Team:Heidelberg/AwesomeSheet/Induktion

From 2014.igem.org

– Induction

	A	D	E	F	G	H	I	J	K	L	M	N	O	P	Q	R	S	T	U	V	W	X	Y	Z	AA	AB	AC
1	ID	name	date	Plasmids				Growth			Induction I				Induction II			Expression					result	comments	OD after PBS preparation:	V for 2ODml	MidnightDoc-ID
2					PID1	PID2	PID3	ID	T	t	OD600	Time	c(n/n)	name	OD600	c(n/n)	name	T	Time	t	OD600	ml for 2 OD*ml
3									°C	h			mM			mM		°C		h

4	1	Silvan	7/29/2014	pSB1AK8-T7-2ZNpu:(GFP-H6)	48			263C1-2	37		0.6		1	IPTG				37		10			pellet was not green
5	2	Charlotte	7/31/2014	pSB1AK8-T7-2ZNpu:(GFP-H6)	48			263C1-2	37	4	0.59		0.4	IPTG				28		5			pellet was not green
6	3	Charlotte	08-02	pSBX4C5-2ZSspH6:GFP	80			299C1	37	5	0.69		0.4	IPTG				28		5			pellet was not green
7	4	Charlotte	08-02	pSBX4C5-2ZSspH6:GFP	80			300C1	37	4	0.58		0.4	IPTG				28		5			pellet was not green
8	5	Charlotte	08-02	pSBX4C5-2ZNpuH6:GFP	81			301C1	37	4	0.56		0.4	IPTG				28		5			pellet was not green
9	6	Charlotte	08-02	pSB1C3-BBa_I746907	43			302C1	37	4	0.55		0.4	IPTG				28		5			pellet was not green
10	7	Charlotte	08-04	pSBX4C5-2ZSspH6:GFP, pRARE	80	31		336C1	37	5	0.63		0.4	IPTG
11	8	Charlotte	08-04	pSBX4C5-2ZNpuH6:GFP, pRARE	81	31		337C1	37	4.5	0.6		0.4	IPTG
12	9	Charlotte	08-04	pSB1C3-BBa_I746907, pRARE	43	31		338C1	37	4	0.62		0.4	IPTG										no growth; transformation seems to have failed
13	10	Lisa + Jan	8/1/2014	pSBX1A3-BBa_I13507, pRARE	57	31		253C1	37				1	IPTG				28		20			liquid was not red	no result
14	11	Lisa + Jan	8/1/2014	pSBX1A3-BBa_I13507, pRARE	57	31		253C2	37				1	IPTG				28		20			liquid was not red
15	12	Lisa + Jan	8/1/2014	pSBX1A3-BBa_I13507, pRARE	57	31		253C3	37				1	IPTG				28		20			liquid was not red
16	13	Lisa + Jan	8/1/2014	pSBX1K3-BBa_I13507, pRARE	59	31		255C1	37				1	IPTG				28		20			liquid was not red
17	14	Lisa + Jan	8/1/2014	pSBX1K3-BBa_I13507, pRARE	59	31		255C2	37				1	IPTG				28		20			liquid was not red
18	15	Lisa + Jan	8/1/2014	pSBX1K3-BBa_I13507, pRARE	59	31		255C3	37				1	IPTG				28		20			liquid was not red
19	16	Lisa + Jan	8/1/2014	pSBX4A5-BBa_I13507, pRARE	61	31		257C1	37				1	IPTG				28		20			liquid was not red
20	17	Lisa + Jan	8/1/2014	pSBX4A5-BBa_I13507, pRARE	61	31		257C2	37				1	IPTG				28		20			liquid was not red
21	18	Lisa + Jan	8/1/2014	pSBX4A5-BBa_I13507, pRARE	61	31		257C3	37				1	IPTG				28		20			liquid was not red
22	19	Anna	08-03	pSBX4C5-BBa_I13507	62			327C1	28		~0.6		1.5	IPTG				28		40			pellet was not red
23	20	Anna	08-03	pSBX4C5-BBa_I13507	62			327C2	28		~0.6		1.5	IPTG				28		40			pellet was red
24	21	Anna	08-03	pSBX4C5-BBa_I13507	62			327C3	28		~0.6		1.5	IPTG				28		40			pellet was not red
25	22	Anna	08-03	pSBX4C5-BBa_I13507	62			327C4	28		~0.6		1.5	IPTG				28		40			pellet was not red
26	23	Anna	08-03	pSBX4C5-BBa_I13507	62			327C5	28		~0.6		1.5	IPTG				28		40			pellet was red
27	24	Anna	08-03	pSBX4C5-BBa_I13507	62			327C6	28		~0.6		1.5	IPTG				28		40			pellet was red
28	25	Jakob	08-04	pSB1C3-BBa_I746907	43			332C1	37		0.65		0.4	IPTG				37		4
29	26	Jakob	08-04	pSB1C3-BBa_I746907	43			333C1	37				0.4	IPTG				37		4
30	27	Charlotte	08-05	pSB1AK8-BBa_I712074-2ZNpuH6:GFP	87			351C1	37		0.59		0	IPTG				37					no induction
31	28	Charlotte	08-05	pSB1AK8-BBa_I712074-2ZSspH6:GFP	86			352C1	37		0		0	IPTG				37		0			maybe Trafo failed
32	29	Charlotte	08-05	pJJDuet30, pSFBAD09	19	20		353C1	37		0.51		0.2 %	L-arabinose		1	IPTG	37		4				time between inductions: 30 mins
33	30	Jan	08-05	pVS41	29			358C	37	13		15:30		IPTG				28						sprayed 200 µL 100 mM IPTG over the plate
34	31	Jan	08-05	pVS07	30			359C	37	13		15:30		IPTG				28						spreyed 200 µL 100 mM IPTG over the plate
35	32	Jan	08-05	pVS41, pHYRSF1	29	17		360C	37	13		15:30		IPTG				28						spreyed 200 µL 100 mM IPTG over the plate
36	33	Jan	08-05	pVS41, pVS07	29	30		361C	37	13		15:30		IPTG				28						spreyed 200 µL 100 mM IPTG over the plate
37	34	Charlotte	08-05	pJJDuet30	19			354C1	37				1	IPTG				37		4
38	35	Charlotte	08-05	pSFBAD09	20			355C1	37		0.61	22:30	0.2 %	L-arabinose				37		4
39	36	Charlotte	08-05	pSB1AK8-BBa_I712074-2ZSspH6:GFP	86			352C3	37		0.42	0:30	1	IPTG				37		4
40	37	Jan	08-06	pHYRSF1	17			356C1-2	28		0.79	16:02	0.4	IPTG				28		16
41	38	Jan	08-06	pHYRSF1-02	18			357C1-2	28		0.64	15:52	0.4	IPTG				28		16
42	39	Jan	08-06	pVS41	29			358C1-2	28		0.72	17:50	0.4	IPTG				28		16			green
43	40	Jan	08-06	pVS07	30			359C1-2	28		0.81	16:21	0.4	IPTG				28		16			green
44	41	Jan	08-06	pVS41, pHYRSF1	29	17		360C1-2	28		0.84	16:18	0.4	IPTG				28		16			green
45	42	Jan	08-06	pVS41, pVS07	29	30		361C1-2	28		0.67	15:52	0.4	IPTG				28		16			green
46	43	Charlotte	08-06	pSB1C3-BBa_I746907	43			371C1	37		0.6		1	IPTG				37					hopefully green
47	44	Charlotte	08-06	pSB1AK8-BBa_I712074-2ZSspH6:GFP	86			372C1	37		0.6		1	IPTG				37		4
48	45	Charlotte	08-06	pSB1AK8-BBa_I712074-2ZNpuH6:GFP	87			351C2	37		0.6		1	IPTG				37		4
49	46	Anna	08-07	pSBX1K3-BBa_I13507	59			384C1	28					IPTG									red
50	47	Anna	08-07	pSBX1K3-BBa_I13507	59			384C2	28					IPTG									red
51	48	Anna	08-07	pSBX1K3-BBa_I13507	59			384C3	28					IPTG									red
52	49	Anna	08-07	pSBX1K3-BBa_I13507	59			385C1	28					IPTG									red
53	50	Anna	08-07	pSBX1K3-BBa_I13507	59			385C2	28					IPTG									red
54	51	Anna	08-07	pSBX1K3-BBa_I13507	59			385C3	28					IPTG									red
55	52	Anna	08-07	pSBX4A5-BBa_I13507	61			386C1	28					IPTG									negativ
56	53	Anna	08-07	pSBX4A5-BBa_I13507	61			386C2	28					IPTG									red
57	54	Anna	08-07	pSBX4A5-BBa_I13507	61			386C3	28					IPTG									red
58	55	Anna	08-07	pSBX4K5-BBa_I13507	63			387C1	28					IPTG									negativ
59	56	Anna	08-07	pSBX4K5-BBa_I13507	63			387C2	28					IPTG									red
60	57	Anna	08-07	pSBX4K5-BBa_I13507	63			387C3	28					IPTG									red
61	58	Anna	08-08	pSBX1C3-BBa_I13507	58			383C1	28					IPTG
62	59	Anna	08-08	pSBX1C3-BBa_I13507	58			383C2	28					IPTG
63	60	Anna	08-08	pSBX1C3-BBa_I13507	58			383C3	28					IPTG
64	61	Charlotte	08-10	pSB1AK8-T7-2ZNpu:(GFP-H6)	48			263C1-3	37		0.64		1	IPTG				37		4			green
65	62	Charlotte	08-10	pSB1AK8-T7-2ZNpu:(GFP-H6)	48			263C1-4	37		0.63		1	IPTG				28		4			green
66	63	Charlotte	08-10	pSB1AK8-BBa_I712074-2ZSspH6:GFP	86			372C2	37		0.71		1	IPTG				37		5			not green
67	64	Charlotte	08-10	pSB1AK8-BBa_I712074-2ZSspH6:GFP	86			372C3	37		0.68		1	IPTG				28		5			not green
68	65	Charlotte	08-10	pSBX4C5-2ZSspH6:GFP	80			300C2	37		0.79		1	IPTG				37		4.5				no growth
69	66	Charlotte	08-10	pSBX4C5-2ZSspH6:GFP	80			300C3	37		0.8		1	IPTG				28		4				no growth
70	67	Anna	08-12	pSBX1T3-BBa_I13507	60			417C1	37		0.6			IPTG				28
71	68	Anna	08-12	pSBX1T3-BBa_I13507	60			417C2	37		0.6			ctrl				28
72	69	Anna	08-12	pSBX1T3-BBa_I13507	60			418C1	37		0.6			IPTG				28
73	70	Anna	08-12	pSBX1T3-BBa_I13507	60			418C2	37		0.6			ctrl				28
74	71	Anna	08-12	pSBX1T3-BBa_I13507	60			419C1	37		0.6			IPTG				28
75	72	Anna	08-12	pSBX1T3-BBa_I13507	60			419C2	37		0.6			ctrl				28
76	73	Anna	08-12	pSB1T3-BBa_J04450	64			225C1	37		0.6			pos.ctrl				28
77	74	Jakob	08-13	pSB4A5-BBa_J04450, pRARE	50	31		420C1-1	37		n.i.		0.4	pos. ctrl.				37		4			red even before induction :-P
78	75	Jakob	08-13	pSBX4A5-BBa_I13507, pRARE	61	31		421C1-1	37		n.i.		0.4	ind.				37		4
79	76	Jakob	08-16	pSB4A5-BBa_J04450, pRARE	50	31		420C1-2	37		0.6		0.4	ctrl.				37
80	77	Jakob	08-16	pSBX4A5-BBa_I13507, pRARE	61	31		421C1-2	37		0.6		0.4	ind.				37
81	78	Jan	08-17	pSB1C3-BBa_I746908	104			434C1-2	37	0			0	L-arabinose				37					-
82	79	Jan	08-17	pSB1C3-BBa_I746908	104			434C1-3	37	0			6.67	L-arabinose				37					slightly green
83	80	Jan	08-17	pSB1C3-BBa_I746908	104			434C1-4	37	0			66.67	L-arabinose				37					slightly green
84	81	Jan	08-17	pSBX4A5-BBa_I13507, pRARE	61	31		421C1-3	37	0.5			0	IPTG				37					-
85	82	Jan	08-17	pSBX4A5-BBa_I13507, pRARE	61	31		421C1-4	37	0.5			0.1	IPTG				37					-
86	83	Jan	08-17	pSBX4A5-BBa_I13507, pRARE	61	31		421C1-5	37	0.5			1	IPTG				37					-
87	84	Charlotte	08-17	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	102			436C1-1	37	4	0.78		1	IPTG				37		12
88	85	Anna	08-20	pSBX1C3-BBa_I13507	58			383C4	37		~0.6		0.10%	IPTG				28
89	86	Anna	08-20	pSBX1C3-BBa_I13507	58			383C5	37		~0.6		0.10%	IPTG				28
90	87	Anna	08-20	pSBX1C3-BBa_I13507	58			383C6	37		~0.6		0.10%	IPTG
91	88	Anna	08-20	pSBX1C3-BBa_I13507	58			383C7	37		~0.6		0.10%	IPTG
92	89	Anna	08-20	pSBX1C3-BBa_I13507	58			383C8	37		~0.6		0.10%	IPTG
93	90	Anna	08-20	pSBX1T3-BBa_I13507	60			418C6	37		~0.6		0.10%	IPTG
94	91	Anna	08-20	pSBX1T3-BBa_I13507	60			418C7	37		~0.6		0.10%	IPTG
95	92	Anna	08-20	pSBX1T3-BBa_I13507	60			418C8	37		~0.6		0.10%	IPTG
96	93	Anna	08-20	pSBX1T3-BBa_I13507	60			418C9	37		~0.6		0.10%	IPTG
97	94	Anna	08-20	pSBX1T3-BBa_I13507	60			418C10	37		~0.6		0.10%	IPTG
98	95	Anna	08-20	pSBX1T3-BBa_I13507	60			417C3	37		~0.6		0.10%	IPTG
99	96	Anna	08-20	pSBX1K3-BBa_I13507	59			384C1-1	37		~0.6		0.10%	IPTG
100	97	Charlotte	08-20	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	102			436C1-4	37		0.89		1	IPTG				37		4
101	98	Charlotte	08-20	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	102			436C1-5	37		0.87		1	IPTG				37		4
102	99	Charlotte	08-20					6C1-4	37		1.5													for reference
103	100
104	101	Jan	08-20	pSB1C3-BBa_I746908	104			434C1-6	37		0.62	15:53	0.20%	L-arabinose									weak, but clear green
105	102	Charlotte	8/22/2014	pSB1AK8-BBa_I712074-2ZSspH6:GFP	86			499C2										37		4.3			not green
106	103	Charlotte	8/22/2014	pSB1AK8-BBa_I712074-2ZSspH6:GFP	86			510C2										37		4.3			not green
107	104	Anna	8/24/2014	pSB1AK8-BBa_I712074-S-BBa_J70390	41			156C2	37		0.75		0.10%	IPTG				28
108	105	Anna	8/24/2014	pSBX4C5-BBa_I13507	62			327C5	37		0.75		0.10%	IPTG				28
109	106	Anna	8/24/2014	pSBX1K3-BBa_I13507	59			384C2	37		0.75		0.10%	IPTG				28
110	107	Anna	8/24/2014	pSBX4A5-BBa_I13507	61			386C2	37		0.75		0.10%	IPTG				28
111	108	Anna	8/24/2014	pSBX4K5-BBa_I13507	63			387C2	37		0.75		0.10%	IPTG				28
112	109	Charlotte	8/21/2014	pSB1AK8-BBa_I712074-2ZSspH6:GFP	86			500C2	37		0.69		1	IPTG				28		4
113	110	Charlotte	8/21/2014	pSB1AK8-BBa_I712074-2ZSspH6:GFP	86			500C2	37		0.71		1	IPTG				37		4
114	111	Charlotte	8/21/2014	pSB1AK8-BBa_I712074-2ZSspH6:GFP	86			510C2	37		0.78		1	IPTG				28		4
115	112	Charlotte	8/21/2014	pSB1AK8-BBa_I712074-2ZSspH6:GFP	86			510C2	37		0.87		1	IPTG				37		4
116	113	Caro	8/27/2014	pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6	105			536C1-1	37		0.63	20:15	1	IPTG				37		4
117	114	Caro	8/27/2014	pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6	106			537C1-1	37		0.5	20:15	1	IPTG				37		4
118	115	Caro	8/27/2014	pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6	107			538C1-1	37		0.77	20:15	1	IPTG				37		4
119	116	Caro	8/27/2014	pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6	108			539C1-1	37		0.61	20:15	1	IPTG				37		4
120	117	Caro	8/27/2014	pSBX1K3-T7RBS-H6-sfGFP-H6	128			540C1-1	37		0.73	20:15	1	IPTG				37		4
121	118	Caro	8/27/2014	pSBX1K3-T7RBS-H6-sfGFP-H6	128			540C2-1	37		0.67	20:15	1	IPTG				37		4
122	119	Caro	8/28/2014	pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC	129			552C2	37		0.66	22:00	1	IPTG				37		3
123	120	Caro	8/28/2014	pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC	129			552C3	37		0.65	22:00	1	IPTG				37		5
124	121	Caro	8/28/2014	pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC	129			552C4	37		0.6	22:00	1	IPTG				RT		3
125	122	Caro	8/28/2014	pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC	129			552C5	37		0.71	22:00	1	IPTG				RT		5
126	123	Caro	8/29/2014	pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6	105			536C2-2	37		0.67	12:45	1	IPTG				37		4	2.2
127	124	Caro	8/29/2014	pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6	106			537C2-2	37		0.94	12:45	1	IPTG				37		4	3.7
128	125	Caro	8/29/2014	pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6	107			538C2-2	37		0.56	12:45	1	IPTG				37		4	1.3
129	126	Caro	8/29/2014	pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6	108			539C2-2	37		0.66	15:00	1	IPTG				37		4	2.9
130	127	Caro	8/29/2014	pSBX1K3-T7RBS-H6-sfGFP-H6	128			540C2-4	37		0.77	12:45	1	IPTG				37		4	1.9
131	128	Caro	8/29/2014	pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC	129			552C2-2	37		0.88	12:45	1	IPTG				37		4	4.1
132	129	Anna	8/29/2014	pSBX4C5-BBa_I13507	62			327C5-2	37		0.56	1:00	1	IPTG				37		2, 12, 24				Expression test of pSBX; Samples were taken after 2 h, 12 h, 24 h.
133	130	Anna	8/29/2014	pSBX4C5-BBa_I13507	62			327C5-3	37		0.56	1:00	1	IPTG				37		2, 12, 24				Expression test of pSBX; Samples were taken after 2 h, 12 h, 24 h.
134	131	Anna	8/29/2014	pSBX1K3-BBa_I13507	59			384C3-1	37		0.56	0:00	1	IPTG				37		2, 12, 24				Expression test of pSBX; Samples were taken after 2 h, 12 h, 24 h.
135	132	Anna	8/29/2014	pSBX4A5-BBa_I13507	61			386C2-2	37		0.56	0:00	1	IPTG				37		2, 12, 24				Expression test of pSBX; Samples were taken after 2 h, 12 h, 24 h.
136	133	Anna	8/29/2014	pSBX4K5-BBa_I13507	63			387C2-2	37		0.56	0:00	1	IPTG				37		2, 12, 24				Expression test of pSBX; Samples were taken after 2 h, 12 h, 24 h.
137	134	Anna	8/29/2014	pSBX1T3-BBa_I13507	60			417C3-2	37		0.56	19:00	1	IPTG				37		2, 12, 24				Expression test of pSBX; Samples were taken after 2 h, 12 h, 24 h.
138	135	Anna	8/29/2014	pSB4A5-BBa_J04450, pRARE	50	31		420C6	37									37		2, 12, 24				positive control of pSBX expression test
139	136	Anna	8/29/2014					12C1-2	37									37		2, 12, 24				negative control of pSBX expression test
140	137	Charlotte	8/31/2014	pSB1AK8-T7-2ZNpu:(GFP-H6)	48			263C1-6	37		0.93		1	IPTG				37		10
141	138	Charlotte	8/31/2014	pSB1AK8-BBa_I712074-2ZSspH6:GFP	86			510C2-1	37		0.92		1	IPTG				37		10
142	139	Charlotte	9/1/2014	pSB1C3-BBa_I746907	43			591C2	37		0.71		1	IPTG				37		10
143	140	Charlotte	9/1/2014	pSB1AK8-T7-2ZNpu:(GFP-H6)	48			263C1-7	37		0.72		1	IPTG				37		10
144	141	Charlotte	9/1/2014	pSB1AK8-BBa_I712074-2ZSspH6:GFP	86			510C2-2	37		0.7		1	IPTG				37		10
145	142	Charlotte	9/4/2014	pSBX1K3-2Zv2Npu:(LambdaLys-H6)	110			597C1-1	37		0.7		1	IPTG
146	143	Charlotte	9/4/2014	pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)	111			596C1-1	37		0.73		1	IPTG
147	144	Charlotte	9/7/2014	pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)	111			596C1-2	37				1	IPTG				37		4
148	145	Charlotte	9/7/2014	pSBX1K3-2Zv2Npu:(LambdaLys-H6)	110			597C1-2	37		0.71		1	IPTG				37		4
149	146	CARO	9/2/2014	pSBX1K3-T7RBS-H6-sfGFP-H6	128			540C4	37		0.82	18:00	1	IPTG						3.5				OD after 3.5 hours: 1.8	OD after preparation in PBS: 0.46x15	1.80	0.46	0.2555555556
150	147	CARO	9/2/2014	pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6	105			660C1	37		0.73	18:00	1	IPTG						3.5				OD after 3.5 hours: 1.22	OD after preparation in PBS: 0.5x15	1.22	0.5	0.4098360656
151	148	CARO	9/2/2014	pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6	106			661C1	37		0.71	18:00	1	IPTG						3.5				OD after 3.5 hours: 1.38	OD after preparation in PBS:0.47x15	1.38	0.47	0.3405797101
152	149	CARO	9/2/2014	pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6	107			662C1	37		0.5	18:00	1	IPTG						3.5				OD after 3.5 hours: 1.11	OD after preparation in PBS: 0.46x15	1.11	0.46	0.4144144144
153	150	CARO	9/2/2014	pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6	108			663C1	37		0.5	18:00	1	IPTG						3.5				OD after 3.5 hours: 1.66	OD after preparation in PBS: 0.62x15	0.17	0.62	3.734939759
154	151	CARO	9/2/2014	pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC	129			552C2-3	37		0.65	18:00	1	IPTG						3.5				OD after 3.5 hours: 2.06	OD after preparation in PBS: 0.88x15	2.06	0.88	0.427184466
155	152	CARO	9/2/2014	pSBX1K3-sfGFPN-NpuDnaEN-C->G_NpuDnaEC-sfGFPC-SN->AG	130			601C1-1	37		0.57	18:00	1	IPTG						3.5				OD after 3.5 hours: 1.32	OD after preparation in PBS: 0.45x15	1.32	0.45	0.3409090909
156	153	Charlotte	9/8/2014	pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)	111			596C1-4	37		0.7		1	IPTG				37		4
157	154	Charlotte	9/8/2014	pSBX1K3-2Zv2Npu:(LambdaLys-H6)	110			597C1-4	37		0.75		1	IPTG				37		4
158	155	Charlotte	9/9/2014					6C1-12	37		0.64		0					37		4
159	156	Charlotte	9/9/2014	pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)	111			596C1-5	37		0.69		1	IPTG				37		4
160	157	Charlotte	9/9/2014	pSBX1K3-2Zv2Npu:(LambdaLys-H6)	110			597C1-5	37		0.78		1	IPTG				37		4
161	158	Charlotte	9/9/2014	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	102			436C1-8	37		0.66		1	IPTG				37		4
162	159	Anna	9/4/2014	pSB1AK8-BBa_I712074-BBa_I13507	133			628C1	37		0.6	18:00	1	IPTG				27		2,12				positive control of expression vector test
163	160	Charlotte	9/14/2014					6C1-14	37		0.66							37		4
164	161	Charlotte	9/14/2014	pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)	111			596C1-6	37		0.62		1	IPTG				37		4
165	162	Charlotte	9/14/2014	pSBX1K3-2Zv2Npu:(LambdaLys-H6)	110			597C1-6	37		0.64		1	IPTG				37		4
166	163	Charlotte	9/14/2014	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	102			436C1-10	37		0.61		1	IPTG				37		4
167	164	Charlotte	9/15/2014	pSBX1K3-LiLaLy(H6)	188			733C1	37		0.65		1	IPTG				37		5
168	165	Caro	9/15/2014	pSBX1K3-T7RBS-H6-sfGFP_T65C-H6	180			693C1-2	37		0.65		1	IPTG				37		3	1.2				8
169	166	Caro	9/15/2014	pSBX1K3-T7RBS-H6-sfGFP-H6	128			540C4-1	37		0.72		1	IPTG				37		3	1.2				9
170	167	Caro	9/15/2014	pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6	105			660C1-1	37		0.72		1	IPTG				37		3	2.5				6
171	168	Caro	9/15/2014	pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6	106			661C1-1	37		0.61		1	IPTG				37		3	1.8				8
172	169	Caro	9/15/2014	pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6	107			662C1-1	37		0.67		1	IPTG				37		3	1.2				10
173	170	Caro	9/15/2014	pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6	108			663C1-1	37		0.54		1	IPTG				37		3	1.1				12
174	171	Caro	9/15/2014	pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC	129			552C2-4	37		0.71		1	IPTG				37		3	1.3				12
175	172	Caro	9/15/2014	pSBX1K3-sfGFPN-NpuDnaEN-C->G_NpuDnaEC-sfGFPC-SN->AG	130			601C1-2	37		0.7		1	IPTG				37		3	1.4				8
176	173	Charlotte	9/16/2014	pSBX4C5-2ZSspH6:GFP	80			279C1-7	37		0.65		1	IPTG				37		4
177	174	Charlotte	9/16/2014	pSBX1K3-LiLaLy(H6)	188			733C2	37		0.7		1	IPTG				37		4
178	175	Caro	9/16/2014	pSB4C5-GFP_contr	206			781C1-1	37		0.8		1	IPTG				37		4.5					5.8	344.83	36.25
179	176	Caro	9/16/2014	pSB4C5-mCherry_contr	205			784C1-1	37		0.76		1	IPTG				37		4.5					4.2	476.19	26.25
180	177	Caro	9/16/2014	pSBX4C5-mRFPn1-154_mRFPc155-255_coex	182			736C1-1	37		0.8		1	IPTG				37		4.5	1.5	1,333.33			5.2	384.62	32.5
181	178	Caro	9/16/2014	pSBX4C5-mRFPn1-168-mRFPc169-255_coex	183			738C1-1	37		0.8		1	IPTG				37		4.5	1.9	1,052.63			5.6	357.14	35
182	179	Caro	9/16/2014	pSBX4C5mCherryN_mCherryC_coex	186			740C1-1	37		0.7		1	IPTG				37		4.5	1.7	1,176.47			4.6	434.78	28.75
183	180	Caro	9/16/2014	pSBX4C5-GFPn1-157_GFPc158-255_coex	187			744C2-1	37		0.5		1	IPTG				37		4.5	1.6	1,250.00			3	666.67	18.75
184	181	Caro	9/16/2014	pSB1C3-BBa_J04450	65			700C1-1	37		0.9		1	IPTG				37		4.5	2.2	909.09			4.6	434.78	28.75
185	182	Caro	9/16/2014	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6	175			678C1-1	37									37			1.5	1,333.33			4.8	416.67	30
186	183	Charlotte	9/18/2014	pSBX1K3-2Zv2Npu:(LambdaLys-H6)	110			597C1-8	37		71	1:20						37	5:20	4	2.64	757.58
187	184	Consti	9/21/2014	pSBX1K3-Xylanase	181			692C1-1	37													#DIV/0!
188	185	Consti	9/21/2014	pSBX1K3-XylaH6BB	216			795C2-1	37													#DIV/0!
189	186	Consti	9/21/2014	pSBX1K3-XylaH6extBB	217			796C1-1	37													#DIV/0!
190	187	Consti	9/21/2014	pSBX1K3-2Zv2Npu:Xyla_circ_noHis	113			611C1-1	37		0.58		1	IPTG				20		5		#DIV/0!
191	188	Consti	9/21/2014	pSBX1K3-2Zv2Npu:Xyla(H6)destrCext	124			598C1-1	37													#DIV/0!
192	189	Consti	9/21/2014	pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His	112			563C1-1	37		0.58		1	IPTG				20		5		#DIV/0!
193	190	Consti	9/21/2014	#VALUE!	#VALUE!	#VALUE!	#VALUE!	726-3	37		.		1	IPTG				20				#DIV/0!
194	191	Consti	9/21/2014	pSBX1K3-2Zv2Npu:Xyla(H6)destrCext	124			598C2-1	37		0.65	14:40	1	IPTG				20				#DIV/0!
195	192	Consti	9/21/2014	pSBX1K3-Xylanase	181			840C1-2	37		0.95	15:00	1	IPTG				20				#DIV/0!
196	193	Consti	9/21/2014	pSBX1K3-XylaH6BB	216			841C1-2	37		0.62	15:00	1	IPTG				20				#DIV/0!
197	194	Consti	9/21/2014	pSBX1K3-XylaH6extBB	217			842C1-2	37		0.6	15:00	1	IPTG				20				#DIV/0!
198	195	Charlotte	9/23/2014					6C1-16	37		~0.6	16:00	1	IPTG				37		4		#DIV/0!
199	196	Charlotte	9/24/2014	pSBX1K3-Xylanase	181			840C1-3	37		~0.6	17:00	1	IPTG				37		4		#DIV/0!
200	197	Charlotte	9/25/2014	pSBX1K3-XylaH6BB	216			841C1-3	37		~0.8	18:00	1	IPTG				37		4		#DIV/0!
201	198	Charlotte	9/26/2014	pSBX1K3-XylaH6extBB	217			923C1	37		~0.6	19:00	1	IPTG				37		4		#DIV/0!
202	199	Charlotte	9/27/2014	pSBX1K3-2Zv2Npu:Xyla_circ_noHis	113			611C1-2	37		~0.6	20:00	1	IPTG				37		4		#DIV/0!
203	200	Charlotte	9/28/2014	pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His	112			563C1-2	37		~0.6	21:00	1	IPTG				37		4		#DIV/0!
204	201	Charlotte	9/29/2014	pSBX1K3-2Zv2Npu:Xyla(H6)destrCext	124			598C1-2	37		~0.6	22:00	1	IPTG				37		4		#DIV/0!
205	202	Silvan	9/24/2014	pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6, pRARE	179		3.10E+01	828C1-3	37		0.69	21:50	1	IPTG				15		16	0.45	4,444.44		cooled to 15 °C at 31:20 (OD600 = 0.58)
206	203	Charlotte	9/25/2014	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	102			436C1-11	37		~0.6		1	IPTG				37		4		#DIV/0!
207	204	Charlotte	9/25/2014	pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)	111			596C1-7	37		~0.6		1	IPTG				37		4		#DIV/0!
208	205	Charlotte	9/25/2014	pSBX1K3-2Zv2Npu:(LambdaLys-H6)	110			597C1-9	37		~0.6		1	IPTG				37		4		#DIV/0!
209	206	Charlotte	9/25/2014	pSBX1K3-LiLaLy(H6)	188			733C2-2	37		~0.6		1	IPTG				37		4		#DIV/0!
210	207	Silvan	9/26/2014	pSBX1K3-T7RBS-2ZSortA:eGFP-H6	239			955C1-1	37		0.6	2:20	1	IPTG				37				#DIV/0!
211	208	Silvan	9/28/2014	pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT), pRARE	250		3.10E+01	978C1-1	15	0.5	0.78	21:50	1	IPTG				15			0.7	2,857.14		cooled to 15 °C at 21:15 (OD600 = 0.58)
212	209	Silvan	9/28/2014	pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT), pRARE	250		3.10E+01	978C1-2	15	0.5	0.8	21:50	1	IPTG				15			0.7	2,857.14		cooled to 15 °C at 21:15 (OD600 = 0.62)
213	210	Silvan	9/28/2014	pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT), pRARE	254		3.10E+01	1001C1-1	15	0.5	0.75	7:00	1	IPTG				15		16	0.5	4,000.00		cooled to 15 °C at 06:30 (OD600 = 0.62)
214	211	Silvan	9/28/2014	pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT), pRARE	254		3.10E+01	1001C1-2	15	0.5	0.73	7:00	1	IPTG				15		16	0.5	4,000.00		cooled to 15 °C at 06:30 (OD600 = 0.64)
215	212	Silvan	9/28/2014	pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H6, pRARE	265		3.10E+01	1002C1-1	15	0.5	0.77	7:00	1	IPTG				15		16	0.5	4,000.00		cooled to 15 °C at 06:30 (OD600 = 0.6)
216	213	Consti	9/28/2014	pSBX1K3-Xylanase	181			840C1-4			0.58		1	IPTG				37		4		#DIV/0!
217	214	Consti	9/28/2014	pSBX1K3-2Zv2Npu:Xyla_circ_noHis	113			611C1-3			0.65		1	IPTG				37		4		#DIV/0!
218	215	Consti	9/28/2014	pSBX1K3-XylaH6BB	216			841C1-4			0.51		1	IPTG				37		4		#DIV/0!
219	216	Consti	9/28/2014	pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His	112			563C1-3			0.72		1	IPTG				37		4		#DIV/0!
220	217	Consti	9/28/2014	pSBX1K3-XylaH6extBB	217			923C2-1			0.61		1	IPTG				37		4		#DIV/0!
221	218	Consti	9/28/2014	pSBX1K3-2Zv2Npu:Xyla(H6)destrCext	124			598C1-5			0.59		1	IPTG				37		4		#DIV/0!
222	219	Silvan		pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/4r-FLAG)-H6, pRARE	253		3.10E+01	980C1-1					1	IPTG				15				#DIV/0!
223	220	Silvan		pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT), pRARE	232		3.10E+01	954C1-1					1	IPTG				15				#DIV/0!
224	221	Silvan		pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT), pRARE	232		3.10E+01	954C1-2					1	IPTG				15				#DIV/0!
225	222	Consti	9/30/2014	pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His	112			563C1-6			0.76	18:30	1	IPTG				37				#DIV/0!
226	223	Consti	9/30/2014	pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His	112			563C1-7			0.66	18:30	1	IPTG				37				#DIV/0!
227	224	Consti	9/30/2014	pSBX1K3-XylaH6BB	216			841C1-7			0.63	19:00	1	IPTG				37				#DIV/0!
228	225	Consti	9/30/2014	pSBX1K3-XylaH6BB	216			841C1-8			0.54	19:00	1	IPTG				37				#DIV/0!
229	226	Charlotte	9/30/2014	pSBX1K3-2Zv2Npu:LaLys+may1	285			1044C1			0.6		1	IPTG				37	4			#DIV/0!
230	227	Charlotte	9/30/2014	pSBX1K3-2Zv2Npu:LaLys+sgt2	287			1046C1			0.69		1	IPTG				37	4			#DIV/0!
231	228	Charlotte	9/30/2014	pSBX1K3-2Zv2Npu:LaLys+sho1	288			1047C1			0.69		1	IPTG				37	4			#DIV/0!
232	229	Charlotte	9/30/2014	pSBX1K3-2Zv2Npu:LaLys+sho2	289			1048C1			0.7		1	IPTG				37	4			#DIV/0!
233	230	Charlotte	9/30/2014	pSBX1K3-2Zv2Npu:LaLys+flexlinker	290			1049C1			0.7		1	IPTG				37	4			#DIV/0!
234	231	Charlotte	9/30/2014	pSBX1K3-Xylanase	181			840C1-5			0.72		1	IPTG				37	4			#DIV/0!
235	232	Charlotte	9/30/2014	pSBX1K3-XylaH6BB	216			841C1-5			0.7		1	IPTG				37	4			#DIV/0!
236	233	Charlotte	9/30/2014	pSBX1K3-2Zv2Npu:Xyla_circ_noHis	113			611C1-4			0.6		1	IPTG				37	4			#DIV/0!
237	234	Charlotte	10/2/2014	pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6	105			536C1-4			0.62		1	IPTG				37	4			#DIV/0!
238	235	Charlotte	10/2/2014	pSBX1K3-2Zv2Npu:LaLys+may1	285			1044C1-2			~0.6		1	IPTG				37	4			#DIV/0!
239	236	Charlotte	10/2/2014	pSBX1K3-2Zv2Npu:LaLys+sgt2	287			1046C1-2			~0.6		1	IPTG				37	4			#DIV/0!
240	237	Charlotte	10/2/2014	pSBX1K3-2Zv2Npu:LaLys+sho1	288			1047C1-2			~0.6		1	IPTG				37	4			#DIV/0!
241	238	Charlotte	10/2/2014	pSBX1K3-2Zv2Npu:LaLys+sho2	289			1048C1-2			~0.6		1	IPTG				37	4			#DIV/0!
242	239	Charlotte	10/2/2014	pSBX1K3-2Zv2Npu:LaLys+flexlinker	290			1049C1-3			~0.6		1	IPTG				37	4			#DIV/0!
243	240	Charlotte	10/2/2014	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	102			436C1-13			~0.6		1	IPTG				37	4			#DIV/0!
244	241	Charlotte	10/2/2014	pSBX1K3-2Zv2Npu:LaLys+ord1	296			1114C1			~0.6		1	IPTG				37	4			#DIV/0!
245	242	Charlotte	10/2/2014	pSBX1K3-2Zv2Npu:LaLys+ord3	298			1115C1			~0.6		1	IPTG				37	4			#DIV/0!
246	243	Silvan		pRSFDuet-1-mDNMT1(731-1602), pRARE	23	31		131C1-2	20			16:50	0.4	IPTG				20	18.5		2.4	833.33		incubation temperature was shifted to 20 °C at 16:15 (OD 0.49), 35 min before induction. ZnCl2 was added to a final concentration of 0.1 mM together with IPTG.
247	244	Silvan		pRSFDuet-1-mDNMT1(731-1602), pRARE	23	31		131C1-3	20			16:50	0.4	IPTG				20	18.5		2.4	833.33		incubation temperature was shifted to 20 °C at 16:15 (OD 0.72), 35 min before induction. ZnCl2 was added to a final concentration of 0.1 mM together with IPTG.
248	245	Silvan		pRSFDuet-1-mDNMT1(731-1602), pRARE	23	31		131C2-1	15		0.79	18:30	0.4	IPTG				15			2.46	813.01		incubation temperature was shifted to 15 °C at 18:00 (OD 0.60), 30 min before induction. ZnCl2 was added to a final concentration of 0.1 mM together with IPTG.
249	246	Silvan		pRSFDuet-1-mDNMT1(731-1602), pRARE	23	31		131C2-2	20		0.99	18:30	0.4	IPTG				20			2.26	884.96		incubation temperature was shifted to 20 °C at 18:00 (OD 0.65), 30 min before induction. ZnCl2 was added to a final concentration of 0.1 mM together with IPTG.
250	247	Silvan		pRSFDuet-1-mDNMT1(731-1602), pRARE	23	31		131C2-3	15		0.78	18:55	0.2	IPTG				15			2.72	735.29		incubation temperature was shifted to 15 °C at 18:20 (OD 0.59), 35 min before induction. ZnCl2 was added to a final concentration of 0.1 mM together with IPTG.
251	248	Silvan		pRSFDuet-1-mDNMT1(731-1602), pRARE	23	31		131C2-4	15		0.84	18:55	0.4	IPTG				15			2.04	980.39		incubation temperature was shifted to 15 °C at 18:20 (OD 0.80), 35 min before induction. ZnCl2 was added to a final concentration of 0.1 mM together with IPTG.
252	249	Silvan		pRSFDuet-1-mDNMT1(731-1602), pRARE	23	31		131C2-5	15		0.81	18:55	0.7	IPTG				15			2	1,000.00		incubation temperature was shifted to 15 °C at 18:20 (OD 0.59), 35 min before induction. ZnCl2 was added to a final concentration of 0.1 mM together with IPTG.
253	250	Silvan		pRSFDuet-1-mDNMT1(731-1602), pRARE	23	31		131C2-6	15		0.79	18:55	1	IPTG				15			2.54	787.40		incubation temperature was shifted to 15 °C at 18:20 (OD 0.71), 35 min before induction. ZnCl2 was added to a final concentration of 0.1 mM together with IPTG.
254	251	Silvan		pRSFDuet-1-mDNMT1(731-1602), pRARE	23	31		131C2-7	20		0.87	18:55	0.2	IPTG				20			2.23	896.86		incubation temperature was shifted to 20 °C at 18:20 (OD 0.69), 35 min before induction. ZnCl2 was added to a final concentration of 0.1 mM together with IPTG.
255	252	Silvan		pRSFDuet-1-mDNMT1(731-1602), pRARE	23	31		131C2-8	20		0.87	18:55	0.4	IPTG				20			3.18	628.93		incubation temperature was shifted to 20 °C at 18:20 (OD 0.72), 35 min before induction. ZnCl2 was added to a final concentration of 0.1 mM together with IPTG.
256	253	Silvan		pRSFDuet-1-mDNMT1(731-1602), pRARE	23	31		131C2-9	20		0.82	18:55	0.7	IPTG				20			2.3	869.57		incubation temperature was shifted to 20 °C at 18:20 (OD 0.69), 35 min before induction. ZnCl2 was added to a final concentration of 0.1 mM together with IPTG.
257	254	Silvan		pRSFDuet-1-mDNMT1(731-1602), pRARE	23	31		131C2-10	20		0.84	18:55	1	IPTG				20			3.22	621.12		incubation temperature was shifted to 20 °C at 18:20 (OD 0.68), 35 min before induction. ZnCl2 was added to a final concentration of 0.1 mM together with IPTG.
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Team:Heidelberg/AwesomeSheet/Induktion

From 2014.igem.org

– Induction

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