LOCUS Exported 3937 bp ds-DNA circular SYN 12-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0046_regulator_luxR(J23109)_corrected SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3937) AUTHORS iGEM ETC Zurich TITLE Direct Submission JOURNAL Exported 12 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3937 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..126 /note="T0" terminator 27..121 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" promoter 165..199 /note="J23109" /note="const. promoter" RBS 208..219 /note="RBS (B0034)" RBS 208..219 /note="RBS (B0034)" CDS 226..981 /codon_start=1 /product="LuxR-Barcode" /note="luxR (C0062)" /translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLAI IYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAVNK KSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPLIVP SLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHLTNAQ MKLNTTNRCQSISKAILTGAIDCPYFKN*" CDS 226..228 /codon_start=1 /note="lasR (C0179)" /translation="M" CDS 976..981 /codon_start=1 /note="lasR (C0179)" /translation="*" terminator 990..1118 /note="B0015" /note="directional double term " terminator 1130..1216 /gene="Escherichia coli rrnB" /note="T1" /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind complement(1236..1262) /note="AB185" CDS 1398..1589 /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /note="rop" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" misc_feature 1398..1589 /note="rop" misc_feature 1691..1831 /note="bom" /note="basis of mobility region from pBR322" rep_origin complement(2002..2621) /direction=LEFT /note="pBR322_origin" rep_origin complement(2017..2605) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2612..2638) /note="AB186" oriT 2641..2749 /note="incP origin of transfer" CDS complement(2939..3799) /note="AmpR" promoter complement(3800..3891) /gene="bla" /note="AmpR promoter" ORIGIN 1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg 61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 121 ccaagactag tcgccagggt tttcccagtc acgacgcggc cgcatttaca gctagctcag 181 tcctagggac tgtgctagct actagagaaa gaggagaaat actagatgaa aaacataaat 241 gccgacgaca catacagaat aattaataaa attaaagctt gtagaagcaa taatgatatt 301 aatcaatgct tatctgatat gactaaaatg gtacattgtg aatattattt actcgcgatc 361 atttatcctc attctatggt taaatctgat atttcaatcc tagataatta ccctaaaaaa 421 tggaggcaat attatgatga cgctaattta ataaaatatg atcctatagt agattattct 481 aactccaatc attcaccaat taattggaat atatttgaaa acaatgctgt aaataaaaaa 541 tctccaaatg taattaaaga agcgaaaaca tcaggtctta tcactgggtt tagtttccct 601 attcatacgg ctaacaatgg cttcggaatg cttagttttg cacattcaga aaaagacaac 661 tatatagata gtttattttt acatgcgtgt atgaacatac cattaattgt tccttctcta 721 gttgataatt atcgaaaaat aaatatagca aataataaat caaacaacga tttaaccaaa 781 agagaaaaag aatgtttagc gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa 841 atattaggtt gcagtgagcg tactgtcact ttccatttaa ccaatgcgca aatgaaactc 901 aatacaacaa accgctgcca aagtatttct aaagcaattt taacaggagc aattgattgc 961 ccatacttta aaaattaata atactagagc caggcatcaa ataaaacgaa aggctcagtc 1021 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 1081 cactggctca ccttcgggtg ggcctttctg cgtttataat taaaggcatc aaataaaacg 1141 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1201 cctgagtagg acaaatccgc cgccctagac agctgggcgc gcccccgccg catccatacc 1261 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt 1321 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaattccccc 1381 ttacacggag gcatcaagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta 1441 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg 1501 cagacatctg tgaatcgctg cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc 1561 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 1621 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 1681 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa 1741 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 1801 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc 1861 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 1921 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 1981 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 2041 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 2101 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 2161 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 2221 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 2281 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 2341 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 2401 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 2461 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 2521 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 2581 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggccggccta 2641 cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt 2701 gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg 2761 ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa 2821 aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga 2881 aaaggacaag ggtcgtccaa aaaaaaaggc tccaaaagga gcctttaatt gtatcggttt 2941 accaatgttt aatcaggctc gcgccaattt ccgcaatctg acggttacgt tcatccatcg 3001 tcgcctggct gccggtggta taaatcacca caatacggct cggtttacca tccgggccca 3061 gcgccgcaat aatgccacgg ctgccacgtt cgcccgcacc gcttttatcc gcaataaacc 3121 agccagccgg cagcgcgcta cgcagcagcg gacccgccac tttatccgct tccatccaat 3181 caatcagttg ctggcggctt gccagggtca gcagctcacc cgtcagcagt ttacgcaggg 3241 tggtcgccat tgctgccggc atggtggtat cacgttcatc gttcggaatc gcttcgttca 3301 gttccggttc ccaacgatcc agacgggtca catgatcgcc catgttatgc agaaacgcgg 3361 tcagttcttt cggaccgcca atggtcgtca gcagcaggtt cgccgcggtg ttatcgctca 3421 tggtaatcgc cgcgctgcac agttcacgca cggtcatgcc atcggtcaga tgtttttcgg 3481 tcaccgggct atattccacc agatcgttct ggctataatg aatacgacgg cccagttgtt 3541 cctggcccgc atccacacgg ctcagaaccg caccgcacag cagcacttta aaggtgctca 3601 tcatcggaaa acgttcttcc ggacgaaaag attccagaat tttgccgctg ttcagatcca 3661 gttcaatata gcccacgcgc gcacccagtt gatcttccgc atctttcact ttcaccaggg 3721 tttccggatg cgcaaacacc ggcaggcaaa acgccgcaaa aaacggaatc agcgccacac 3781 gaaaatgctg aatgctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 3841 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggaat 3901 taaaaaaaag cccgctcatt aggcgggcta ctaattt //