File:LMU14 RFCloning pEBS-cop1.jpg

From 2014.igem.org

http://dx.doi.org/10.1371%2Fjournal.pone.0081370

Establishment of a Markerless Mutation Delivery System in Bacillus subtilis Stimulated by a Double-Strand Break in the Chromosome Ting Shi1, Guanglu Wang, Zhiwen Wang, Jing Fu1, Tao Chen, Xueming Zhao

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current20:49, 17 October 2014Thumbnail for version as of 20:49, 17 October 2014380×304 (22 KB)Patrickreith@gmx.net (Talk | contribs) (http://dx.doi.org/10.1371%2Fjournal.pone.0081370 Establishment of a Markerless Mutation Delivery System in Bacillus subtilis Stimulated by a Double-Strand Break in the Chromosome Ting Shi1, Guanglu Wang, Zhiwen Wang, Jing Fu1, Tao Chen, Xueming Zhao)

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Image title Plasmids for genetic modification in B. subtilis.(A) A modular cassette was cloned into pUC18 to generate pSS, the modular cassette contained chloramphenicol resistance gene (cat), UPRTase encoding gene (upp) and the recognition site of I-SceI endonuclease at 3? end of upp gene. (B) The xylose-inducible I-SceI cassette was cloned into pEB, with a temperature-sensitive replicon (repF) and erythromycin resistance gene (erm).
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