacaaac ggaaaactca cccttaaatt tatttgcact 421 actggaaaac tacctgttcc gtggccaaca cttgtcacta ctctgaccta tggtgttcaa 481 tgcttttccc gttatccgga tcacatgaaa cggcatgact ttttcaagag tgccatgccc 541 gaaggttatg tacaggaacg cactatatct ttcaaagatg acgggaccta caagacgcgt 601 gctgaagtca agtttgaagg tgataccctt gttaatcgta tcgagttaaa gggtattgat 661 tttaaagaag atggaaacat tcttggacac aaactcgagt acaactttaa ctcacacaat 721 gtatacatca cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa aattcgccac 781 aacgttgaag atggttccgt tcaactagca gaccattatc aacaaaatac tccaattggc 841 gatggccctg tccttttacc agacaaccat tacctgtcga cacaatctgt cctttcgaaa 901 gatcccaacg aaaagcgtga ccacatggtc cttcttgagt ttgtaactgc tgctgggatt 961 acacatggca tggatgagct ctacaaaagg ccttaataaa ttaaaggcat caaataaaac 1021 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 1081 tcctgagtag gacaaatccg ccgccctaga cagctgggcg cgccaaatac gcccggtagt 1141 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt 1201 cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct 1261 gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc 1321 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat 1381 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg 1441 acatcagcgc tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt 1501 gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat 1561 acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc 1621 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta 1681 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa 1741 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata 1801 ccaggcgttt cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc 1861 ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg 1921 gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg 1981 cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact 2041 ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag 2101 gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca 2161 aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt 2221 cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt attaatcaga 2281 taaaatattt ctagcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 2341 ggggttccgc gcacatttcc ccgaaaagtg ccacctgcta gggccggcct acggccagcc 2401 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga 2461 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca 2521 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg 2581 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaaggaccc 2641 ttgtccaatt aattattaga aaaattcatc cagcatcaga tgaaattgca gtttgttcat 2701 atccggatta tcaatgccat atttctgaaa cagacgtttt tgcaggctcg ggctaaattc 2761 gcccaggcag ttccacagaa tggccagatc ctgataacga tccgcaatgc ccacacggcc 2821 cacatcaatg cagccaatca gtttgccttc atcgaaaatc aggttatcca ggctaaaatc 2881 gccgtgggtc accacgctat ccgggctaaa cggcagcagt ttatgcattt ctttccacac 2941 ctgttccacc ggccagccgt tacgttcatc atcaaaatcg ctcgcatcca ccaggccgtt 3001 gttcatacgg ctctgcgcct gggccagacg aaacacacga tcgctgttaa acgggcagtt 3061 gcacaccgga atgctatgca gacgacgcag aaacacggcc agcgcatcca caatgttttc 3121 gccgctatcc ggatattctt ccagcacctg aaacgcggtt ttgcccggaa tcgcggtggt 3181 cagcagccac gcatcatccg gggtgcgaat aaaatgttta atggtcggca gcggcataaa 3241 ttcggtcagc cagttcagac gcaccatttc atcggtcaca tcgttcgcca cgctgccttt 3301 gccatgtttc agaaacagtt ccggcgcatc cggtttgcca tacagacgat aaatggtcgc 3361 gccgctctga cccacgttat cacgcgccca tttatagcca tacagatccg catccatgtt 3421 gctgttcaga cgcggacggc tacagctcgt ttcacgctga atatggctca taacacccct 3481 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg 3541 tgcaatgtaa catcagagat tttgagacac aaattt //