09/30/2014

Tomorrow we’re going to do the final test with the bioaccumulation construction (BB_Essential + K346004). So we discussed the procedures and let the material ready. Besides that, we tested if our construction BB_Essential + BB_Bioremediation grows at higher concentrations beyond the ones tested before (20µg/ml). So we plated those colonies in LM medium on Hg concentrations of 50 µg/ml, 70 µg/ml and 100 µg/ml.

We also continued the validation and characterization of our parts regarding to molecular-genetic. Back again to the rigorous digestions using the following system for BB_Essential e BB_Essential + BB_Bioremediation:

Electrophoretic digestion’s profile of BB_Essential:

1:BB_Essential in PSB1C3 not digested

2: BB_Essential in PSB1C3 digested with EcoRI.

3: BB_Essential in PSB1C3 digested with PstI

4: BB_Essential in PSB1C3 digested with EcoRI and PstI

Electrophoretic digestion’s profile of BB_Essential + BB_Bioremediation:

1: BB_Essential + BB_Bioremediation in PSB1C3 not digested

2: BB_Essential + BB_Bioremediation in PSB1C3 digested with EcoRI

3: BB_Essential + BB_Bioremediation in PSB1C3 digested with PstI

4: BB_Essential + BB_Bioremediation in PSB1C3 digested with EcoRI e PstI