Team:Heidelberg/AwesomeSheet/Strains

From 2014.igem.org

– Strains

ABCDEFGHIJK
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OrganismSuitaBIe forExpresses
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IDSpeciesStrainDescriptioncloningexpressionT7 PolccdA
growth speed
Genotypecomments
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1E. coliTOP10
Invitrogen cloning strain for chemically competent cells
yesnononoslow
F- mcrA Δ( mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ( araleu)7697galU galK rpsL (StrR) endA1 nupG
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2E. coliDH10betaCloning strain for electrocompetent cellsyesnononoslow
Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC)
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3E. coliTurbosNEB Turbos, fast growing cloning strainyesnononomediumF' proA+B+ lacIq ∆lacZM15 / fhuA2 ∆(lac-proAB) glnV galK16 galE15 R(zgb-210::Tn10)TetS endA1 thi-1 ∆(hsdS-mcrB)5
does a lot of recombination
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4E. coliMG1655
This is the "wild type" K-12 strain which was sequenced, and should be used when PCRing genes from the sequenced genome. It also looks very healthy under the microscope -- a dramatic difference from most of the cloning strains, which appear sick.
noyesnonofastF- λ- ilvG- rfb-50 rph-1
most natural strain
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5E. coliOne Shotyesnonoyesslow
F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697galU galK rpsL (StrR) endA1 nupG fhuA::IS2
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6E. coliBI21(DE3)noyesyesnofastfhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5
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7E. coliRosetta (DE3)noyesyesno?F- ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE2 (CamR)
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8Bacillus subtilis168received from Ilka Bischofs
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9E. coliWastethe most abundant strainnonoprobablypossibly
extremely fast
WasTE Grb::age ΔtraSh(craP'-dirT)
grows everywhere
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