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From 2014.igem.org

Ran SDS Page gel on incubation products of CsgA Spy Tag and Chromoprotein-Spy Catcher Gibsoned parts of pHBD 68, pHBD 69, pHBD 70, pHBD 72, pHBD 73 and pHBD 74 Transformed Gibsons into Mach I cells Plated transformations